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OPENSEQ.org
YIFK - Probable transport protein YifK
UniProt: P27837 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG11459
Length: 461 (445)
Sequences: 10848
Seq/Len: 24.38
YIFK
Paralog alert: 0.82 [within 20: 0.39] - ratio of genomes with paralogs
Cluster includes: ADIC ANSP ARCD AROP CADB CYCA FRLA GABP GADC LYSP MMUP PHEP PLAP POTE PROY PUUP YBAT YCAM YGJI YIFK YJEH YJEM
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
322_A 334_A 4.145 1.00
376_F 411_M 3.622 1.00
136_A 340_I 3.444 1.00
168_G 254_V 3.303 1.00
314_A 324_A 3.237 1.00
13_E 227_K 3.066 1.00
231_V 235_S 2.975 1.00
325_K 334_A 2.964 1.00
53_G 245_L 2.937 1.00
228_N 231_V 2.863 1.00
384_R 388_A 2.852 1.00
129_L 347_S 2.642 1.00
379_L 410_T 2.61 1.00
337_A 341_A 2.432 1.00
408_Y 412_A 2.412 1.00
317_R 321_A 2.4 1.00
282_A 286_I 2.391 1.00
322_A 325_K 2.351 1.00
226_A 232_T 2.305 1.00
311_Y 315_K 2.303 1.00
93_A 313_L 2.263 1.00
327_S 333_V 2.214 1.00
14_A 239_K 2.155 1.00
54_L 409_V 2.14 1.00
44_S 202_F 2.088 1.00
18_E 239_K 2.087 1.00
62_S 379_L 2.087 1.00
14_A 232_T 2.087 1.00
77_A 316_N 2.057 1.00
323_M 338_V 2.056 1.00
224_G 315_K 2.029 1.00
81_H 89_G 1.999 1.00
13_E 16_H 1.987 1.00
81_H 86_P 1.947 1.00
225_E 308_R 1.936 1.00
8_L 326_V 1.934 1.00
260_P 263_E 1.914 1.00
65_E 386_R 1.889 1.00
384_R 387_R 1.869 1.00
263_E 277_K 1.865 1.00
54_L 58_F 1.862 1.00
84_M 380_I 1.859 1.00
136_A 336_V 1.853 1.00
140_L 333_V 1.837 1.00
84_M 384_R 1.833 1.00
87_F 445_Y 1.829 1.00
412_A 416_C 1.792 1.00
405_W 409_V 1.787 1.00
140_L 337_A 1.783 1.00
235_S 239_K 1.771 1.00
17_I 226_A 1.769 1.00
48_A 205_A 1.765 1.00
155_M 159_T 1.761 1.00
97_W 339_S 1.729 1.00
158_V 162_I 1.726 1.00
144_R 148_E 1.715 1.00
14_A 18_E 1.703 1.00
137_L 141_A 1.701 1.00
380_I 411_M 1.698 1.00
228_N 232_T 1.692 1.00
166_V 170_G 1.687 1.00
129_L 344_L 1.65 1.00
402_L 406_A 1.643 1.00
37_T 45_V 1.635 1.00
228_N 235_S 1.625 1.00
16_H 151_F 1.623 1.00
132_V 343_L 1.621 1.00
80_A 92_T 1.618 1.00
62_S 407_N 1.617 1.00
92_T 377_V 1.588 1.00
443_A 447_V 1.57 1.00
12_L 16_H 1.558 1.00
133_A 344_L 1.552 1.00
229_P 234_R 1.548 1.00
327_S 331_V 1.545 1.00
307_G 336_V 1.529 1.00
238_G 242_W 1.521 1.00
435_I 439_L 1.512 1.00
58_F 409_V 1.503 1.00
306_C 339_S 1.502 1.00
310_L 335_G 1.5 1.00
55_F 413_F 1.496 1.00
18_E 243_R 1.49 1.00
334_A 338_V 1.489 1.00
68_F 397_P 1.489 1.00
50_I 249_V 1.486 1.00
439_L 443_A 1.486 1.00
383_L 408_Y 1.482 1.00
222_T 304_Y 1.462 1.00
382_Q 407_N 1.45 1.00
273_L 276_A 1.448 1.00
115_V 127_P 1.448 1.00
242_W 246_I 1.441 1.00
80_A 377_V 1.433 1.00
12_L 147_G 1.427 1.00
321_A 325_K 1.427 1.00
321_A 324_A 1.42 1.00
139_N 300_N 1.415 1.00
85_S 88_F 1.396 0.99
159_T 163_V 1.394 0.99
404_P 408_Y 1.389 0.99
84_M 88_F 1.389 0.99
22_L 243_R 1.381 0.99
436_I 439_L 1.364 0.99
411_M 415_I 1.36 0.99
44_S 195_F 1.357 0.99
432_F 436_I 1.353 0.99
329_H 333_V 1.35 0.99
14_A 235_S 1.344 0.99
419_I 423_F 1.343 0.99
380_I 384_R 1.343 0.99
141_A 329_H 1.342 0.99
38_L 45_V 1.338 0.99
285_G 289_F 1.336 0.99
21_A 240_V 1.327 0.99
273_L 277_K 1.322 0.99
60_M 241_L 1.321 0.99
129_L 133_A 1.319 0.99
165_I 250_G 1.315 0.99
