May 4, 2021 - We are working on upgrading the webserver, some pages may not work.
OPENSEQ.org
YJEM - Inner membrane transporter YjeM
UniProt: P39282 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG12475
Length: 500 (475)
Sequences: 7514
Seq/Len: 15.82
YJEM
Paralog alert: 0.83 [within 20: 0.28] - ratio of genomes with paralogs
Cluster includes: ADIC ANSP ARCD AROP CADB CYCA FRLA GABP GADC LYSP MMUP PHEP PLAP POTE PROY PUUP YBAT YCAM YGJI YIFK YJEH YJEM
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
351_P 363_I 4.31 1.00
343_T 353_T 3.971 1.00
9_S 234_E 3.511 1.00
178_S 261_L 3.342 1.00
311_W 315_I 3.337 1.00
235_N 238_K 3.236 1.00
146_T 369_C 3.158 1.00
346_A 350_E 2.817 1.00
238_K 242_K 2.775 1.00
366_W 370_G 2.738 1.00
414_F 417_A 2.723 1.00
406_L 442_V 2.711 1.00
352_M 367_M 2.689 1.00
233_T 239_N 2.556 1.00
354_R 363_I 2.548 1.00
46_A 252_S 2.474 1.00
267_N 270_Q 2.461 1.00
356_N 362_S 2.423 1.00
9_S 12_G 2.285 1.00
340_I 344_P 2.279 1.00
231_D 344_P 2.269 1.00
76_N 84_A 2.25 1.00
351_P 354_R 2.223 1.00
14_I 246_F 2.172 1.00
92_F 368_Q 2.149 1.00
88_T 342_G 2.089 1.00
56_M 409_A 2.086 1.00
79_V 414_F 2.07 1.00
137_L 377_L 2.049 1.00
363_I 367_M 1.974 1.00
72_Y 345_K 1.919 1.00
270_Q 289_S 1.896 1.00
412_F 434_A 1.891 1.00
146_T 365_M 1.887 1.00
237_E 424_P 1.879 1.00
429_K 433_S 1.877 1.00
13_L 233_T 1.873 1.00
56_M 434_A 1.867 1.00
79_V 410_L 1.847 1.00
435_M 439_V 1.841 1.00
10_L 14_I 1.827 1.00
89_F 348_W 1.805 1.00
43_I 256_S 1.796 1.00
59_E 416_K 1.743 1.00
314_R 318_L 1.739 1.00
76_N 81_P 1.732 1.00
8_M 157_A 1.731 1.00
165_I 169_C 1.73 1.00
336_L 365_M 1.727 1.00
41_F 212_F 1.724 1.00
8_M 12_G 1.71 1.00
413_P 435_M 1.701 1.00
10_L 246_F 1.701 1.00
61_G 74_W 1.698 1.00
232_K 337_K 1.695 1.00
11_I 158_R 1.68 1.00
87_G 407_F 1.675 1.00
75_M 87_G 1.646 1.00
150_S 362_S 1.627 1.00
79_V 83_F 1.623 1.00
64_Y 77_N 1.621 1.00
150_S 366_W 1.619 1.00
350_E 353_T 1.608 1.00
10_L 239_N 1.598 1.00
185_N 188_H 1.591 1.00
432_L 436_I 1.577 1.00
147_V 151_K 1.575 1.00
176_L 180_T 1.574 1.00
339_I 348_W 1.565 1.00
221_G 401_M 1.559 1.00
67_E 231_D 1.557 1.00
442_V 446_T 1.544 1.00
236_P 241_A 1.542 1.00
12_G 161_A 1.539 1.00
62_A 424_P 1.519 1.00
145_V 328_F 1.515 1.00
4_T 355_L 1.512 1.00
235_N 242_K 1.512 1.00
169_C 173_V 1.503 1.00
37_S 209_M 1.502 1.00
175_L 257_L 1.496 1.00
109_P 112_T 1.495 1.00
242_K 246_F 1.483 1.00
249_I 253_I 1.449 1.00
363_I 366_W 1.446 1.00
5_I 359_G 1.436 1.00
350_E 354_R 1.43 1.00
344_P 353_T 1.418 1.00
413_P 438_T 1.414 1.00
9_S 154_N 1.404 0.99
288_K 292_M 1.403 0.99
37_S 200_N 1.401 0.99
78_S 411_A 1.396 0.99
154_N 158_R 1.391 0.99
253_I 257_L 1.387 0.99
332_C 336_L 1.385 0.99
72_Y 88_T 1.379 0.99
107_W 110_F 1.378 0.99
409_A 441_V 1.378 0.99
406_L 410_L 1.376 0.99
146_T 366_W 1.371 0.99
370_G 374_V 1.366 0.99
14_I 17_I 1.366 0.99
46_A 436_I 1.364 0.99
75_M 411_A 1.36 0.99
358_M 362_S 1.359 0.99
133_V 137_L 1.348 0.99
356_N 360_M 1.346 0.99
224_E 228_G 1.342 0.99
443_L 447_F 1.337 0.99
189_F 192_D 1.336 0.99
197_A 200_N 1.334 0.99
339_I 364_A 1.327 0.99
47_L 51_I 1.323 0.99
72_Y 341_Q 1.31 0.99
235_N 239_N 1.308 0.99
335_P 368_Q 1.295 0.99
