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NEPI - Purine ribonucleoside efflux pump NepI
UniProt: P0ADL1 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG11689
Length: 396 (369)
Sequences: 64191
Seq/Len: 173.96
NEPI
Paralog alert: 0.90 [within 20: 0.64] - ratio of genomes with paralogs
Cluster includes: AMPG ARAE ARAJ BCR CYNX DGOT DTPA DTPB DTPD EMRB EMRD EMRY ENTS EXUT FSR FUCP GALP GARP GLPT GUDP HCAT HSRA KGTP LACY LPLT MDFA MDTD MDTG MDTH MDTL MDTM MELB MHPT NANT NARK NARU NEPI NUPG PROP RHMT SETA SETB SETC SHIA SOTB TSGA UHPC UHPT UIDB XAPB XYLE YAAU YAGG YAJR YBJJ YCAD YDEE YDFJ YDHC YDHP YDIM YDIN YDJE YDJK YEAN YEBQ YEGT YFCJ YGAY YGCS YHHS YHJE YHJX YICJ YIHN YIHO YIHP YJDL YJHB YJIJ YJJL YNFM YQCE
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
106_N 110_L 4.175 1.00
94_L 121_A 3.53 1.00
101_L 114_G 3.462 1.00
103_S 178_N 3.218 1.00
67_V 116_A 2.92 1.00
109_S 113_I 2.87 1.00
96_T 184_G 2.865 1.00
98_S 114_G 2.834 1.00
75_I 124_G 2.744 1.00
84_R 135_R 2.717 1.00
107_S 110_L 2.7 1.00
137_V 145_A 2.516 1.00
108_F 112_L 2.494 1.00
250_G 253_G 2.483 1.00
143_P 277_K 2.451 1.00
53_S 56_V 2.404 1.00
91_F 124_G 2.401 1.00
141_T 144_K 2.354 1.00
98_S 121_A 2.292 1.00
98_S 117_C 2.285 1.00
71_A 120_L 2.28 1.00
80_Q 339_E 2.143 1.00
264_A 313_I 2.127 1.00
138_P 141_T 2.082 1.00
268_G 317_L 2.077 1.00
230_A 356_G 2.045 1.00
85_R 89_I 2.031 1.00
105_A 110_L 1.997 1.00
226_F 352_A 1.99 1.00
171_I 175_N 1.972 1.00
89_I 192_I 1.952 1.00
210_F 214_Q 1.947 1.00
105_A 114_G 1.905 1.00
189_F 193_K 1.889 1.00
100_L 181_A 1.865 1.00
56_V 108_F 1.858 1.00
306_I 310_G 1.846 1.00
63_V 116_A 1.823 1.00
89_I 188_I 1.813 1.00
60_S 111_L 1.793 1.00
52_I 108_F 1.774 1.00
63_V 112_L 1.77 1.00
297_V 311_V 1.767 1.00
98_S 118_L 1.76 1.00
234_A 360_G 1.758 1.00
71_A 123_G 1.726 1.00
93_V 188_I 1.705 1.00
281_K 332_R 1.697 1.00
96_T 188_I 1.695 1.00
374_M 378_T 1.665 1.00
79_I 128_M 1.662 1.00
77_Q 81_A 1.655 1.00
83_D 86_Y 1.654 1.00
102_V 114_G 1.648 1.00
67_V 71_A 1.636 1.00
211_R 214_Q 1.623 1.00
52_I 56_V 1.616 1.00
274_F 278_R 1.595 1.00
102_V 111_L 1.58 1.00
45_P 48_Q 1.524 1.00
222_M 348_V 1.519 1.00
334_L 341_A 1.509 1.00
96_T 181_A 1.505 1.00
110_L 114_G 1.501 1.00
280_V 283_A 1.497 1.00
249_F 253_G 1.483 1.00
86_Y 90_L 1.468 1.00
178_N 182_V 1.458 1.00
190_W 194_S 1.45 1.00
211_R 337_Q 1.447 1.00
78_T 87_V 1.447 1.00
144_K 277_K 1.444 1.00
26_A 152_A 1.427 1.00
82_T 87_V 1.425 1.00
103_S 181_A 1.423 1.00
78_T 82_T 1.419 1.00
253_G 256_L 1.408 1.00
100_L 104_F 1.4 0.99
90_L 94_L 1.398 0.99
139_P 142_V 1.389 0.99
109_S 112_L 1.387 0.99
101_L 104_F 1.384 0.99
294_S 311_V 1.383 0.99
264_A 268_G 1.38 0.99
362_Y 366_N 1.37 0.99
