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OPENSEQ.org
NARK - Nitrate/nitrite transporter NarK
UniProt: P10903 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG10642
Length: 463 (383)
Sequences: 40635
Seq/Len: 106.10
NARK
Paralog alert: 0.90 [within 20: 0.53] - ratio of genomes with paralogs
Cluster includes: AMPG ARAE ARAJ BCR CYNX DGOT DTPA DTPB DTPD EMRB EMRD EMRY ENTS EXUT FSR FUCP GALP GARP GLPT GUDP HCAT HSRA KGTP LACY LPLT MDFA MDTD MDTG MDTH MDTL MDTM MELB MHPT NANT NARK NARU NEPI NUPG PROP RHMT SETA SETB SETC SHIA SOTB TSGA UHPC UHPT UIDB XAPB XYLE YAAU YAGG YAJR YBJJ YCAD YDEE YDFJ YDHC YDHP YDIM YDIN YDJE YDJK YEAN YEBQ YEGT YFCJ YGAY YGCS YHHS YHJE YHJX YICJ YIHN YIHO YIHP YJDL YJHB YJIJ YJJL YNFM YQCE
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
128_T 132_V 3.988 1.00
111_I 143_A 3.427 1.00
120_G 217_W 3.314 1.00
131_S 135_I 3.077 1.00
118_W 136_I 2.977 1.00
84_S 138_L 2.932 1.00
113_I 223_L 2.85 1.00
164_G 313_D 2.776 1.00
129_P 132_V 2.753 1.00
115_P 136_I 2.753 1.00
130_Y 134_I 2.537 1.00
70_T 73_Q 2.347 1.00
101_G 156_F 2.281 1.00
158_F 166_A 2.255 1.00
440_K 444_I 2.247 1.00
97_P 404_A 2.18 1.00
162_K 165_G 2.171 1.00
115_P 139_L 2.144 1.00
115_P 143_A 2.137 1.00
159_P 162_K 2.117 1.00
117_V 220_V 2.114 1.00
88_L 142_F 2.113 1.00
248_L 252_K 2.112 1.00
264_L 417_G 2.087 1.00
327_L 361_T 2.077 1.00
122_A 136_I 1.996 1.00
106_A 227_T 1.99 1.00
286_P 289_Q 1.97 1.00
122_A 132_V 1.954 1.00
88_L 145_A 1.928 1.00
329_A 447_I 1.909 1.00
268_G 421_I 1.86 1.00
115_P 140_C 1.854 1.00
80_L 138_L 1.852 1.00
228_I 232_F 1.827 1.00
349_M 353_A 1.827 1.00
77_L 133_F 1.783 1.00
96_V 149_S 1.781 1.00
329_A 450_V 1.781 1.00
102_R 106_A 1.781 1.00
80_L 134_I 1.766 1.00
334_L 354_V 1.765 1.00
110_G 227_T 1.759 1.00
377_F 406_A 1.732 1.00
106_A 231_W 1.732 1.00
113_I 227_T 1.724 1.00
94_F 98_I 1.72 1.00
326_I 450_V 1.693 1.00
316_G 319_R 1.691 1.00
272_G 425_F 1.679 1.00
73_Q 130_Y 1.678 1.00
317_G 375_V 1.644 1.00
83_V 419_F 1.635 1.00
427_S 431_L 1.616 1.00
331_F 354_V 1.603 1.00
95_M 104_W 1.6 1.00
310_A 314_R 1.59 1.00
309_G 313_D 1.588 1.00
336_F 440_K 1.579 1.00
249_P 252_K 1.579 1.00
100_G 103_R 1.574 1.00
119_L 136_I 1.564 1.00
197_F 214_N 1.564 1.00
84_S 88_L 1.54 1.00
322_L 450_V 1.538 1.00
103_R 107_F 1.536 1.00
119_L 133_F 1.523 1.00
118_W 121_F 1.516 1.00
93_S 97_P 1.503 1.00
95_M 99_F 1.481 1.00
191_L 197_F 1.478 1.00
69_F 130_Y 1.473 1.00
42_V 173_L 1.469 1.00
79_A 419_F 1.462 1.00
61_N 64_K 1.45 1.00
69_F 73_Q 1.443 1.00
99_F 103_R 1.434 1.00
333_G 443_L 1.424 1.00
183_L 295_F 1.411 1.00
113_I 220_V 1.41 1.00
269_S 446_Y 1.402 0.99
217_W 221_P 1.401 0.99
99_F 104_W 1.394 0.99
117_V 121_F 1.393 0.99
326_I 330_I 1.392 0.99
165_G 313_D 1.379 0.99
260_S 413_I 1.365 0.99
378_R 402_D 1.344 0.99
34_A 162_K 1.342 0.99
255_H 258_I 1.328 0.99
329_A 333_G 1.325 0.99
329_A 443_L 1.325 0.99
