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OPENSEQ.org
PLAP - Low-affinity putrescine importer PlaP
UniProt: P0AA47 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG11896
Length: 452 (427)
Sequences: 10800
Seq/Len: 25.29
PLAP
Paralog alert: 0.81 [within 20: 0.39] - ratio of genomes with paralogs
Cluster includes: ADIC ANSP ARCD AROP CADB CYCA FRLA GABP GADC LYSP MMUP PHEP PLAP POTE PROY PUUP YBAT YCAM YGJI YIFK YJEH YJEM
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
324_S 338_M 3.823 1.00
142_T 344_G 3.513 1.00
371_T 405_A 3.304 1.00
174_V 255_F 3.141 1.00
316_G 327_G 3.051 1.00
19_T 228_K 3.002 1.00
328_Y 338_M 2.995 1.00
59_L 246_G 2.922 1.00
229_D 232_R 2.898 1.00
232_R 236_R 2.842 1.00
135_V 351_I 2.826 1.00
374_N 404_G 2.506 1.00
341_I 345_A 2.412 1.00
284_A 288_G 2.37 1.00
402_M 406_L 2.367 1.00
330_H 333_W 2.366 1.00
319_G 323_K 2.331 1.00
172_G 176_Y 2.318 1.00
99_G 315_M 2.281 1.00
227_T 233_V 2.271 1.00
324_S 328_Y 2.27 1.00
313_Y 317_R 2.264 1.00
20_L 240_L 2.253 1.00
24_V 240_L 2.223 1.00
330_H 337_A 2.201 1.00
20_L 233_V 2.142 1.00
68_S 374_N 2.115 1.00
50_H 203_M 2.06 1.00
83_Y 318_D 2.028 1.00
326_F 342_L 2.025 1.00
87_Q 92_P 2.002 1.00
60_I 403_C 1.98 1.00
225_E 317_R 1.978 1.00
87_Q 95_G 1.963 1.00
265_R 279_Y 1.961 1.00
90_I 379_S 1.957 1.00
14_L 329_V 1.954 1.00
90_I 375_L 1.901 1.00
20_L 24_V 1.89 1.00
146_L 337_A 1.872 1.00
19_T 22_P 1.87 1.00
23_V 227_T 1.858 1.00
142_T 340_I 1.838 1.00
143_A 147_R 1.838 1.00
236_R 240_L 1.815 1.00
406_L 410_A 1.813 1.00
399_F 403_C 1.808 1.00
60_I 64_F 1.806 1.00
150_K 154_N 1.802 1.00
103_L 343_V 1.787 1.00
226_E 310_R 1.773 1.00
375_L 405_A 1.733 1.00
146_L 341_I 1.73 1.00
161_V 165_V 1.693 1.00
71_K 381_F 1.686 1.00
54_A 206_G 1.678 1.00
396_Q 400_L 1.673 1.00
243_L 247_M 1.662 1.00
43_V 51_V 1.662 1.00
239_F 243_L 1.65 1.00
229_D 233_V 1.638 1.00
86_A 98_V 1.636 1.00
98_V 372_F 1.618 1.00
24_V 244_I 1.614 1.00
164_V 168_A 1.612 1.00
100_W 430_G 1.602 1.00
379_S 383_I 1.6 1.00
56_A 250_I 1.588 1.00
61_A 407_T 1.581 1.00
229_D 236_R 1.571 1.00
22_P 157_T 1.571 1.00
424_L 428_A 1.557 1.00
287_V 291_I 1.557 1.00
18_L 22_P 1.548 1.00
275_E 279_Y 1.527 1.00
379_S 382_W 1.521 1.00
308_V 343_V 1.515 1.00
230_A 235_P 1.514 1.00
275_E 278_L 1.514 1.00
171_L 251_F 1.505 1.00
86_A 372_F 1.493 1.00
378_I 402_M 1.489 1.00
312_M 339_N 1.478 1.00
139_A 344_G 1.439 1.00
223_L 306_A 1.438 1.00
405_A 409_G 1.437 1.00
138_V 347_A 1.433 1.00
392_K 395_F 1.429 1.00
91_S 94_V 1.427 1.00
165_V 169_V 1.422 1.00
261_P 264_S 1.416 1.00
432_I 435_A 1.415 1.00
44_S 51_V 1.413 1.00
309_A 340_I 1.412 1.00
68_S 401_P 1.401 0.99
338_M 342_L 1.397 0.99
114_I 301_G 1.395 0.99
114_I 138_V 1.388 0.99
90_I 94_V 1.371 0.99
269_P 275_E 1.368 0.99
145_N 302_M 1.364 0.99
142_T 341_I 1.363 0.99
174_V 259_Y 1.36 0.99
76_Y 88_K 1.357 0.99
28_L 244_I 1.348 0.99
383_I 387_R 1.344 0.99
20_L 236_R 1.344 0.99
333_W 337_A 1.341 0.99
