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OPENSEQ.org
PUUP - Putrescine importer PuuP
UniProt: P76037 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG13907
Length: 461 (433)
Sequences: 10718
Seq/Len: 24.75
PUUP
Paralog alert: 0.82 [within 20: 0.37] - ratio of genomes with paralogs
Cluster includes: ADIC ANSP ARCD AROP CADB CYCA FRLA GABP GADC LYSP MMUP PHEP PLAP POTE PROY PUUP YBAT YCAM YGJI YIFK YJEH YJEM
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
329_V 342_L 4.132 1.00
146_T 348_G 3.503 1.00
375_T 409_A 3.174 1.00
23_K 232_P 3.1 1.00
320_G 331_G 3.099 1.00
178_V 259_F 3.029 1.00
332_Y 342_L 3.013 1.00
63_L 250_G 3.012 1.00
236_R 240_K 2.978 1.00
233_D 236_R 2.923 1.00
139_V 355_L 2.893 1.00
378_N 408_G 2.636 1.00
345_I 349_I 2.494 1.00
288_L 292_I 2.491 1.00
334_H 337_W 2.469 1.00
383_N 387_R 2.451 1.00
406_L 410_L 2.358 1.00
231_T 237_V 2.349 1.00
334_H 341_A 2.349 1.00
329_V 332_Y 2.324 1.00
28_V 244_L 2.292 1.00
323_N 327_E 2.27 1.00
103_G 319_M 2.267 1.00
317_Y 321_R 2.245 1.00
24_L 244_L 2.218 1.00
176_F 180_Q 2.197 1.00
24_L 237_V 2.174 1.00
87_Y 322_D 2.165 1.00
54_H 207_I 2.153 1.00
72_S 378_N 2.095 1.00
91_Q 96_P 2.054 1.00
330_F 346_M 2.027 1.00
229_E 321_R 2.002 1.00
91_Q 99_G 1.998 1.00
18_L 333_V 1.983 1.00
23_K 26_Q 1.963 1.00
64_A 407_V 1.898 1.00
24_L 28_V 1.886 1.00
147_A 151_K 1.885 1.00
64_A 68_F 1.88 1.00
94_I 383_N 1.875 1.00
146_T 344_V 1.867 1.00
107_L 347_V 1.863 1.00
230_E 314_R 1.853 1.00
240_K 244_L 1.839 1.00
269_R 283_Y 1.835 1.00
27_V 231_T 1.823 1.00
168_I 172_V 1.822 1.00
94_I 379_L 1.81 1.00
150_L 341_A 1.803 1.00
385_F 389_K 1.797 1.00
379_L 409_A 1.768 1.00
403_L 407_V 1.75 1.00
154_N 158_N 1.733 1.00
383_N 386_W 1.731 1.00
150_L 345_I 1.717 1.00
410_L 414_V 1.714 1.00
233_D 237_V 1.706 1.00
247_V 251_V 1.701 1.00
47_V 55_V 1.694 1.00
28_V 248_Y 1.681 1.00
58_S 210_G 1.673 1.00
65_G 411_T 1.659 1.00
90_A 102_V 1.648 1.00
165_L 169_S 1.639 1.00
243_F 247_V 1.634 1.00
428_L 432_S 1.611 1.00
26_Q 161_T 1.603 1.00
60_L 254_I 1.601 1.00
279_E 282_L 1.582 1.00
22_L 26_Q 1.575 1.00
102_V 376_F 1.571 1.00
234_A 239_P 1.546 1.00
233_D 240_K 1.542 1.00
279_E 283_Y 1.521 1.00
400_H 404_M 1.516 1.00
313_S 344_V 1.498 1.00
312_V 347_V 1.497 1.00
316_L 343_N 1.491 1.00
104_W 434_G 1.475 1.00
409_A 413_G 1.472 1.00
90_A 376_F 1.466 1.00
342_L 346_M 1.435 1.00
80_F 92_K 1.43 1.00
327_E 331_G 1.428 1.00
291_S 295_C 1.422 1.00
95_N 98_V 1.41 1.00
175_I 255_A 1.405 0.99
48_S 55_V 1.4 0.99
62_A 214_V 1.397 0.99
125_L 137_W 1.393 0.99
143_A 348_G 1.392 0.99
24_L 240_K 1.392 0.99
72_S 405_P 1.39 0.99
142_V 351_A 1.386 0.99
227_L 310_A 1.382 0.99
32_L 248_Y 1.38 0.99
70_A 246_A 1.379 0.99
94_I 98_V 1.377 0.99
35_L 249_G 1.373 0.99
68_F 407_V 1.364 0.99
265_P 269_R 1.364 0.99
22_L 157_A 1.361 0.99
337_W 341_A 1.361 0.99
169_S 173_V 1.358 0.99
385_F 388_R 1.355 0.99
286_G 289_F 1.354 0.99
75_K 385_F 1.354 0.99
151_K 337_W 1.35 0.99
282_L 290_Q 1.322 0.99
