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OPENSEQ.org
NAC - Nitrogen assimilation regulatory protein nac
UniProt: Q47005 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG14265
Length: 305 (292)
Sequences: 37290
Seq/Len: 127.71
NAC
Paralog alert: 0.88 [within 20: 0.50] - ratio of genomes with paralogs
Cluster includes: AAER ABGR ALLS CBL CYNR CYSB DMLR DSDC GCVA HCAR HDFR ICIA ILVY LEUO LRHA LYSR METR NAC NHAR OXYR PERR TDCA TTDR XAPR YAFC YAHB YBDO YBEF YBHD YCAN YCJZ YDCI YDHB YEEY YEIE YFER YFIE YGFI YHAJ YHJC YIAU YIDZ YJIE YNEJ YNFL
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
70_A 74_Q 3.722 1.00
15_I 24_V 3.415 1.00
159_Q 288_M 2.591 1.00
229_I 236_A 2.516 1.00
96_G 142_L 2.436 1.00
20_Q 23_E 2.365 1.00
67_H 81_A 2.354 1.00
165_D 240_E 2.229 1.00
11_K 24_V 2.225 1.00
180_D 183_A 2.212 1.00
14_D 69_R 2.211 1.00
94_S 124_Y 2.161 1.00
92_Q 122_V 2.157 1.00
71_I 75_C 2.15 1.00
92_Q 124_Y 2.123 1.00
160_A 163_K 2.109 1.00
13_V 57_P 2.068 1.00
67_H 74_Q 2.062 1.00
18_L 36_V 2.024 1.00
163_K 265_S 2.003 1.00
8_Y 35_Q 1.981 1.00
113_A 290_V 1.974 1.00
226_T 246_L 1.973 1.00
10_V 65_Y 1.972 1.00
45_Q 60_A 1.963 1.00
65_Y 69_R 1.938 1.00
11_K 15_I 1.936 1.00
10_V 69_R 1.893 1.00
10_V 14_D 1.879 1.00
11_K 14_D 1.876 1.00
113_A 286_L 1.871 1.00
174_C 187_M 1.851 1.00
8_Y 32_L 1.85 1.00
64_L 78_A 1.822 1.00
190_F 234_G 1.813 1.00
49_I 56_T 1.804 1.00
114_V 121_I 1.804 1.00
160_A 265_S 1.794 1.00
167_F 240_E 1.792 1.00
230_A 246_L 1.788 1.00
13_V 65_Y 1.755 1.00
133_L 145_A 1.711 1.00
230_A 251_N 1.711 1.00
135_E 139_N 1.682 1.00
59_D 62_K 1.681 1.00
9_F 65_Y 1.669 1.00
63_I 81_A 1.667 1.00
14_D 24_V 1.665 1.00
10_V 68_A 1.653 1.00
188_N 216_I 1.643 1.00
28_A 31_A 1.641 1.00
8_Y 27_I 1.632 1.00
19_T 29_Q 1.626 1.00
114_V 283_V 1.608 1.00
15_I 20_Q 1.604 1.00
17_S 20_Q 1.59 1.00
88_A 120_E 1.589 1.00
163_K 263_S 1.586 1.00
10_V 72_L 1.569 1.00
136_K 141_Q 1.493 1.00
158_S 267_S 1.49 1.00
67_H 77_Q 1.481 1.00
71_I 78_A 1.479 1.00
190_F 225_L 1.472 1.00
5_R 35_Q 1.452 1.00
5_R 38_T 1.449 1.00
11_K 25_L 1.432 1.00
46_Q 49_I 1.425 1.00
187_M 235_V 1.418 1.00
144_M 280_A 1.408 1.00
5_R 42_E 1.405 0.99
113_A 117_E 1.405 0.99
62_K 66_T 1.404 0.99
2_N 5_R 1.4 0.99
199_R 203_D 1.391 0.99
64_L 79_Q 1.387 0.99
3_F 75_C 1.377 0.99
157_S 272_A 1.359 0.99
90_S 120_E 1.354 0.99
170_G 255_S 1.346 0.99
128_N 132_V 1.345 0.99
225_L 236_A 1.339 0.99
157_S 270_L 1.333 0.99
