May 4, 2021 - We are working on upgrading the webserver, some pages may not work.
OPENSEQ.org
DGAL - D-galactose-binding periplasmic protein
UniProt: P0AEE5 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG10593
Length: 332 (283)
Sequences: 13003
Seq/Len: 45.95
DGAL
Paralog alert: 0.90 [within 20: 0.48] - ratio of genomes with paralogs
Cluster includes: ALSB ARAF ASCG CRA CYTR DGAL EBGR GALR GALS GNTR IDNR LACI LSRB MALI PURR RBSB RBSR TORT TRER XYLF YCJW YPHF YTFQ
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
136_K 187_K 3.709 1.00
163_Q 228_E 2.932 1.00
85_A 292_A 2.919 1.00
142_Q 256_F 2.853 1.00
260_A 278_L 2.582 1.00
182_T 186_I 2.554 1.00
245_L 253_I 2.505 1.00
144_D 148_K 2.441 1.00
46_A 285_A 2.351 1.00
140_I 191_D 2.306 1.00
212_K 247_A 2.301 1.00
140_I 187_K 2.298 1.00
50_D 290_D 2.25 1.00
129_Y 295_L 2.233 1.00
50_D 53_A 2.206 1.00
287_A 290_D 2.195 1.00
139_G 143_G 2.125 1.00
51_A 289_F 2.075 1.00
140_I 188_E 2.05 1.00
138_S 258_V 2.017 1.00
215_M 230_V 1.976 1.00
146_I 229_V 1.962 1.00
294_N 299_K 1.962 1.00
137_E 141_I 1.934 1.00
220_S 248_H 1.917 1.00
50_D 286_K 1.906 1.00
216_D 244_A 1.902 1.00
144_D 188_E 1.9 1.00
216_D 248_H 1.899 1.00
49_Q 282_N 1.881 1.00
147_A 192_K 1.869 1.00
136_K 183_T 1.825 1.00
140_I 144_D 1.809 1.00
139_G 231_I 1.793 1.00
145_L 149_H 1.785 1.00
163_Q 254_P 1.772 1.00
211_A 240_G 1.768 1.00
212_K 244_A 1.739 1.00
278_L 319_V 1.739 1.00
280_D 283_N 1.709 1.00
143_G 185_V 1.696 1.00
143_G 147_A 1.676 1.00
29_G 83_V 1.671 1.00
137_E 140_I 1.671 1.00
183_T 187_K 1.667 1.00
29_G 78_L 1.655 1.00
144_D 192_K 1.648 1.00
147_A 188_E 1.643 1.00
50_D 289_F 1.616 1.00
113_F 288_T 1.612 1.00
232_A 241_A 1.578 1.00
169_L 241_A 1.575 1.00
283_N 286_K 1.574 1.00
215_M 241_A 1.572 1.00
134_D 137_E 1.554 1.00
167_V 227_I 1.512 1.00
244_A 248_H 1.511 1.00
290_D 294_N 1.498 1.00
246_K 272_A 1.495 1.00
293_K 297_D 1.486 1.00
101_E 105_G 1.484 1.00
216_D 247_A 1.483 1.00
145_L 148_K 1.482 1.00
46_A 282_N 1.476 1.00
246_K 270_S 1.475 1.00
28_I 51_A 1.475 1.00
30_V 47_I 1.468 1.00
139_G 184_Y 1.464 1.00
184_Y 188_E 1.455 1.00
55_P 293_K 1.446 1.00
180_A 183_T 1.438 1.00
144_D 191_D 1.433 1.00
260_A 323_K 1.426 1.00
283_N 287_A 1.425 1.00
51_A 59_L 1.424 1.00
244_A 247_A 1.419 1.00
168_L 233_N 1.413 1.00
205_M 210_Q 1.408 1.00
245_L 250_K 1.402 0.99
143_G 184_Y 1.388 0.99
215_M 245_L 1.385 0.99
143_G 188_E 1.371 0.99
141_I 318_Y 1.366 0.99
167_V 218_W 1.365 0.99
160_K 228_E 1.364 0.99
186_I 190_N 1.359 0.99
242_V 255_V 1.351 0.99
62_N 78_L 1.34 0.99
209_A 213_D 1.336 0.99
240_G 244_A 1.33 0.99
48_E 52_K 1.322 0.99
138_S 318_Y 1.304 0.99
27_R 58_Q 1.289 0.99
50_D 55_P 1.28 0.99
30_V 59_L 1.272 0.99
235_D 276_T 1.266 0.99
280_D 316_V 1.258 0.99
212_K 243_E 1.242 0.98
145_L 322_D 1.239 0.98
185_V 189_L 1.237 0.98
147_A 194_I 1.228 0.98
149_H 254_P 1.228 0.98
26_T 57_V 1.225 0.98
212_K 216_D 1.224 0.98
188_E 191_D 1.216 0.98
165_Q 226_K 1.216 0.98
62_N 74_Q 1.209 0.98
136_K 140_I 1.208 0.98
187_K 191_D 1.203 0.98
47_I 87_A 1.202 0.98
87_A 288_T 1.201 0.98
76_D 102_K 1.2 0.98
213_D 217_A 1.19 0.98
26_T 84_K 1.186 0.98
141_I 144_D 1.183 0.98
257_G 276_T 1.178 0.98
136_K 184_Y 1.162 0.97
235_D 257_G 1.159 0.97
149_H 322_D 1.157 0.97
47_I 289_F 1.151 0.97
136_K 180_A 1.137 0.97
230_V 241_A 1.134 0.97
245_L 255_V 1.114 0.96
197_E 200_Q 1.112 0.96
202_D 217_A 1.102 0.96
207_D 210_Q 1.101 0.96
286_K 290_D 1.094 0.96
166_F 189_L 1.091 0.96
211_A 215_M 1.087 0.96
166_F 229_V 1.082 0.96
256_F 275_G 1.081 0.95
70_K 73_D 1.08 0.95
215_M 219_L 1.078 0.95
232_A 238_A 1.078 0.95
210_Q 213_D 1.077 0.95
235_D 238_A 1.066 0.95
168_L 182_T 1.065 0.95
285_A 289_F 1.06 0.95
287_A 291_L 1.054 0.95
269_K 327_A 1.054 0.95
163_Q 229_V 1.051 0.94
142_Q 277_V 1.051 0.94
142_Q 231_I 1.051 0.94
133_T 279_N 1.051 0.94
185_V 231_I 1.05 0.94
47_I 285_A 1.048 0.94
165_Q 228_E 1.047 0.94
282_N 286_K 1.035 0.94
187_K 190_N 1.035 0.94
148_K 192_K 1.035 0.94
272_A 324_D 1.029 0.94
232_A 237_M 1.02 0.93
228_E 254_P 1.018 0.93
224_A 227_I 1.011 0.93
287_A 314_V 1.009 0.93
210_Q 214_K 1.006 0.93
165_Q 197_E 1.005 0.93
204_A 237_M 1.001 0.92
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
4kzkA10.93981000.292Contact Map0.64
2driA10.81631000.295Contact Map0.709
4kvfA10.90061000.298Contact Map0.607
3d02A10.87651000.303Contact Map0.596
2fvyA10.93071000.305Contact Map0.661
4irxA20.86451000.306Contact Map0.698
3g1wA20.87951000.315Contact Map0.741
2hsgA10.88251000.319Contact Map0.762
2rgyA10.84041000.319Contact Map0.743
1tjyA10.87951000.319Contact Map0.615
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
Page generated in 0.055 seconds.