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OPENSEQ.org
TDH - L-threonine 3-dehydrogenase
UniProt: P07913 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG10993
Length: 341 (340)
Sequences: 22772
Seq/Len: 66.98
TDH
Paralog alert: 0.83 [within 20: 0.37] - ratio of genomes with paralogs
Cluster includes: ACUI ADHP CURA FRMA GATD IDND QOR1 RSPB TDH YAHK YBDR YCJQ YDJJ YDJL YGGP YJGB YJJN YPHC
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
320_D 323_K 3.475 1.00
33_R 141_D 3.231 1.00
31_K 70_V 3.142 1.00
33_R 68_E 2.954 1.00
28_L 132_A 2.913 1.00
29_L 70_V 2.833 1.00
7_L 16_T 2.808 1.00
140_S 143_L 2.763 1.00
184_K 210_T 2.756 1.00
83_D 134_K 2.721 1.00
202_E 206_K 2.66 1.00
316_F 327_A 2.566 1.00
201_L 205_R 2.535 1.00
80_K 83_D 2.526 1.00
223_V 226_E 2.482 1.00
25_H 74_Q 2.458 1.00
142_D 315_R 2.429 1.00
26_N 75_E 2.416 1.00
31_K 68_E 2.409 1.00
84_R 135_I 2.373 1.00
166_D 190_N 2.364 1.00
31_K 124_Y 2.315 1.00
5_S 16_T 2.284 1.00
317_S 320_D 2.243 1.00
78_G 129_A 2.22 1.00
167_V 183_A 2.216 1.00
25_H 75_E 2.206 1.00
20_V 124_Y 2.188 1.00
300_A 304_S 2.151 1.00
7_L 14_W 2.084 1.00
198_E 202_E 2.075 1.00
22_E 71_G 2.058 1.00
190_N 211_R 2.052 1.00
84_R 141_D 2.051 1.00
315_R 339_S 2.045 1.00
168_L 233_F 2.037 1.00
180_A 209_I 2.021 1.00
165_E 258_R 2.019 1.00
65_Y 120_C 2.017 1.00
314_H 327_A 1.999 1.00
162_L 165_E 1.989 1.00
202_E 205_R 1.982 1.00
323_K 326_D 1.969 1.00
24_G 27_D 1.968 1.00
326_D 330_S 1.966 1.00
253_M 259_I 1.939 1.00
29_L 126_V 1.93 1.00
66_V 141_D 1.915 1.00
168_L 194_T 1.912 1.00
198_E 214_N 1.905 1.00
316_F 324_G 1.879 1.00
70_V 124_Y 1.873 1.00
327_A 332_Q 1.795 1.00
143_L 309_S 1.792 1.00
234_D 254_N 1.761 1.00
46_N 329_R 1.756 1.00
2_K 17_D 1.754 1.00
201_L 212_A 1.751 1.00
258_R 282_F 1.743 1.00
135_I 144_A 1.738 1.00
304_S 307_D 1.728 1.00
79_F 134_K 1.725 1.00
65_Y 87_G 1.724 1.00
195_D 201_L 1.723 1.00
84_R 137_D 1.712 1.00
71_G 81_I 1.71 1.00
72_I 81_I 1.679 1.00
253_M 257_G 1.678 1.00
323_K 327_A 1.672 1.00
218_E 223_V 1.672 1.00
73_G 76_V 1.661 1.00
203_L 206_K 1.657 1.00
214_N 217_K 1.646 1.00
219_N 222_D 1.644 1.00
253_M 281_L 1.631 1.00
4_L 321_F 1.627 1.00
27_D 128_P 1.625 1.00
258_R 284_K 1.62 1.00
135_I 141_D 1.611 1.00
23_L 126_V 1.604 1.00
192_V 233_F 1.604 1.00
138_N 303_Q 1.586 1.00
180_A 193_I 1.573 1.00
170_S 249_M 1.567 1.00
198_E 217_K 1.556 1.00
211_R 227_L 1.546 1.00
236_G 249_M 1.541 1.00
68_E 84_R 1.537 1.00