19_T 164_G 1.295 0.99
104_A 379_V 1.288 0.99
134_V 138_A 1.287 0.99
179_I 183_L 1.287 0.99
8_M 13_L 1.281 0.99
95_Y 331_L 1.279 0.99
39_I 256_S 1.274 0.99
469_M 472_G 1.273 0.99
450_V 454_I 1.272 0.99
14_I 250_V 1.27 0.99
92_F 96_I 1.263 0.99
85_F 89_F 1.263 0.99
150_S 356_N 1.26 0.99
12_G 158_R 1.253 0.99
158_R 161_A 1.246 0.98
419_Q 423_R 1.245 0.98
439_V 443_L 1.245 0.98
39_I 260_F 1.242 0.98
413_P 434_A 1.241 0.98
355_L 359_G 1.238 0.98
159_I 163_G 1.233 0.98
149_A 329_F 1.231 0.98
436_I 439_V 1.228 0.98
66_K 73_S 1.22 0.98
27_S 259_I 1.217 0.98
396_M 400_S 1.21 0.98
234_E 239_N 1.204 0.98
245_V 249_I 1.201 0.98
22_F 167_V 1.2 0.98
63_A 424_P 1.199 0.98
210_L 447_F 1.191 0.98
430_T 433_S 1.19 0.98
10_L 242_K 1.184 0.98
54_A 248_A 1.18 0.98
239_N 242_K 1.179 0.98
168_M 172_L 1.178 0.98
146_T 150_S 1.175 0.98
92_F 339_I 1.173 0.98
173_V 177_V 1.173 0.98
94_S 401_M 1.172 0.98
105_K 108_V 1.17 0.97
429_K 432_L 1.166 0.97
437_A 441_V 1.164 0.97
110_F 113_F 1.164 0.97
73_S 77_N 1.158 0.97
148_V 159_I 1.158 0.97
30_A 38_A 1.155 0.97
250_V 254_G 1.154 0.97
225_A 334_S 1.149 0.97
343_T 352_M 1.148 0.97
376_I 380_S 1.146 0.97
90_M 404_P 1.143 0.97
257_L 261_L 1.142 0.97
8_M 153_I 1.142 0.97
340_I 355_L 1.141 0.97
136_L 376_I 1.134 0.97
45_S 216_A 1.13 0.97
197_A 201_P 1.127 0.97
96_I 368_Q 1.117 0.96
153_I 333_Y 1.117 0.96
80_G 83_F 1.116 0.96
196_L 199_P 1.116 0.96
107_W 111_S 1.116 0.96
23_G 255_Y 1.112 0.96
69_G 72_Y 1.109 0.96
332_C 369_C 1.109 0.96
7_K 234_E 1.105 0.96
110_F 321_F 1.102 0.96
281_N 288_K 1.093 0.96
34_M 212_F 1.091 0.96
448_A 452_T 1.089 0.96
18_F 250_V 1.089 0.96
46_A 51_I 1.088 0.96
131_T 134_V 1.086 0.96
272_L 275_G 1.083 0.96
108_V 111_S 1.082 0.96
21_V 251_I 1.082 0.96
188_H 192_D 1.081 0.95
100_V 103_S 1.078 0.95
195_F 264_V 1.077 0.95
449_N 453_I 1.075 0.95
179_I 182_L 1.072 0.95
101_S 394_T 1.07 0.95
83_F 407_F 1.07 0.95
43_I 47_L 1.068 0.95
7_K 154_N 1.065 0.95
98_W 101_S 1.064 0.95
354_R 362_S 1.063 0.95
348_W 352_M 1.059 0.95
16_M 229_L 1.055 0.95
102_T 105_K 1.048 0.94
19_T 22_F 1.048 0.94
208_A 211_S 1.047 0.94
170_L 319_S 1.046 0.94
30_A 34_M 1.045 0.94
46_A 50_F 1.045 0.94
377_L 380_S 1.043 0.94
468_W 471_G 1.039 0.94
158_R 162_V 1.038 0.94
182_L 264_V 1.038 0.94
418_R 421_L 1.037 0.94
27_S 30_A 1.036 0.94
33_L 211_S 1.036 0.94
221_G 397_A 1.035 0.94
138_A 141_W 1.034 0.94
55_L 245_V 1.027 0.94
150_S 365_M 1.019 0.93
42_Y 46_A 1.019 0.93
410_L 414_F 1.018 0.93
151_K 358_M 1.016 0.93
374_V 377_L 1.014 0.93
76_N 345_K 1.014 0.93
117_S 120_T 1.006 0.93
339_I 367_M 1.005 0.93
423_R 426_V 1.004 0.93
311_W 314_R 1.003 0.93
99_M 372_V 1.002 0.92
177_V 308_L 1 0.92
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
4djkA20.931000.328Contact Map0.576
3giaA10.8621000.43Contact Map0.552
3l1lA10.8761000.443Contact Map0.673
2jlnA10.83897.80.906Contact Map0.483
2a65A10.9197.40.912Contact Map0.464
4m48A10.91296.40.924Contact Map0.428
3dh4A40.86895.70.928Contact Map0.375
2xq2A10.86694.20.934Contact Map0.461
3j1zP20.27832.60.963Contact Map0.272
2kluA10.08614.30.969Contact Map0.239
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
Page generated in 1.2763 seconds.