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OPENSEQ.org
MDFA - Multidrug transporter MdfA
UniProt: P0AEY8 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG13696
Length: 410 (374)
Sequences: 66389
Seq/Len: 177.51
MDFA
Paralog alert: 0.90 [within 20: 0.65] - ratio of genomes with paralogs
Cluster includes: AMPG ARAE ARAJ BCR CYNX DGOT DTPA DTPB DTPD EMRB EMRD EMRY ENTS EXUT FSR FUCP GALP GARP GLPT GUDP HCAT HSRA KGTP LACY LPLT MDFA MDTD MDTG MDTH MDTL MDTM MELB MHPT NANT NARK NARU NEPI NUPG PROP RHMT SETA SETB SETC SHIA SOTB TSGA UHPC UHPT UIDB XAPB XYLE YAAU YAGG YAJR YBJJ YCAD YDEE YDFJ YDHC YDHP YDIM YDIN YDJE YDJK YEAN YEBQ YEGT YFCJ YGAY YGCS YHHS YHJE YHJX YICJ YIHN YIHO YIHP YJDL YJHB YJIJ YJJL YNFM YQCE
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
103_Q 107_Q 3.988 1.00
91_W 118_S 3.605 1.00
98_A 111_L 3.485 1.00
100_L 175_V 3.283 1.00
64_G 113_F 2.931 1.00
95_T 111_L 2.85 1.00
72_L 121_F 2.83 1.00
106_E 110_L 2.811 1.00
93_I 181_A 2.786 1.00
81_R 132_E 2.734 1.00
104_N 107_Q 2.715 1.00
134_F 142_I 2.558 1.00
253_S 256_E 2.543 1.00
105_I 109_T 2.513 1.00
88_G 121_F 2.485 1.00
342_S 349_V 2.467 1.00
50_G 53_W 2.449 1.00
140_I 280_S 2.404 1.00
138_V 141_K 2.358 1.00
68_L 117_I 2.319 1.00
95_T 118_S 2.283 1.00
271_G 325_F 2.254 1.00
95_T 114_L 2.237 1.00
229_G 360_I 2.182 1.00
135_E 138_V 2.167 1.00
77_D 347_G 2.163 1.00
82_R 86_L 2.113 1.00
294_P 326_G 2.105 1.00
213_K 217_K 2.093 1.00
267_A 321_S 2.087 1.00
168_L 172_G 2.032 1.00
102_A 107_Q 1.969 1.00
233_L 364_G 1.966 1.00
284_V 340_F 1.939 1.00
102_A 111_L 1.932 1.00
97_L 178_A 1.908 1.00
53_W 105_I 1.908 1.00
86_L 185_F 1.851 1.00
76_S 125_V 1.83 1.00
68_L 120_C 1.827 1.00
301_V 319_G 1.823 1.00
93_I 185_F 1.822 1.00
186_F 190_R 1.797 1.00
86_L 189_Q 1.791 1.00
49_A 105_I 1.789 1.00
95_T 115_Q 1.744 1.00
283_T 286_S 1.736 1.00
314_L 318_A 1.711 1.00
74_P 78_R 1.702 1.00
60_A 109_T 1.697 1.00
57_S 108_F 1.695 1.00
99_I 111_L 1.694 1.00
60_A 113_F 1.663 1.00
80_G 83_P 1.652 1.00
237_A 368_S 1.648 1.00
90_V 185_F 1.643 1.00
64_G 68_L 1.638 1.00
277_R 281_R 1.616 1.00
298_G 319_G 1.577 1.00
49_A 53_W 1.548 1.00
264_I 324_A 1.54 1.00
99_I 108_F 1.528 1.00
252_L 256_E 1.52 1.00
93_I 178_A 1.503 1.00
276_A 280_S 1.502 1.00
175_V 179_A 1.498 1.00
100_L 178_A 1.49 1.00
296_M 300_L 1.485 1.00
225_A 356_L 1.466 1.00
214_L 217_K 1.465 1.00
83_P 87_A 1.457 1.00
75_L 79_I 1.447 1.00
75_L 84_V 1.441 1.00
42_A 45_E 1.435 1.00
23_V 149_V 1.423 1.00
214_L 345_S 1.416 1.00
136_E 139_C 1.415 1.00
267_A 271_G 1.407 0.99
312_A 315_W 1.4 0.99
87_A 91_W 1.393 0.99
97_L 101_L 1.392 0.99
79_I 84_V 1.391 0.99
187_G 191_A 1.389 0.99
107_Q 111_L 1.382 0.99
141_K 280_S 1.38 0.99
98_A 101_L 1.364 0.99
91_W 95_T 1.363 0.99
