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OPENSEQ.org
FRMA - S-(hydroxymethyl)glutathione dehydrogenase
UniProt: P25437 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG50010
Length: 369 (355)
Sequences: 21949
Seq/Len: 61.83
FRMA
Paralog alert: 0.83 [within 20: 0.37] - ratio of genomes with paralogs
Cluster includes: ACUI ADHP CURA FRMA GATD IDND QOR1 RSPB TDH YAHK YBDR YCJQ YDJJ YDJL YGGP YJGB YJJN YPHC
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
349_E 352_D 3.653 1.00
35_T 161_H 3.314 1.00
33_K 69_V 3.227 1.00
30_V 152_L 3.172 1.00
35_T 67_V 3.01 1.00
207_R 233_T 3.007 1.00
31_L 69_V 2.934 1.00
160_N 163_H 2.857 1.00
82_D 154_K 2.853 1.00
225_D 229_R 2.707 1.00
345_M 356_L 2.682 1.00
28_G 74_G 2.629 1.00
189_S 213_R 2.622 1.00
248_V 251_D 2.606 1.00
224_F 228_R 2.587 1.00
79_K 82_D 2.566 1.00
9_F 18_V 2.564 1.00
162_E 344_T 2.53 1.00
27_K 73_E 2.522 1.00
33_K 67_V 2.507 1.00
213_R 234_D 2.473 1.00
83_H 155_I 2.456 1.00
33_K 144_Y 2.43 1.00
346_S 349_E 2.355 1.00
190_V 206_A 2.343 1.00
77_S 149_E 2.306 1.00
27_K 74_G 2.266 1.00
328_E 332_K 2.242 1.00
221_P 225_D 2.232 1.00
22_V 144_Y 2.221 1.00
191_A 257_I 2.171 1.00
185_Q 188_D 2.164 1.00
83_H 161_H 2.147 1.00
188_D 283_Q 2.14 1.00
64_G 140_T 2.116 1.00
24_P 70_E 2.108 1.00
203_V 232_A 2.092 1.00
221_P 237_N 2.07 1.00
352_D 355_D 2.055 1.00
277_A 284_S 2.042 1.00
345_M 353_A 2.038 1.00
355_D 359_E 2.035 1.00
65_A 161_H 2.02 1.00
158_E 331_M 2.015 1.00
343_H 356_L 2.015 1.00
7_V 18_V 2.007 1.00
191_A 217_I 2.004 1.00
225_D 228_R 1.985 1.00
26_K 29_E 1.975 1.00
31_L 146_V 1.948 1.00
9_F 16_E 1.912 1.00
155_I 164_V 1.904 1.00
72_G 75_V 1.883 1.00
69_V 144_Y 1.883 1.00
83_H 157_P 1.856 1.00
218_D 224_F 1.85 1.00
171_V 201_A 1.841 1.00
224_F 235_C 1.834 1.00
237_N 240_D 1.795 1.00
344_T 368_R 1.785 1.00
78_V 154_K 1.779 1.00
356_L 361_K 1.766 1.00
4_R 19_E 1.754 1.00
71_V 80_P 1.754 1.00
163_H 338_E 1.753 1.00
70_E 80_P 1.741 1.00
258_D 278_H 1.728 1.00
29_E 148_A 1.725 1.00
48_S 358_H 1.715 1.00
352_D 356_L 1.701 1.00
244_P 247_D 1.697 1.00
64_G 86_P 1.692 1.00
155_I 161_H 1.668 1.00
226_L 229_R 1.661 1.00
215_I 257_I 1.654 1.00
203_V 216_A 1.648 1.00
68_V 78_V 1.647 1.00
182_A 259_H 1.642 1.00
179_H 209_A 1.633 1.00
260_T 273_A 1.633 1.00
25_P 146_V 1.628 1.00
283_Q 308_V 1.624 1.00
221_P 240_D 1.616 1.00
193_F 273_A 1.615 1.00
195_L 216_A 1.582 1.00
277_A 282_G 1.578 1.00
29_E 146_V 1.572 1.00
234_D 252_I 1.557 1.00
95_C 98_C 1.552 1.00
34_V 64_G 1.539 1.00
