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OPENSEQ.org
TSGA - Protein TsgA
UniProt: P60778 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG11262
Length: 393 (380)
Sequences: 65928
Seq/Len: 173.49
TSGA
Paralog alert: 0.90 [within 20: 0.66] - ratio of genomes with paralogs
Cluster includes: AMPG ARAE ARAJ BCR CYNX DGOT DTPA DTPB DTPD EMRB EMRD EMRY ENTS EXUT FSR FUCP GALP GARP GLPT GUDP HCAT HSRA KGTP LACY LPLT MDFA MDTD MDTG MDTH MDTL MDTM MELB MHPT NANT NARK NARU NEPI NUPG PROP RHMT SETA SETB SETC SHIA SOTB TSGA UHPC UHPT UIDB XAPB XYLE YAAU YAGG YAJR YBJJ YCAD YDEE YDFJ YDHC YDHP YDIM YDIN YDJE YDJK YEAN YEBQ YEGT YFCJ YGAY YGCS YHHS YHJE YHJX YICJ YIHN YIHO YIHP YJDL YJHB YJIJ YJJL YNFM YQCE
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
94_H 98_L 4.172 1.00
89_G 102_A 3.59 1.00
82_L 109_V 3.558 1.00
91_M 167_A 3.249 1.00
55_G 104_F 3.144 1.00
86_A 102_A 2.962 1.00
97_A 101_A 2.906 1.00
63_N 112_I 2.841 1.00
95_S 98_L 2.794 1.00
72_L 123_Q 2.748 1.00
96_L 100_S 2.627 1.00
239_S 242_D 2.514 1.00
79_G 112_I 2.513 1.00
125_Y 133_R 2.456 1.00
131_G 266_R 2.412 1.00
129_Q 132_S 2.41 1.00
41_P 44_S 2.388 1.00
84_V 173_Y 2.339 1.00
86_A 105_I 2.317 1.00
59_S 108_V 2.308 1.00
86_A 109_V 2.25 1.00
216_L 340_G 2.246 1.00
220_L 344_T 2.24 1.00
73_K 77_R 2.172 1.00
126_E 129_Q 2.152 1.00
68_E 327_P 2.045 1.00
322_T 329_L 2.014 1.00
253_S 301_L 2.004 1.00
160_I 164_W 1.977 1.00
93_S 98_L 1.963 1.00
93_S 102_A 1.933 1.00
44_S 96_L 1.89 1.00
40_L 96_L 1.866 1.00
270_L 320_Q 1.839 1.00
51_F 100_S 1.838 1.00
48_T 99_F 1.836 1.00
51_F 104_F 1.821 1.00
88_A 170_G 1.814 1.00
362_L 366_G 1.814 1.00
67_M 116_I 1.806 1.00
280_L 306_F 1.78 1.00
59_S 111_G 1.77 1.00
86_A 106_L 1.755 1.00
224_G 348_T 1.717 1.00
65_W 69_I 1.71 1.00
90_L 102_A 1.7 1.00
55_G 59_S 1.686 1.00
71_P 74_T 1.68 1.00
14_F 140_F 1.672 1.00
257_G 305_F 1.668 1.00
77_R 177_F 1.656 1.00
282_A 372_F 1.642 1.00
269_D 272_R 1.633 1.00
287_V 299_S 1.628 1.00
90_L 99_F 1.6 1.00
263_F 267_F 1.58 1.00
91_M 170_G 1.539 1.00
40_L 44_S 1.536 1.00
282_A 369_A 1.521 1.00
277_L 309_A 1.499 1.00
81_L 177_F 1.49 1.00
84_V 177_F 1.486 1.00
74_T 78_F 1.47 1.00
98_L 102_A 1.453 1.00
70_V 75_Q 1.438 1.00
54_A 342_M 1.437 1.00
161_E 164_W 1.436 1.00
212_S 336_C 1.432 1.00
66_L 70_V 1.432 1.00
132_S 266_R 1.428 1.00
66_L 75_Q 1.425 1.00
33_N 36_D 1.42 1.00
238_M 242_D 1.42 1.00
89_G 92_F 1.419 1.00
253_S 257_G 1.405 0.99
127_G 130_R 1.387 0.99
78_F 82_L 1.386 0.99
64_A 68_E 1.372 0.99
185_F 188_L 1.372 0.99
97_A 100_S 1.359 0.99
262_S 266_R 1.356 0.99
184_E 187_A 1.355 0.99
121_V 137_T 1.355 0.99
6_R 129_Q 1.355 0.99
284_L 306_F 1.353 0.99
88_A 92_F 1.346 0.99
101_A 105_I 1.346 0.99
250_F 304_G 1.339 0.99
167_A 171_L 1.335 0.99
82_L 86_A 1.334 0.99
84_V 174_V 1.327 0.99