26_N 128_P 1.507 1.00
2_K 123_E 1.503 1.00
79_F 83_D 1.499 1.00
34_K 339_S 1.489 1.00
32_I 65_Y 1.486 1.00
150_F 178_M 1.482 1.00
29_L 124_Y 1.472 1.00
2_K 319_D 1.469 1.00
27_D 126_V 1.467 1.00
69_V 79_F 1.458 1.00
158_L 186_V 1.445 1.00
28_L 79_F 1.438 1.00
29_L 71_G 1.436 1.00
121_F 325_F 1.417 1.00
3_A 18_V 1.403 0.99
142_D 339_S 1.4 0.99
172_A 193_I 1.399 0.99
236_G 253_M 1.385 0.99
34_K 142_D 1.381 0.99
322_Q 326_D 1.374 0.99
96_C 99_C 1.362 0.99
23_L 27_D 1.36 0.99
166_D 192_V 1.357 0.99
68_E 141_D 1.354 0.99
179_A 237_L 1.35 0.99
313_T 327_A 1.345 0.99
23_L 29_L 1.335 0.99
190_N 210_T 1.329 0.99
8_K 11_E 1.328 0.99
4_L 15_M 1.326 0.99
196_V 215_V 1.313 0.99
79_F 132_A 1.312 0.99
222_D 226_E 1.307 0.99
185_H 307_D 1.287 0.99
154_V 158_L 1.272 0.99
33_R 84_R 1.269 0.99
30_I 67_G 1.253 0.99
170_S 194_T 1.242 0.98
93_C 96_C 1.232 0.98
127_I 132_A 1.217 0.98
193_I 209_I 1.204 0.98
37_I 324_G 1.197 0.98
45_Y 325_F 1.19 0.98
26_N 129_A 1.187 0.98
205_R 212_A 1.183 0.98
198_E 201_L 1.183 0.98
166_D 234_D 1.182 0.98
166_D 189_R 1.178 0.98
99_C 107_C 1.168 0.97
34_K 141_D 1.167 0.97
299_A 303_Q 1.154 0.97
20_V 29_L 1.149 0.97
300_A 303_Q 1.147 0.97
96_C 107_C 1.145 0.97
223_V 227_L 1.136 0.97
297_K 300_A 1.128 0.97
4_L 318_I 1.108 0.96
169_V 179_A 1.107 0.96
15_M 319_D 1.1 0.96
206_K 310_P 1.099 0.96
32_I 125_L 1.098 0.96
158_L 297_K 1.097 0.96
318_I 340_W 1.096 0.96
205_R 211_R 1.069 0.95
321_F 338_L 1.054 0.95
72_I 76_V 1.053 0.95
238_E 241_G 1.052 0.95
30_I 85_V 1.052 0.95
317_S 339_S 1.049 0.94
307_D 310_P 1.044 0.94
199_Y 202_E 1.041 0.94
149_P 152_N 1.036 0.94
36_A 64_E 1.034 0.94
326_D 329_R 1.034 0.94
165_E 234_D 1.032 0.94
299_A 304_S 1.028 0.94
38_C 63_H 1.026 0.94
201_L 214_N 1.022 0.93
33_R 66_V 1.02 0.93
196_V 216_A 1.016 0.93
93_C 99_C 1.013 0.93
5_S 18_V 1.012 0.93
295_W 299_A 1.01 0.93
192_V 211_R 1.01 0.93
296_Y 300_A 1.01 0.93
183_A 188_A 1.008 0.93
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
2dq4A20.99411000.053Contact Map0.815
4a2cA20.97651000.058Contact Map0.75
4ej6A10.97651000.06Contact Map0.77
2d8aA111000.061Contact Map0.822
1h2bA20.98241000.068Contact Map0.814
1e3iA20.97951000.073Contact Map0.825
4ilkA20.97361000.074Contact Map0.796
1f8fA10.97951000.079Contact Map0.84
4eezA20.97651000.079Contact Map0.818
1vj0A40.98531000.08Contact Map0.804
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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