67_V 83_H 1.538 1.00
28_G 148_A 1.531 1.00
78_V 82_D 1.524 1.00
31_L 144_Y 1.521 1.00
283_Q 310_K 1.514 1.00
4_R 143_E 1.495 1.00
30_V 78_V 1.493 1.00
36_H 162_E 1.489 1.00
141_F 354_F 1.448 1.00
67_V 161_H 1.445 1.00
25_P 29_E 1.437 1.00
31_L 70_E 1.426 1.00
78_V 152_L 1.424 1.00
208_Q 334_D 1.423 1.00
277_A 307_R 1.42 1.00
5_A 20_I 1.399 0.99
92_C 95_C 1.398 0.99
6_A 350_I 1.388 0.99
36_H 368_R 1.382 0.99
25_P 31_L 1.379 0.99
351_N 355_D 1.379 0.99
202_V 261_F 1.373 0.99
216_A 232_A 1.366 0.99
213_R 233_T 1.362 0.99
32_I 66_G 1.353 0.99
189_S 215_I 1.35 0.99
184_V 211_A 1.338 0.99
260_T 277_A 1.329 0.99
98_C 106_C 1.314 0.99
35_T 83_H 1.311 0.99
95_C 106_C 1.305 0.99
247_D 251_D 1.305 0.99
328_E 331_M 1.302 0.99
6_A 17_I 1.301 0.99
219_T 238_P 1.295 0.99
47_L 354_F 1.284 0.99
4_R 348_D 1.272 0.99
342_T 356_L 1.262 0.99
193_F 217_I 1.252 0.99
10_A 13_K 1.247 0.99
36_H 161_H 1.246 0.98
39_V 353_A 1.242 0.98
243_K 248_V 1.24 0.98
28_G 149_E 1.236 0.98
228_R 235_C 1.231 0.98
147_V 152_L 1.223 0.98
40_C 62_H 1.216 0.98
22_V 31_L 1.213 0.98
248_V 252_I 1.203 0.98
189_S 258_D 1.2 0.98
221_P 224_F 1.183 0.98
192_V 202_V 1.171 0.98
182_A 285_V 1.171 0.98
71_V 75_V 1.163 0.97
92_C 98_C 1.146 0.97
17_I 348_D 1.145 0.97
162_E 368_R 1.145 0.97
219_T 239_N 1.143 0.97
262_E 265_G 1.115 0.96
228_R 234_D 1.113 0.96
38_G 63_E 1.109 0.96
34_V 145_T 1.109 0.96
167_L 330_A 1.106 0.96
159_A 164_V 1.102 0.96
40_C 63_E 1.099 0.96
224_F 237_N 1.093 0.96
229_R 339_P 1.092 0.96
79_K 154_K 1.088 0.96
35_T 65_A 1.083 0.96
325_G 328_E 1.081 0.95
32_I 84_V 1.08 0.95
329_D 334_D 1.079 0.95
192_V 206_A 1.076 0.95
208_Q 329_D 1.076 0.95
28_G 73_E 1.076 0.95
336_D 339_P 1.075 0.95
37_T 140_T 1.074 0.95
69_V 80_P 1.071 0.95
222_K 225_D 1.064 0.95
188_D 258_D 1.058 0.95
33_K 143_E 1.056 0.95
215_I 234_D 1.055 0.95
355_D 358_H 1.037 0.94
20_I 59_V 1.028 0.94
324_P 328_E 1.019 0.93
174_G 205_G 1.017 0.93
206_A 211_A 1.01 0.93
141_F 350_I 1.005 0.93
184_V 188_D 1 0.92
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
1cdoA20.99731000.095Contact Map0.802
1e3iA20.98921000.097Contact Map0.83
3ukoA20.99731000.098Contact Map0.754
2jhfA20.99731000.101Contact Map0.799
1p0fA20.99191000.105Contact Map0.799
2fzwA211000.106Contact Map0.783
1f8fA10.98371000.127Contact Map0.845
1vj0A40.94581000.159Contact Map0.795
4ej6A10.91331000.161Contact Map0.778
1h2bA20.90791000.171Contact Map0.806
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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