93_S 380_S 1.318 0.99
178_V 263_F 1.318 0.99
425_T 429_V 1.317 0.99
149_N 306_L 1.309 0.99
56_P 258_F 1.303 0.99
285_G 289_F 1.297 0.99
237_V 240_K 1.296 0.99
118_V 142_V 1.295 0.99
145_L 305_G 1.294 0.99
100_F 104_W 1.292 0.99
282_L 287_K 1.278 0.99
78_R 394_S 1.268 0.99
327_E 332_Y 1.266 0.99
121_A 298_F 1.262 0.99
146_T 345_I 1.259 0.99
388_R 391_M 1.251 0.99
142_V 348_G 1.25 0.99
375_T 379_L 1.248 0.99
70_A 242_I 1.242 0.98
382_F 406_L 1.242 0.98
334_H 339_T 1.241 0.98
118_V 305_G 1.24 0.98
77_V 89_Y 1.239 0.98
31_G 245_T 1.238 0.98
83_A 229_E 1.235 0.98
333_V 338_R 1.235 0.98
332_Y 341_A 1.231 0.98
69_T 378_N 1.218 0.98
316_L 325_F 1.201 0.98
146_T 150_L 1.2 0.98
153_V 310_A 1.199 0.98
57_A 207_I 1.196 0.98
139_V 143_A 1.193 0.98
321_R 331_G 1.192 0.98
22_L 27_V 1.184 0.98
342_L 345_I 1.177 0.98
25_W 158_N 1.167 0.97
87_Y 318_V 1.165 0.97
386_W 389_K 1.165 0.97
19_R 338_R 1.161 0.97
104_W 325_F 1.159 0.97
42_D 120_L 1.154 0.97
114_P 351_A 1.151 0.97
381_V 405_P 1.148 0.97
147_A 150_L 1.146 0.97
432_S 436_A 1.143 0.97
219_L 370_A 1.141 0.97
60_L 64_A 1.141 0.97
429_V 433_L 1.14 0.97
82_E 88_T 1.138 0.97
90_A 98_V 1.138 0.97
402_L 406_L 1.137 0.97
265_P 268_S 1.133 0.97
251_V 255_A 1.127 0.97
88_T 92_K 1.127 0.97
410_L 413_G 1.126 0.97
170_I 296_T 1.119 0.96
317_Y 333_V 1.117 0.96
198_I 207_I 1.116 0.96
273_P 279_E 1.114 0.96
168_I 252_I 1.112 0.96
326_P 329_V 1.11 0.96
25_W 29_M 1.11 0.96
255_A 259_F 1.108 0.96
179_V 259_F 1.108 0.96
386_W 390_G 1.107 0.96
379_L 383_N 1.107 0.96
26_Q 158_N 1.106 0.96
204_L 207_I 1.105 0.96
436_A 440_Y 1.105 0.96
63_L 218_F 1.104 0.96
21_S 154_N 1.098 0.96
426_L 429_V 1.097 0.96
384_H 387_R 1.096 0.96
389_K 393_K 1.094 0.96
150_L 339_T 1.086 0.96
375_T 413_G 1.086 0.96
150_L 334_H 1.082 0.96
232_P 237_V 1.082 0.96
27_V 241_A 1.073 0.95
68_F 404_M 1.071 0.95
18_L 317_Y 1.069 0.95
421_S 425_T 1.067 0.95
47_V 51_S 1.067 0.95
159_F 163_F 1.061 0.95
384_H 388_R 1.06 0.95
222_D 226_T 1.058 0.95
37_P 253_F 1.054 0.95
31_G 224_V 1.052 0.95
71_I 243_F 1.048 0.94
116_I 216_F 1.044 0.94
84_G 87_Y 1.043 0.94
73_Y 86_A 1.038 0.94
90_A 380_S 1.037 0.94
163_F 303_A 1.034 0.94
21_S 232_P 1.031 0.94
250_G 254_I 1.028 0.94
180_Q 185_G 1.023 0.93
57_A 60_L 1.018 0.93
121_A 141_F 1.016 0.93
265_P 283_Y 1.015 0.93
174_F 178_V 1.011 0.93
26_Q 157_A 1.01 0.93
135_W 139_V 1.007 0.93
172_V 176_F 1.007 0.93
173_V 177_L 1.004 0.93
228_S 238_I 1.003 0.93
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
4djkA20.9611000.376Contact Map0.595
3l1lA10.93711000.381Contact Map0.682
3giaA10.92191000.403Contact Map0.622
3dh4A40.9479980.901Contact Map0.454
2jlnA10.9197980.903Contact Map0.427
2xq2A10.954497.40.913Contact Map0.543
4m48A10.928494.20.935Contact Map0.371
2a65A10.911191.20.941Contact Map0.388
2kluA10.112820.60.967Contact Map0.25
1m56C20.15629.80.971Contact Map0.589
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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