93_S 404_A 1.324 0.99
304_A 360_L 1.319 0.99
88_L 91_P 1.319 0.99
347_S 350_A 1.314 0.99
154_I 170_N 1.308 0.99
120_G 220_V 1.306 0.99
307_A 310_A 1.303 0.99
311_L 315_L 1.3 0.99
111_I 115_P 1.288 0.99
289_Q 292_Q 1.281 0.99
122_A 128_T 1.277 0.99
300_I 304_A 1.277 0.99
444_I 448_A 1.276 0.99
103_R 236_D 1.271 0.99
183_L 187_L 1.268 0.99
71_T 430_A 1.267 0.99
103_R 106_A 1.266 0.99
282_K 290_I 1.254 0.99
132_V 136_I 1.25 0.99
135_I 139_L 1.249 0.99
114_I 118_W 1.239 0.98
229_A 233_G 1.23 0.98
107_F 111_I 1.229 0.98
271_I 418_G 1.221 0.98
108_S 143_A 1.217 0.98
132_V 135_I 1.217 0.98
109_T 227_T 1.201 0.98
81_P 85_G 1.197 0.98
336_F 443_L 1.194 0.98
230_A 234_M 1.193 0.98
417_G 421_I 1.19 0.98
318_T 322_L 1.19 0.98
160_K 163_Q 1.187 0.98
428_S 432_T 1.184 0.98
300_I 356_L 1.181 0.98
214_N 217_W 1.169 0.97
426_G 430_A 1.168 0.97
327_L 331_F 1.161 0.97
436_V 440_K 1.155 0.97
282_K 287_D 1.153 0.97
224_A 228_I 1.148 0.97
319_R 323_V 1.146 0.97
120_G 213_A 1.144 0.97
437_G 440_K 1.141 0.97
246_E 249_P 1.14 0.97
371_Q 375_V 1.136 0.97
249_P 402_D 1.135 0.97
265_A 449_C 1.128 0.97
97_P 405_A 1.127 0.97
113_I 224_A 1.127 0.97
198_G 214_N 1.126 0.97
131_S 134_I 1.126 0.97
104_W 107_F 1.125 0.97
100_G 104_W 1.123 0.97
153_N 234_M 1.118 0.96
361_T 365_G 1.111 0.96
437_G 441_V 1.109 0.96
254_G 257_W 1.109 0.96
292_Q 296_F 1.108 0.96
304_A 307_A 1.106 0.96
63_P 74_L 1.101 0.96
447_I 451_V 1.098 0.96
333_G 337_L 1.093 0.96
50_C 181_M 1.092 0.96
72_D 430_A 1.089 0.96
329_A 446_Y 1.087 0.96
254_G 258_I 1.084 0.96
77_L 130_Y 1.08 0.95
76_M 423_K 1.076 0.95
432_T 437_G 1.072 0.95
100_G 236_D 1.069 0.95
220_V 224_A 1.068 0.95
187_L 191_L 1.066 0.95
66_G 213_A 1.061 0.95
325_F 450_V 1.061 0.95
38_L 158_F 1.055 0.95
97_P 101_G 1.053 0.95
198_G 213_A 1.051 0.94
91_P 94_F 1.049 0.94
50_C 219_W 1.047 0.94
306_S 310_A 1.043 0.94
73_Q 134_I 1.041 0.94
325_F 449_C 1.041 0.94
221_P 225_I 1.039 0.94
322_L 454_W 1.038 0.94
257_W 261_L 1.037 0.94
378_R 401_T 1.037 0.94
190_S 288_V 1.037 0.94
323_V 327_L 1.035 0.94
128_T 213_A 1.032 0.94
328_M 446_Y 1.031 0.94
299_F 303_L 1.023 0.93
441_V 444_I 1.022 0.93
184_V 187_L 1.022 0.93
110_G 114_I 1.021 0.93
156_F 403_T 1.019 0.93
122_A 129_P 1.017 0.93
249_P 401_T 1.015 0.93
188_V 214_N 1.013 0.93
164_G 309_G 1.012 0.93
256_L 260_S 1.005 0.93
121_F 217_W 1.004 0.93
165_G 169_L 1.004 0.93
102_R 236_D 1 0.92
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
4jr9A10.98271000.319Contact Map0.61
1pw4A10.87261000.331Contact Map0.471
3wdoA10.82941000.349Contact Map0.626
4j05A20.90931000.35Contact Map0.623
3o7qA10.88551000.367Contact Map0.604
2gfpA20.79271000.384Contact Map0.174
2cfqA10.82941000.391Contact Map0.505
4ldsA20.83591000.406Contact Map0.63
2xutA30.84671000.414Contact Map0.511
4gc0A10.8511000.418Contact Map0.712
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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