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OPENSEQ.org
ALSB - D-allose-binding periplasmic protein
UniProt: P39265 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG12458
Length: 311 (274)
Sequences: 15202
Seq/Len: 55.48
ALSB
Paralog alert: 0.90 [within 20: 0.53] - ratio of genomes with paralogs
Cluster includes: ALSB ARAF ASCG CRA CYTR DGAL EBGR GALR GALS GNTR IDNR LACI LSRB MALI PURR RBSB RBSR TORT TRER XYLF YCJW YPHF YTFQ
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
138_V 180_E 3.714 1.00
144_G 247_V 2.97 1.00
85_G 283_M 2.782 1.00
154_A 218_K 2.769 1.00
175_R 179_T 2.677 1.00
251_G 269_A 2.636 1.00
235_V 244_V 2.362 1.00
146_S 150_D 2.332 1.00
49_E 281_K 2.277 1.00
142_A 184_K 2.257 1.00
142_A 180_E 2.242 1.00
204_L 237_N 2.215 1.00
45_G 276_G 2.202 1.00
49_E 52_T 2.2 1.00
278_T 281_K 2.195 1.00
131_A 286_A 2.097 1.00
142_A 181_A 2.087 1.00
141_G 145_A 2.073 1.00
149_I 185_A 2.065 1.00
140_V 249_T 2.041 1.00
146_S 181_A 2.011 1.00
50_A 280_L 1.992 1.00
139_A 143_K 1.99 1.00
154_A 245_L 1.981 1.00
49_E 277_A 1.962 1.00
208_T 238_A 1.956 1.00
148_I 219_A 1.945 1.00
138_V 176_N 1.882 1.00
212_Q 238_A 1.878 1.00
48_D 273_A 1.877 1.00
208_T 234_A 1.873 1.00
285_D 290_G 1.865 1.00
207_A 220_I 1.848 1.00
142_A 146_S 1.847 1.00
240_K 243_K 1.838 1.00
147_F 151_K 1.813 1.00
141_G 221_Y 1.787 1.00
145_A 178_A 1.744 1.00
146_S 185_A 1.733 1.00
203_A 230_G 1.725 1.00
271_N 274_D 1.702 1.00
204_L 234_A 1.688 1.00
139_A 142_A 1.68 1.00
149_I 181_A 1.668 1.00
28_A 83_Y 1.666 1.00
145_A 149_I 1.655 1.00
176_N 180_E 1.65 1.00
207_A 231_V 1.637 1.00
113_L 279_G 1.631 1.00
269_A 307_I 1.614 1.00
49_E 280_L 1.598 1.00
160_A 217_I 1.598 1.00
274_D 277_A 1.587 1.00
158_E 216_N 1.574 1.00
222_C 231_V 1.553 1.00
136_D 139_A 1.541 1.00
53_L 284_V 1.535 1.00
162_I 231_V 1.535 1.00
155_E 218_K 1.531 1.00
45_G 273_A 1.519 1.00
281_K 285_D 1.499 1.00
27_Y 50_A 1.492 1.00
234_A 238_A 1.486 1.00
161_I 223_A 1.479 1.00
177_G 181_A 1.478 1.00
50_A 57_V 1.474 1.00
146_S 184_K 1.472 1.00
147_F 150_D 1.464 1.00
28_A 78_L 1.459 1.00
208_T 237_N 1.452 1.00
29_V 46_I 1.446 1.00
274_D 278_T 1.44 1.00
234_A 237_N 1.434 1.00
25_A 55_V 1.422 1.00
101_A 105_K 1.419 1.00
207_A 235_V 1.406 0.99
284_V 288_K 1.402 0.99
173_A 176_N 1.4 0.99
235_V 240_K 1.392 0.99
49_E 53_L 1.391 0.99
143_K 306_S 1.369 0.99
145_A 177_G 1.351 0.99
232_A 246_V 1.347 0.99
145_A 181_A 1.342 0.99
181_A 184_K 1.333 0.99
201_I 205_D 1.33 0.99
141_G 177_G 1.33 0.99
197_D 202_K 1.33 0.99
247_V 266_A 1.327 0.99
160_A 210_V 1.326 0.99
25_A 84_K 1.325 0.99
158_E 189_K 1.316 0.99
179_T 183_K 1.315 0.99
236_A 263_Q 1.307 0.99
225_D 267_T 1.26 0.99
230_G 234_A 1.252 0.99
47_E 51_K 1.252 0.99
204_L 208_T 1.251 0.99
178_A 182_F 1.24 0.98
62_S 78_L 1.237 0.98
158_E 218_K 1.234 0.98
46_I 87_A 1.233 0.98
138_V 142_A 1.232 0.98
204_L 233_Q 1.232 0.98
87_A 279_G 1.207 0.98
149_I 188_I 1.191 0.98
46_I 280_L 1.19 0.98
205_D 209_N 1.186 0.98
29_V 57_V 1.185 0.98
76_E 102_R 1.184 0.98
138_V 177_G 1.184 0.98
248_G 267_T 1.184 0.98
214_N 217_I 1.176 0.98
143_K 146_S 1.171 0.98
161_I 175_R 1.156 0.97
140_V 306_S 1.151 0.97
159_V 182_F 1.15 0.97
26_E 56_S 1.15 0.97
220_I 231_V 1.133 0.97
271_N 304_V 1.118 0.96
159_V 219_A 1.116 0.96
178_A 221_Y 1.113 0.96
222_C 228_A 1.113 0.96
194_Q 209_N 1.112 0.96
138_V 173_A 1.107 0.96
225_D 248_G 1.102 0.96
70_S 73_Q 1.099 0.96
207_A 211_L 1.091 0.96
150_D 185_A 1.09 0.96
62_S 74_L 1.086 0.96
151_K 245_L 1.086 0.96
236_A 261_A 1.077 0.95
235_V 246_V 1.074 0.95
202_K 205_D 1.068 0.95
144_G 268_V 1.062 0.95
278_T 282_L 1.06 0.95
276_G 280_L 1.049 0.94
26_E 58_D 1.047 0.94
277_A 281_K 1.043 0.94
176_N 179_T 1.034 0.94
182_F 188_I 1.033 0.94
53_L 281_K 1.022 0.93
225_D 228_A 1.021 0.93
144_G 221_Y 1.021 0.93
199_D 202_K 1.02 0.93
135_T 270_Q 1.018 0.93
46_I 276_G 1.014 0.93
273_A 277_A 1.004 0.93
222_C 227_M 1.002 0.92
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
2driA10.86821000.202Contact Map0.745
4kvfA10.9261000.234Contact Map0.634
3d02A10.90681000.244Contact Map0.615
2rjoA10.9261000.245Contact Map0.681
2rgyA10.8651000.245Contact Map0.749
3ksmA20.87781000.247Contact Map0.777
3l6uA20.89071000.249Contact Map0.738
2iksA20.90031000.249Contact Map0.73
4irxA20.88751000.252Contact Map0.726
4kzkA10.94861000.252Contact Map0.65
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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