67_H 70_A 1.327 0.99
282_A 285_E 1.323 0.99
268_L 284_K 1.321 0.99
170_G 235_V 1.321 0.99
37_A 41_G 1.314 0.99
113_A 116_A 1.31 0.99
136_K 142_L 1.304 0.99
246_L 251_N 1.279 0.99
112_Q 116_A 1.276 0.99
276_L 280_A 1.274 0.99
34_Q 37_A 1.267 0.99
159_Q 270_L 1.247 0.99
114_V 286_L 1.246 0.98
284_K 288_M 1.243 0.98
9_F 13_V 1.242 0.98
115_R 188_N 1.24 0.98
42_E 79_Q 1.24 0.98
219_I 225_L 1.235 0.98
93_V 283_V 1.23 0.98
12_I 36_V 1.228 0.98
244_R 248_G 1.224 0.98
67_H 78_A 1.22 0.98
227_A 230_A 1.212 0.98
9_F 48_L 1.212 0.98
66_T 70_A 1.209 0.98
94_S 126_H 1.203 0.98
117_E 286_L 1.198 0.98
57_P 61_G 1.195 0.98
27_I 31_A 1.194 0.98
51_T 56_T 1.193 0.98
63_I 66_T 1.19 0.98
144_M 268_L 1.188 0.98
33_S 37_A 1.187 0.98
167_F 254_M 1.184 0.98
74_Q 77_Q 1.178 0.98
179_V 183_A 1.17 0.97
9_F 47_L 1.168 0.97
12_I 21_A 1.167 0.97
18_L 55_V 1.164 0.97
13_V 48_L 1.162 0.97
138_I 155_G 1.161 0.97
240_E 244_R 1.157 0.97
206_F 211_L 1.153 0.97
172_Q 251_N 1.149 0.97
170_G 174_C 1.143 0.97
115_R 216_I 1.137 0.97
270_L 284_K 1.135 0.97
35_Q 38_T 1.134 0.97
7_K 76_E 1.134 0.97
147_I 269_N 1.125 0.97
11_K 21_A 1.115 0.96
164_E 241_S 1.109 0.96
285_E 288_M 1.109 0.96
96_G 147_I 1.102 0.96
34_Q 38_T 1.102 0.96
63_I 67_H 1.096 0.96
117_E 289_S 1.09 0.96
4_R 7_K 1.085 0.96
112_Q 231_S 1.084 0.96
177_Q 253_W 1.083 0.96
114_V 118_F 1.082 0.96
226_T 230_A 1.081 0.95
6_L 68_A 1.078 0.95
281_Q 285_E 1.077 0.95
245_S 249_A 1.075 0.95
160_A 267_S 1.068 0.95
227_A 231_S 1.065 0.95
48_L 55_V 1.06 0.95
118_F 282_A 1.057 0.95
158_S 269_N 1.056 0.95
137_L 145_A 1.055 0.95
168_L 235_V 1.055 0.95
171_T 232_G 1.052 0.95
71_I 74_Q 1.051 0.94
27_I 32_L 1.048 0.94
271_P 274_A 1.047 0.94
149_E 267_S 1.046 0.94
188_N 214_K 1.045 0.94
76_E 80_L 1.035 0.94
219_I 228_A 1.033 0.94
70_A 77_Q 1.027 0.94
13_V 61_G 1.025 0.93
64_L 68_A 1.024 0.93
169_V 243_A 1.012 0.93
12_I 48_L 1.001 0.92
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
1uthA10.9771000.143Contact Map0.772
2esnA40.96391000.144Contact Map0.769
1ixcA20.94431000.147Contact Map0.775
3fxqA20.9771000.148Contact Map0.798
3fzvA40.97051000.149Contact Map0.717
2h98A20.98031000.151Contact Map0.767
1al3A10.99021000.162Contact Map0.749
2h9bA20.98031000.166Contact Map0.769
3szpA20.9411000.17Contact Map0.762
3hhgA80.9771000.183Contact Map0.929
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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