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OPENSEQ.org
NIKB - Nickel transport system permease protein NikB
UniProt: P33591 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG12076
Length: 314 (305)
Sequences: 6383
Seq/Len: 20.93
NIKB
Paralog alert: 0.81 [within 20: 0.12] - ratio of genomes with paralogs
Cluster includes: DDPB DDPC DPPB DPPC GSIC GSID NIKB NIKC OPPB OPPC SAPB SAPC YEJB YEJE
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
62_Q 66_V 3.706 1.00
56_T 61_D 3.691 1.00
28_R 67_Q 3.486 1.00
62_Q 70_T 3.425 1.00
73_W 77_H 3.257 1.00
20_S 68_Y 3.256 1.00
33_D 55_R 3.134 1.00
48_P 52_A 3.127 1.00
69_G 73_W 2.955 1.00
21_V 68_Y 2.782 1.00
212_R 218_D 2.72 1.00
100_A 258_W 2.659 1.00
199_M 226_I 2.627 1.00
208_V 226_I 2.602 1.00
220_Q 224_R 2.591 1.00
107_A 180_A 2.486 1.00
235_I 302_L 2.461 1.00
65_Y 69_G 2.453 1.00
165_L 177_I 2.249 1.00
93_D 264_Y 2.179 1.00
263_R 267_S 2.15 1.00
111_L 184_A 2.103 1.00
239_G 295_V 2.088 1.00
19_A 285_V 2.036 1.00
210_W 214_R 2.026 1.00
203_A 226_I 1.959 1.00
236_T 299_N 1.916 1.00
74_K 79_D 1.905 1.00
130_L 134_A 1.881 1.00
3_R 7_R 1.806 1.00
89_P 92_D 1.798 1.00
16_V 286_V 1.789 1.00
75_A 80_F 1.789 1.00
53_S 57_M 1.78 1.00
51_L 55_R 1.779 1.00
52_A 56_T 1.776 1.00
66_V 70_T 1.775 1.00
50_M 53_S 1.763 1.00
217_N 220_Q 1.749 1.00
202_V 207_H 1.746 1.00
20_S 71_W 1.744 1.00
86_S 93_D 1.728 1.00
126_H 129_R 1.727 1.00
114_L 118_P 1.678 1.00
17_L 21_V 1.672 1.00
88_R 92_D 1.668 1.00
298_L 301_A 1.666 1.00
154_L 182_S 1.654 1.00
159_S 167_A 1.634 1.00
165_L 174_Q 1.63 1.00
10_L 14_P 1.607 1.00
91_L 95_L 1.599 1.00
47_T 50_M 1.592 1.00
125_R 222_E 1.574 1.00
52_A 55_R 1.57 1.00
12_L 289_V 1.563 1.00
119_L 135_V 1.562 1.00
115_T 118_P 1.56 1.00
90_V 279_C 1.546 1.00
268_A 276_V 1.536 1.00
156_M 161_Y 1.514 1.00
202_V 205_Q 1.513 1.00
88_R 96_N 1.509 1.00
184_A 188_L 1.509 1.00
122_W 126_H 1.504 1.00
149_W 153_L 1.495 1.00
124_A 200_L 1.484 1.00
132_D 196_R 1.483 1.00
59_G 62_Q 1.478 1.00
157_A 161_Y 1.466 1.00
202_V 208_V 1.465 1.00
70_T 73_W 1.462 1.00
17_L 72_L 1.458 1.00
219_K 223_R 1.454 1.00
35_A 51_L 1.453 1.00
207_H 229_N 1.451 1.00
214_R 225_H 1.449 1.00
66_V 69_G 1.44 1.00
128_D 132_D 1.439 1.00
17_L 68_Y 1.438 1.00
153_L 157_A 1.421 1.00
206_R 209_T 1.413 1.00
165_L 178_L 1.408 1.00
142_G 189_A 1.401 0.99
264_Y 276_V 1.39 0.99
131_P 135_V 1.373 0.99
16_V 289_V 1.37 0.99
100_A 103_E 1.368 0.99
202_V 230_A 1.352 0.99
265_A 280_F 1.35 0.99
115_T 188_L 1.35 0.99
93_D 276_V 1.349 0.99
63_P 66_V 1.344 0.99
208_V 221_T 1.338 0.99
122_W 131_P 1.335 0.99
21_V 64_L 1.32 0.99
80_F 279_C 1.318 0.99
120_G 195_L 1.307 0.99
59_G 70_T 1.306 0.99
113_L 238_V 1.303 0.99
32_G 272_R 1.301 0.99
145_M 150_L 1.296 0.99
12_L 293_L 1.285 0.99
232_L 299_N 1.278 0.99
157_A 162_L 1.276 0.99
180_A 184_A 1.276 0.99
112_I 187_S 1.274 0.99
150_L 185_F 1.253 0.99
6_L 10_L 1.244 0.98
126_H 131_P 1.244 0.98
280_F 284_M 1.243 0.98
135_V 192_A 1.234 0.98
54_T 58_L 1.234 0.98
121_I 199_M 1.223 0.98
154_L 178_L 1.218 0.98
70_T 74_K 1.213 0.98
111_L 180_A 1.206 0.98
28_R 64_L 1.198 0.98
158_F 162_L 1.186 0.98
116_S 191_N 1.183 0.98
294_I 298_L 1.183 0.98
20_S 282_L 1.182 0.98
21_V 25_L 1.181 0.98
124_A 199_M 1.18 0.98
147_N 252_I 1.158 0.97
297_L 301_A 1.148 0.97
92_D 95_L 1.147 0.97
83_S 273_D 1.124 0.97
103_E 176_I 1.124 0.97
151_A 182_S 1.119 0.96
128_D 133_F 1.118 0.96
106_G 110_V 1.104 0.96
120_G 124_A 1.097 0.96
109_L 238_V 1.094 0.96
96_N 99_P 1.089 0.96
177_I 181_V 1.087 0.96
68_Y 72_L 1.079 0.95
151_A 255_I 1.075 0.95
249_T 252_I 1.073 0.95
5_V 297_L 1.072 0.95
4_Y 8_R 1.072 0.95
116_S 195_L 1.054 0.95
88_R 93_D 1.051 0.94
195_L 234_M 1.04 0.94
62_Q 65_Y 1.037 0.94
56_T 62_Q 1.036 0.94
80_F 91_L 1.033 0.94
54_T 57_M 1.032 0.94
194_L 237_A 1.03 0.94
291_C 295_V 1.02 0.93
170_Y 258_W 1.016 0.93
103_E 170_Y 1.011 0.93
216_L 220_Q 1.011 0.93
75_A 282_L 1.01 0.93
158_F 164_W 1.008 0.93
25_L 64_L 1.007 0.93
39_L 46_P 1.004 0.93
124_A 203_A 1.003 0.93
218_D 221_T 1.002 0.92
39_L 54_T 1.001 0.92
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
3tuiA40.668899.90.741Contact Map0.728
3rlfF10.910899.80.763Contact Map0.54
2onkC40.837699.80.765Contact Map0.572
3d31C20.840899.80.779Contact Map0.544
3rlfG10.904599.60.809Contact Map0.407
3s82A20.277120.10.965Contact Map0.275
1p7lA40.286612.90.968Contact Map0.378
2p02A10.26759.50.97Contact Map0.278
2yevC20.10838.90.97Contact Map0.646
3aqpA20.84716.70.972Contact Map0.386
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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