110_P 347_A 1.34 0.99
66_A 242_A 1.339 0.99
175_V 255_F 1.338 0.99
375_L 379_S 1.337 0.99
117_A 294_T 1.333 0.99
421_V 425_I 1.331 0.99
121_F 133_F 1.331 0.99
31_M 245_G 1.324 0.99
147_R 333_W 1.321 0.99
323_K 328_Y 1.316 0.99
141_M 301_G 1.314 0.99
18_L 153_A 1.313 0.99
89_S 376_S 1.312 0.99
278_L 286_Q 1.308 0.99
65_T 374_N 1.307 0.99
386_K 390_T 1.306 0.99
96_F 100_W 1.301 0.99
38_D 116_L 1.297 0.99
64_F 403_C 1.29 0.99
323_K 327_G 1.286 0.99
377_V 401_P 1.282 0.99
135_V 348_L 1.28 0.99
278_L 283_K 1.275 0.99
330_H 335_T 1.268 0.99
134_V 351_I 1.266 0.99
233_V 236_R 1.265 0.99
27_G 241_T 1.258 0.99
135_V 139_A 1.239 0.98
261_P 265_R 1.234 0.98
312_M 321_F 1.229 0.98
139_A 348_L 1.222 0.98
166_L 292_F 1.222 0.98
138_V 344_G 1.216 0.98
329_V 334_R 1.215 0.98
142_T 146_L 1.208 0.98
73_V 85_Y 1.207 0.98
282_G 285_F 1.207 0.98
66_A 238_I 1.206 0.98
15_R 334_R 1.206 0.98
58_A 210_L 1.205 0.98
52_P 254_Y 1.2 0.98
281_A 285_F 1.193 0.98
328_Y 337_A 1.191 0.98
379_S 384_R 1.187 0.98
317_R 327_G 1.184 0.98
79_A 225_E 1.184 0.98
100_W 321_F 1.184 0.98
149_L 306_A 1.182 0.98
18_L 23_V 1.178 0.98
215_T 366_A 1.178 0.98
371_T 375_L 1.175 0.98
74_R 391_L 1.172 0.98
371_T 409_G 1.171 0.98
398_L 402_M 1.151 0.97
78_S 84_T 1.148 0.97
53_T 203_M 1.143 0.97
84_T 88_K 1.142 0.97
170_I 174_V 1.134 0.97
228_K 233_V 1.126 0.97
17_T 150_K 1.12 0.96
313_Y 329_V 1.12 0.96
21_V 25_M 1.116 0.96
83_Y 314_V 1.112 0.96
396_Q 399_F 1.108 0.96
56_A 60_I 1.105 0.96
131_W 135_V 1.103 0.96
247_M 251_F 1.101 0.96
69_Y 82_A 1.096 0.96
21_V 154_N 1.096 0.96
80_G 83_Y 1.094 0.96
86_A 94_V 1.093 0.96
14_L 313_Y 1.091 0.96
338_M 341_I 1.089 0.96
143_A 146_L 1.086 0.96
252_A 273_Q 1.077 0.95
422_L 425_I 1.068 0.95
64_F 400_L 1.061 0.95
146_L 330_H 1.06 0.95
22_P 154_N 1.055 0.95
164_V 248_I 1.052 0.95
155_F 159_I 1.052 0.95
72_L 377_V 1.051 0.94
86_A 376_S 1.045 0.94
425_I 429_I 1.041 0.94
59_L 214_F 1.041 0.94
23_V 237_A 1.041 0.94
417_E 421_V 1.039 0.94
251_F 255_F 1.034 0.94
406_L 409_G 1.032 0.94
105_D 366_A 1.031 0.94
259_Y 276_I 1.03 0.94
364_F 423_G 1.029 0.94
179_F 258_L 1.023 0.93
345_A 349_L 1.022 0.93
146_L 335_T 1.021 0.93
53_T 56_A 1.021 0.93
224_S 234_I 1.02 0.93
27_G 220_I 1.019 0.93
112_I 212_F 1.017 0.93
384_R 387_R 1.017 0.93
305_H 309_A 1.014 0.93
398_L 401_P 1.012 0.93
144_F 155_F 1.012 0.93
71_K 74_R 1.008 0.93
378_I 401_P 1.006 0.93
22_P 153_A 1.002 0.92
25_M 157_T 1 0.92
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
3l1lA10.94911000.357Contact Map0.703
4djkA20.96461000.357Contact Map0.595
3giaA10.9271000.385Contact Map0.622
2jlnA10.909398.60.879Contact Map0.42
3dh4A40.951398.30.889Contact Map0.417
2xq2A10.951397.90.9Contact Map0.498
4m48A10.938196.30.922Contact Map0.426
2a65A10.918192.50.936Contact Map0.478
2wswA10.807570.30.952Contact Map0.288
3j1zP20.276521.20.965Contact Map0.278
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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