71_A 74_F 1.36 0.99
273_S 277_K 1.356 0.99
172_G 175_N 1.343 0.99
244_M 254_L 1.331 0.99
76_T 80_Q 1.323 0.99
94_L 98_S 1.323 0.99
261_F 316_G 1.321 0.99
66_F 354_T 1.308 0.99
275_I 279_S 1.302 0.99
113_I 117_C 1.289 0.99
304_D 307_V 1.288 0.99
244_M 251_V 1.282 0.99
162_P 259_L 1.275 0.99
271_L 274_F 1.273 0.99
82_T 86_Y 1.265 0.99
86_Y 89_I 1.259 0.99
74_F 77_Q 1.249 0.99
175_N 178_N 1.234 0.98
133_T 149_I 1.232 0.98
134_M 142_V 1.232 0.98
54_E 365_D 1.229 0.98
91_F 121_A 1.227 0.98
96_T 185_V 1.226 0.98
306_I 309_T 1.224 0.98
294_S 318_T 1.222 0.98
218_V 222_M 1.21 0.98
135_R 338_A 1.209 0.98
231_G 380_M 1.203 0.98
80_Q 340_K 1.203 0.98
86_Y 197_S 1.2 0.98
283_A 287_A 1.188 0.98
291_L 318_T 1.184 0.98
363_A 367_I 1.18 0.98
97_L 101_L 1.174 0.98
42_L 168_G 1.17 0.97
352_A 356_G 1.168 0.97
132_L 195_L 1.163 0.97
79_I 84_R 1.16 0.97
87_V 90_L 1.155 0.97
103_S 174_R 1.154 0.97
268_G 271_L 1.148 0.97
367_I 371_S 1.144 0.97
47_A 57_A 1.144 0.97
167_L 175_N 1.143 0.97
169_E 252_D 1.137 0.97
92_A 188_I 1.137 0.97
83_D 87_V 1.135 0.97
67_V 119_G 1.131 0.97
242_V 247_A 1.126 0.97
219_M 223_I 1.12 0.96
274_F 277_K 1.119 0.96
62_T 354_T 1.117 0.96
213_L 219_M 1.115 0.96
182_V 186_L 1.115 0.96
378_T 382_L 1.111 0.96
110_L 113_I 1.109 0.96
101_L 110_L 1.108 0.96
45_P 49_D 1.108 0.96
272_S 321_L 1.107 0.96
69_M 347_A 1.1 0.96
55_G 365_D 1.092 0.96
28_C 125_F 1.09 0.96
64_T 68_A 1.086 0.96
295_A 299_T 1.084 0.96
162_P 166_F 1.084 0.96
243_Y 249_F 1.082 0.96
59_Q 358_A 1.082 0.96
293_V 297_V 1.077 0.95
104_F 178_N 1.075 0.95
64_T 119_G 1.073 0.95
33_I 153_V 1.071 0.95
252_D 256_L 1.069 0.95
361_G 365_D 1.055 0.95
292_A 384_A 1.054 0.95
59_Q 63_V 1.053 0.95
42_L 176_V 1.052 0.95
225_I 345_Q 1.052 0.95
80_Q 84_R 1.052 0.95
89_I 93_V 1.051 0.94
157_L 262_G 1.051 0.94
216_P 219_M 1.047 0.94
185_V 189_F 1.043 0.94
282_L 286_G 1.038 0.94
263_I 267_I 1.035 0.94
144_K 148_V 1.033 0.94
310_G 314_I 1.031 0.94
62_T 358_A 1.03 0.94
166_F 170_L 1.03 0.94
76_T 339_E 1.027 0.94
191_I 195_L 1.018 0.93
30_A 156_A 1.014 0.93
337_Q 340_K 1.01 0.93
256_L 260_S 1.01 0.93
370_T 374_M 1.008 0.93
58_G 62_T 1.004 0.93
330_I 345_Q 1 0.92
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
1pw4A10.99751000.183Contact Map0.488
3wdoA10.96721000.208Contact Map0.631
4j05A20.99491000.213Contact Map0.625
3o7qA10.99241000.213Contact Map0.617
2gfpA20.93181000.228Contact Map0.192
2cfqA10.95711000.265Contact Map0.482
4jr9A10.98741000.284Contact Map0.603
2xutA30.97981000.294Contact Map0.548
4ldsA20.96211000.305Contact Map0.624
4gc0A10.97471000.311Contact Map0.717
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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