247_I 257_Y 1.36 0.99
63_A 362_T 1.354 0.99
73_G 77_D 1.349 0.99
106_E 109_T 1.349 0.99
93_I 182_A 1.347 0.99
256_E 259_L 1.343 0.99
68_L 71_L 1.338 0.99
274_L 277_R 1.335 0.99
88_G 118_S 1.333 0.99
247_I 254_S 1.33 0.99
169_P 172_G 1.325 0.99
278_L 282_R 1.324 0.99
51_I 373_L 1.307 0.99
285_R 289_I 1.302 0.99
271_G 274_L 1.287 0.99
130_I 146_M 1.285 0.99
110_L 114_L 1.251 0.99
298_G 326_G 1.248 0.99
275_L 329_L 1.243 0.98
79_I 83_P 1.237 0.98
83_P 86_L 1.235 0.98
131_Q 139_C 1.233 0.98
71_L 74_P 1.231 0.98
286_S 290_M 1.218 0.98
172_G 175_V 1.216 0.98
164_W 172_G 1.207 0.98
166_H 255_Y 1.206 0.98
83_P 194_E 1.206 0.98
159_L 262_V 1.205 0.98
64_G 116_G 1.202 0.98
370_H 374_N 1.198 0.98
286_S 289_I 1.193 0.98
77_D 348_T 1.191 0.98
216_L 222_V 1.181 0.98
268_L 328_G 1.181 0.98
44_V 54_V 1.171 0.98
129_A 192_M 1.168 0.97
94_V 98_A 1.168 0.97
100_L 171_E 1.166 0.97
52_D 373_L 1.166 0.97
314_L 317_T 1.166 0.97
39_G 165_I 1.162 0.97
245_I 250_E 1.16 0.97
179_A 183_I 1.15 0.97
132_E 346_K 1.149 0.97
84_V 87_A 1.146 0.97
159_L 163_A 1.143 0.97
188_L 192_M 1.129 0.97
27_F 153_A 1.127 0.97
98_A 107_Q 1.124 0.97
59_T 366_E 1.115 0.96
59_T 362_T 1.115 0.96
236_L 361_F 1.113 0.96
219_G 222_V 1.111 0.96
291_G 329_L 1.11 0.96
89_V 185_F 1.109 0.96
277_R 280_S 1.106 0.96
80_G 84_V 1.1 0.96
76_S 81_R 1.099 0.96
139_C 340_F 1.098 0.96
291_G 326_G 1.096 0.96
246_I 252_L 1.093 0.96
101_L 175_V 1.092 0.96
73_G 347_G 1.09 0.96
107_Q 110_L 1.089 0.96
56_T 60_A 1.089 0.96
221_F 225_A 1.087 0.96
141_K 145_L 1.086 0.96
345_S 348_T 1.084 0.96
283_T 287_L 1.081 0.95
42_A 46_Q 1.075 0.95
259_L 263_P 1.072 0.95
228_L 353_M 1.071 0.95
266_G 270_A 1.071 0.95
291_G 330_A 1.071 0.95
360_I 364_G 1.071 0.95
222_V 226_L 1.069 0.95
282_R 287_L 1.066 0.95
61_Y 119_L 1.062 0.95
338_T 353_M 1.06 0.95
70_W 74_P 1.055 0.95
66_M 355_M 1.054 0.95
369_K 373_L 1.05 0.94
163_A 167_V 1.044 0.94
56_T 366_E 1.043 0.94
279_T 333_G 1.038 0.94
30_Y 150_A 1.031 0.94
318_A 322_I 1.03 0.94
86_L 90_V 1.022 0.93
46_Q 171_E 1.019 0.93
162_A 258_G 1.019 0.93
219_G 223_A 1.015 0.93
61_Y 116_G 1.014 0.93
44_V 49_A 1.007 0.93
300_L 304_A 1.006 0.93
182_A 186_F 1.005 0.93
255_Y 259_L 1.005 0.93
270_A 274_L 1 0.92
270_A 273_L 1 0.92
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
1pw4A10.99021000.171Contact Map0.502
3wdoA10.95851000.198Contact Map0.623
3o7qA10.96831000.207Contact Map0.636
4j05A20.98781000.209Contact Map0.629
2gfpA20.91461000.213Contact Map0.182
2cfqA10.95371000.24Contact Map0.5
2xutA30.98541000.26Contact Map0.54
4gc0A10.9781000.27Contact Map0.721
4ldsA20.96831000.273Contact Map0.622
4apsA20.98541000.288Contact Map0.558
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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