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OPENSEQ.org
MELB - Melibiose carrier protein
UniProt: P02921 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG10578
Length: 473 (428)
Sequences: 23605
Seq/Len: 55.15
MELB
Paralog alert: 0.83 [within 20: 0.30] - ratio of genomes with paralogs
Cluster includes: AMPG ARAE ARAJ BCR CYNX DGOT DTPA DTPB DTPD EMRB EMRD EMRY ENTS EXUT FSR FUCP GALP GARP GLPT GUDP HCAT HSRA KGTP LACY LPLT MDFA MDTD MDTG MDTH MDTL MDTM MELB MHPT NANT NARK NARU NEPI NUPG PROP RHMT SETA SETB SETC SHIA SOTB TSGA UHPC UHPT UIDB XAPB XYLE YAAU YAGG YAJR YBJJ YCAD YDEE YDFJ YDHC YDHP YDIM YDIN YDJE YDJK YEAN YEBQ YEGT YFCJ YGAY YGCS YHHS YHJE YHJX YICJ YIHN YIHO YIHP YJDL YJHB YJIJ YJJL YNFM YQCE
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
98_H 108_F 4.302 1.00
107_V 111_V 4.146 1.00
86_A 119_T 3.458 1.00
353_V 368_A 3.236 1.00
88_S 190_F 3.115 1.00
95_F 184_L 3.002 1.00
93_L 112_T 2.928 1.00
106_I 110_C 2.787 1.00
90_I 112_T 2.771 1.00
142_E 291_K 2.718 1.00
54_W 114_I 2.676 1.00
305_L 337_G 2.664 1.00
62_M 122_I 2.636 1.00
40_S 43_L 2.616 1.00
307_V 423_M 2.546 1.00
282_T 336_V 2.436 1.00
302_A 340_L 2.385 1.00
99_L 108_F 2.354 1.00
325_V 329_I 2.329 1.00
92_F 96_S 2.323 1.00
97_A 108_F 2.31 1.00
309_S 330_A 2.305 1.00
90_I 115_L 2.303 1.00
83_G 122_I 2.249 1.00
225_A 229_K 2.229 1.00
136_L 140_E 2.177 1.00
58_N 121_T 2.158 1.00
67_N 366_S 2.131 1.00
312_V 330_A 2.126 1.00
81_L 194_T 2.051 1.00
295_R 351_D 2.049 1.00
300_A 426_L 2.041 1.00
90_I 119_T 2.041 1.00
53_I 381_A 2.035 1.00
58_N 118_M 2.034 1.00
99_L 107_V 2.032 1.00
311_G 419_T 2.025 1.00
50_V 110_C 1.975 1.00
304_I 426_L 1.935 1.00
309_S 337_G 1.898 1.00
66_V 126_P 1.878 1.00
278_A 332_I 1.875 1.00
43_L 106_I 1.858 1.00
75_K 133_T 1.848 1.00
81_L 198_L 1.846 1.00
135_T 144_L 1.822 1.00
287_P 291_K 1.814 1.00
97_A 112_T 1.796 1.00
47_L 109_V 1.748 1.00
76_F 81_L 1.706 1.00
307_V 311_G 1.696 1.00
92_F 187_I 1.689 1.00
356_G 364_C 1.679 1.00
50_V 114_I 1.677 1.00
241_L 379_G 1.667 1.00
136_L 139_R 1.649 1.00
177_F 181_M 1.64 1.00
93_L 96_S 1.625 1.00
390_V 394_I 1.625 1.00
311_G 315_L 1.602 1.00
64_W 68_A 1.598 1.00
278_A 282_T 1.596 1.00
94_L 112_T 1.578 1.00
161_G 273_S 1.571 1.00
12_G 151_F 1.551 1.00
54_W 58_N 1.545 1.00
16_F 158_V 1.541 1.00
85_L 194_T 1.523 1.00
140_E 143_Q 1.523 1.00
39_L 106_I 1.521 1.00
221_K 225_A 1.514 1.00
63_G 366_S 1.503 1.00
304_I 308_L 1.497 1.00
354_D 364_C 1.481 1.00
111_V 115_L 1.478 1.00
88_S 194_T 1.477 1.00
68_A 73_W 1.46 1.00
296_R 300_A 1.458 1.00
389_V 393_M 1.457 1.00
307_V 426_L 1.455 1.00
77_K 81_L 1.44 1.00
77_K 203_E 1.439 1.00
226_L 356_G 1.434 1.00
290_V 344_L 1.414 1.00
245_V 383_A 1.394 0.99
231_D 235_C 1.384 0.99
294_S 297_I 1.374 0.99
74_G 203_E 1.372 0.99
275_A 335_N 1.364 0.99
195_I 199_R 1.356 0.99
75_K 203_E 1.351 0.99
49_L 385_F 1.35 0.99
108_F 112_T 1.349 0.99
86_A 90_I 1.349 0.99
423_M 427_I 1.347 0.99
56_A 374_M 1.344 0.99
90_I 116_W 1.343 0.99
65_I 78_P 1.342 0.99
386_F 389_V 1.337 0.99
285_F 288_R 1.327 0.99
65_I 73_W 1.323 0.99