284_L 299_S 1.325 0.99
59_S 62_L 1.324 0.99
242_D 245_T 1.315 0.99
264_I 268_F 1.314 0.99
260_A 263_F 1.301 0.99
74_T 77_R 1.292 0.99
84_V 170_G 1.281 0.99
350_P 354_H 1.272 0.99
208_V 212_S 1.252 0.99
271_Q 275_T 1.251 0.99
62_L 65_W 1.25 0.99
279_G 372_F 1.25 0.99
35_A 45_M 1.244 0.98
279_G 283_I 1.24 0.98
171_L 175_A 1.24 0.98
21_G 141_F 1.237 0.98
233_A 238_M 1.224 0.98
70_V 74_T 1.224 0.98
150_M 248_S 1.222 0.98
16_S 113_T 1.219 0.98
257_G 260_A 1.217 0.98
351_I 355_S 1.209 0.98
155_L 164_W 1.207 0.98
272_R 276_V 1.204 0.98
91_M 163_Y 1.204 0.98
275_T 372_F 1.202 0.98
68_E 328_K 1.199 0.98
122_T 130_R 1.199 0.98
30_V 156_L 1.198 0.98
79_G 109_V 1.19 0.98
42_V 353_E 1.19 0.98
75_Q 78_F 1.189 0.98
232_Y 236_L 1.185 0.98
67_M 72_L 1.185 0.98
272_R 275_T 1.181 0.98
164_W 167_A 1.18 0.98
298_W 302_A 1.178 0.98
359_A 362_L 1.177 0.98
33_N 37_Y 1.171 0.98
50_T 342_M 1.169 0.97
85_L 89_G 1.164 0.97
282_A 365_N 1.16 0.97
366_G 370_V 1.145 0.97
55_G 107_G 1.144 0.97
68_E 72_L 1.14 0.97
174_V 178_I 1.138 0.97
150_M 154_F 1.136 0.97
280_L 284_L 1.133 0.97
340_G 344_T 1.13 0.97
223_L 341_T 1.129 0.97
71_P 75_Q 1.121 0.97
184_E 188_L 1.121 0.97
157_A 241_N 1.118 0.96
89_G 98_L 1.118 0.96
47_N 51_F 1.116 0.96
57_L 335_T 1.113 0.96
261_F 309_A 1.109 0.96
284_L 303_L 1.107 0.96
282_A 286_Y 1.102 0.96
52_L 110_S 1.098 0.96
92_F 167_A 1.096 0.96
47_N 346_V 1.096 0.96
37_Y 163_Y 1.089 0.96
295_H 298_W 1.087 0.96
98_L 101_A 1.086 0.96
349_G 353_E 1.085 0.96
170_G 174_V 1.083 0.96
18_A 144_A 1.082 0.96
252_M 256_V 1.079 0.95
245_T 249_N 1.078 0.95
355_S 359_A 1.076 0.95
51_F 55_G 1.076 0.95
233_A 296_M 1.074 0.95
132_S 136_F 1.069 0.95
209_L 213_V 1.064 0.95
171_L 174_V 1.056 0.95
263_F 266_R 1.056 0.95
221_G 368_Y 1.054 0.95
218_Y 310_I 1.05 0.94
183_C 187_A 1.05 0.94
278_A 371_V 1.046 0.94
153_A 244_G 1.045 0.94
50_T 346_V 1.044 0.94
286_Y 365_N 1.044 0.94
35_A 40_L 1.037 0.94
43_S 353_E 1.035 0.94
77_R 81_L 1.034 0.94
281_A 368_Y 1.026 0.94
318_G 333_V 1.025 0.93
61_F 65_W 1.025 0.93
132_S 263_F 1.019 0.93
231_E 234_K 1.017 0.93
297_A 301_L 1.016 0.93
35_A 42_V 1.015 0.93
256_V 260_A 1.014 0.93
16_S 76_L 1.011 0.93
215_A 333_V 1.011 0.93
200_E 203_K 1.01 0.93
185_F 189_G 1.004 0.93
145_G 251_W 1.003 0.93
269_D 273_I 1 0.92
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
1pw4A10.99491000.167Contact Map0.491
4j05A20.99491000.174Contact Map0.617
3o7qA10.97461000.189Contact Map0.629
3wdoA10.98731000.194Contact Map0.635
2gfpA20.93381000.208Contact Map0.182
4ldsA20.99491000.219Contact Map0.637
4gc0A111000.221Contact Map0.704
2cfqA10.98731000.227Contact Map0.498
2xutA30.99751000.239Contact Map0.521
4apsA20.99491000.269Contact Map0.544
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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