133_A 340_I 1.312 0.99
59_I 379_L 1.309 0.99
232_T 235_S 1.307 0.99
128_A 347_S 1.304 0.99
108_I 132_V 1.299 0.99
447_V 450_L 1.296 0.99
90_Y 94_W 1.295 0.99
25_T 244_I 1.295 0.99
136_A 337_A 1.293 0.99
341_A 345_I 1.29 0.99
70_E 82_R 1.286 0.99
83_Y 381_S 1.283 0.99
276_A 281_T 1.283 0.99
104_G 343_L 1.282 0.99
67_L 79_Y 1.268 0.99
111_I 292_L 1.261 0.99
310_L 319_L 1.261 0.99
135_V 299_C 1.256 0.99
195_F 202_F 1.254 0.99
132_V 340_I 1.252 0.99
73_T 224_G 1.251 0.99
52_A 209_V 1.247 0.99
12_L 17_I 1.244 0.98
9_Q 330_G 1.241 0.98
160_T 290_V 1.235 0.98
47_L 202_F 1.222 0.98
315_K 324_A 1.22 0.98
108_I 299_C 1.208 0.98
246_I 250_G 1.206 0.98
376_F 380_I 1.205 0.98
50_I 54_L 1.203 0.98
171_V 176_F 1.194 0.98
60_M 237_V 1.192 0.98
405_W 408_Y 1.185 0.98
326_V 330_G 1.185 0.98
8_L 311_Y 1.178 0.98
140_L 331_V 1.177 0.98
181_Q 186_S 1.167 0.97
143_V 304_Y 1.167 0.97
442_T 446_K 1.166 0.97
267_N 273_L 1.166 0.97
169_L 254_V 1.165 0.97
15_R 148_E 1.163 0.97
125_W 129_L 1.162 0.97
214_Q 371_G 1.159 0.97
32_M 110_A 1.156 0.97
223_A 233_L 1.153 0.97
393_I 396_H 1.146 0.97
390_K 396_H 1.143 0.97
325_K 333_V 1.143 0.97
58_F 406_A 1.133 0.97
46_L 253_F 1.131 0.97
72_V 78_V 1.129 0.97
78_V 82_R 1.127 0.97
63_M 76_F 1.121 0.97
74_G 77_A 1.121 0.97
433_V 436_I 1.12 0.96
436_I 440_A 1.12 0.96
94_W 441_V 1.118 0.96
387_R 390_K 1.118 0.96
440_A 444_I 1.116 0.96
53_G 213_Y 1.112 0.96
69_L 397_P 1.111 0.96
149_I 153_F 1.107 0.96
203_L 416_C 1.107 0.96
311_Y 326_V 1.107 0.96
97_W 310_L 1.107 0.96
334_A 337_A 1.106 0.96
217_E 221_I 1.106 0.96
136_A 140_L 1.104 0.96
402_L 405_W 1.104 0.96
250_G 254_V 1.098 0.96
69_L 396_H 1.097 0.96
230_Q 397_P 1.096 0.96
80_A 88_F 1.094 0.96
158_V 247_F 1.093 0.96
85_S 446_K 1.092 0.96
394_A 399_R 1.091 0.96
137_L 140_L 1.086 0.96
47_L 50_I 1.076 0.95
94_W 319_L 1.076 0.95
115_V 292_L 1.076 0.95
199_W 202_F 1.073 0.95
150_E 300_N 1.073 0.95
280_I 283_A 1.072 0.95
134_L 296_L 1.072 0.95
140_L 327_S 1.071 0.95
227_K 232_T 1.07 0.95
376_F 415_I 1.066 0.95
13_E 144_R 1.066 0.95
30_L 251_A 1.063 0.95
37_T 41_A 1.06 0.95
276_A 284_A 1.055 0.95
170_G 174_F 1.049 0.94
168_G 258_I 1.049 0.94
77_A 312_A 1.047 0.94
383_L 407_N 1.043 0.94
16_H 148_E 1.04 0.94
87_F 442_T 1.039 0.94
389_H 393_I 1.035 0.94
11_G 144_R 1.027 0.94
404_P 407_N 1.022 0.93
20_I 222_T 1.02 0.93
382_Q 386_R 1.017 0.93
162_I 166_V 1.016 0.93
15_R 19_L 1.016 0.93
126_I 130_I 1.014 0.93
383_L 387_R 1.013 0.93
133_A 137_L 1.013 0.93
303_M 307_G 1.012 0.93
379_L 411_M 1.01 0.93
8_L 225_E 1.009 0.93
135_V 300_N 1.008 0.93
148_E 152_W 1.008 0.93
61_R 238_G 1.005 0.93
327_S 330_G 1.003 0.93
15_R 151_F 1.002 0.92
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
4djkA20.96311000.324Contact Map0.593
3l1lA10.94581000.327Contact Map0.673
3giaA10.91971000.354Contact Map0.594
3dh4A40.937198.40.882Contact Map0.383
2xq2A10.937198.10.891Contact Map0.465
2jlnA10.889497.90.897Contact Map0.402
4m48A10.943694.80.927Contact Map0.394
2a65A10.924192.70.934Contact Map0.397
2wswA10.765750.90.956Contact Map0.309
2kluA10.097614.30.967Contact Map0.192
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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