420_L 424_V 1.312 0.99
258_F 262_I 1.307 0.99
439_T 442_R 1.3 0.99
27_M 163_T 1.299 0.99
252_G 255_I 1.298 0.99
231_D 234_S 1.294 0.99
49_L 381_A 1.294 0.99
297_I 301_G 1.294 0.99
141_R 351_D 1.293 0.99
288_R 292_S 1.293 0.99
88_S 191_I 1.286 0.99
302_A 337_G 1.282 0.99
39_L 43_L 1.276 0.99
93_L 108_F 1.272 0.99
131_V 144_L 1.265 0.99
46_T 50_V 1.264 0.99
143_Q 291_K 1.262 0.99
379_G 383_A 1.257 0.99
248_N 380_S 1.255 0.99
66_V 75_K 1.246 0.98
308_L 312_V 1.243 0.98
73_W 77_K 1.241 0.98
289_L 298_L 1.232 0.98
89_V 93_L 1.232 0.98
303_S 341_F 1.232 0.98
228_Y 234_S 1.229 0.98
296_R 430_F 1.225 0.98
301_G 305_L 1.214 0.98
95_F 187_I 1.211 0.98
277_A 281_V 1.21 0.98
24_I 182_F 1.209 0.98
10_S 200_N 1.208 0.98
284_V 288_R 1.203 0.98
150_F 280_L 1.199 0.98
286_F 340_L 1.196 0.98
289_L 293_L 1.193 0.98
108_F 111_V 1.193 0.98
153_S 279_N 1.189 0.98
77_K 82_I 1.189 0.98
56_A 378_G 1.187 0.98
181_M 185_V 1.186 0.98
293_L 298_L 1.177 0.98
303_S 426_L 1.167 0.97
130_L 201_V 1.166 0.97
157_F 277_A 1.159 0.97
257_Y 261_V 1.159 0.97
83_G 119_T 1.159 0.97
94_L 109_V 1.158 0.97
254_A 257_Y 1.157 0.97
67_N 75_K 1.153 0.97
16_F 155_A 1.153 0.97
267_L 270_Y 1.151 0.97
82_I 86_A 1.143 0.97
28_Y 182_F 1.137 0.97
55_D 377_K 1.137 0.97
348_M 433_Y 1.134 0.97
41_V 392_G 1.131 0.97
43_L 110_C 1.131 0.97
84_T 193_S 1.129 0.97
16_F 151_F 1.124 0.97
74_G 77_K 1.123 0.97
88_S 187_I 1.121 0.97
73_W 78_P 1.117 0.96
31_Y 35_D 1.113 0.96
166_F 181_M 1.11 0.96
46_T 385_F 1.105 0.96
326_L 329_I 1.096 0.96
84_T 194_T 1.088 0.96
54_W 117_G 1.088 0.96
67_N 367_I 1.084 0.96
388_A 392_G 1.084 0.96
181_M 184_L 1.084 0.96
256_Y 260_Y 1.075 0.95
297_I 300_A 1.071 0.95
405_Q 409_G 1.071 0.95
329_I 333_L 1.07 0.95
250_I 257_Y 1.07 0.95
197_T 201_V 1.069 0.95
253_F 257_Y 1.068 0.95
112_T 115_L 1.068 0.95
329_I 332_I 1.065 0.95
249_I 387_I 1.063 0.95
96_S 108_F 1.061 0.95
427_I 431_R 1.059 0.95
63_G 67_N 1.058 0.95
95_F 180_Q 1.055 0.95
234_S 238_G 1.054 0.95
309_S 334_L 1.053 0.95
21_A 24_I 1.053 0.95
304_I 307_V 1.053 0.95
85_L 89_V 1.05 0.94
257_Y 262_I 1.046 0.94
162_V 165_P 1.044 0.94
154_L 280_L 1.039 0.94
222_A 225_A 1.039 0.94
91_L 187_I 1.038 0.94
184_L 188_A 1.034 0.94
145_V 347_I 1.031 0.94
300_A 304_I 1.029 0.94
250_I 254_A 1.023 0.93
107_V 110_C 1.022 0.93
226_L 229_K 1.02 0.93
96_S 184_L 1.016 0.93
382_F 386_F 1.011 0.93
51_A 55_D 1.01 0.93
56_A 381_A 1.01 0.93
415_I 420_L 1.009 0.93
181_M 188_A 1.007 0.93
13_F 193_S 1.007 0.93
226_L 364_C 1.006 0.93
270_Y 274_Y 1.006 0.93
138_K 141_R 1.005 0.93
314_L 415_I 1.003 0.93
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
3wdoA10.88791000.427Contact Map0.605
1pw4A10.85411000.443Contact Map0.505
4j05A20.90491000.453Contact Map0.635
4gc0A10.90491000.461Contact Map0.77
2cfqA10.86051000.469Contact Map0.52
2gfpA20.79071000.477Contact Map0.148
4ldsA20.8711000.482Contact Map0.675
3o7qA10.82881000.487Contact Map0.632
2xutA30.89641000.5Contact Map0.51
4lepA20.94711000.504Contact Map0.596
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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