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OPENSEQ.org
MACA - Macrolide-specific efflux protein MacA
UniProt: P75830 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG13694
Length: 371 (334)
Sequences: 9991
Seq/Len: 29.91
MACA
Paralog alert: 0.76 [within 20: 0.15] - ratio of genomes with paralogs
Cluster includes: AAEA ACRA ACRE EMRA EMRK MACA MDTA MDTE MDTN YBHG YDHJ YHII YIAV YIBH
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
339_T 352_K 3.326 1.00
74_T 91_V 3.259 1.00
88_L 185_R 3.137 1.00
84_K 191_A 3.062 1.00
126_R 130_S 3.052 1.00
336_R 358_D 2.95 1.00
40_I 359_E 2.685 1.00
133_Q 137_Q 2.559 1.00
43_P 356_A 2.461 1.00
133_Q 150_A 2.412 1.00
57_D 226_K 2.367 1.00
132_Q 141_V 2.359 1.00
311_L 348_V 2.315 1.00
84_K 87_Q 2.298 1.00
257_G 279_F 2.275 1.00
56_L 225_V 2.24 1.00
67_Q 183_Y 2.228 1.00
91_V 185_R 2.222 1.00
136_A 146_L 2.16 1.00
76_S 91_V 2.156 1.00
143_Q 147_D 2.122 1.00
308_T 347_D 2.082 1.00
132_Q 149_A 2.081 1.00
76_S 88_L 2.073 1.00
88_L 187_V 2.073 1.00
44_G 347_D 2.042 1.00
242_K 256_E 2.03 1.00
227_A 296_V 2.017 1.00
260_K 280_E 2.014 1.00
122_L 153_M 2 1.00
150_A 154_A 1.997 1.00
44_G 308_T 1.987 1.00
355_E 358_D 1.966 1.00
195_T 218_A 1.945 1.00
43_P 305_N 1.938 1.00
341_G 351_V 1.919 1.00
82_K 193_E 1.916 1.00
188_A 217_L 1.914 1.00
41_V 357_G 1.906 1.00
72_L 199_T 1.861 1.00
341_G 349_E 1.854 1.00
124_L 128_T 1.848 1.00
168_K 171_Q 1.847 1.00
129_Y 146_L 1.829 1.00
175_D 178_K 1.818 1.00
85_K 191_A 1.803 1.00
339_T 351_V 1.794 1.00
333_T 361_V 1.779 1.00
136_A 143_Q 1.75 1.00
338_V 350_I 1.739 1.00
327_L 336_R 1.738 1.00
101_I 174_L 1.725 1.00
116_Q 119_E 1.723 1.00
325_V 328_L 1.723 1.00
244_W 256_E 1.717 1.00
242_K 258_Q 1.717 1.00
340_I 348_V 1.715 1.00
119_E 123_K 1.704 1.00
324_K 335_E 1.678 1.00
108_L 174_L 1.673 1.00
244_W 254_R 1.671 1.00
126_R 153_M 1.67 1.00
129_Y 133_Q 1.659 1.00
227_A 279_F 1.643 1.00
253_T 287_L 1.626 1.00
171_Q 175_D 1.622 1.00
49_S 297_H 1.604 1.00
342_A 349_E 1.584 1.00
70_G 92_I 1.58 1.00
94_P 98_E 1.575 1.00
56_L 223_M 1.57 1.00
72_L 90_G 1.566 1.00
122_L 126_R 1.548 1.00
324_K 328_L 1.542 1.00
145_D 148_T 1.541 1.00
45_D 304_K 1.534 1.00
129_Y 153_M 1.525 1.00
303_V 349_E 1.525 1.00
41_V 307_L 1.516 1.00
243_A 279_F 1.503 1.00
230_S 233_D 1.49 1.00
126_R 157_Q 1.479 1.00
194_V 215_L 1.476 1.00
329_R 334_R 1.474 1.00
219_D 222_A 1.471 1.00
60_R 219_D 1.467 1.00
130_S 133_Q 1.46 1.00
123_K 126_R 1.454 1.00
309_I 360_V 1.444 1.00
78_A 81_D 1.438 1.00
51_L 295_Q 1.415 1.00
161_G 164_D 1.412 1.00
225_V 296_V 1.387 0.99
247_V 294_A 1.38 0.99
307_L 356_A 1.373 0.99
224_L 278_R 1.369 0.99
109_M 112_R 1.365 0.99
74_T 201_Q 1.354 0.99
148_T 152_E 1.354 0.99
119_E 171_Q 1.351 0.99
311_L 346_T 1.35 0.99
51_L 297_H 1.343 0.99
226_K 276_Y 1.342 0.99
89_L 215_L 1.338 0.99
225_V 294_A 1.338 0.99
115_R 119_E 1.331 0.99
327_L 358_D 1.324 0.99
105_E 178_K 1.306 0.99
244_W 297_H 1.304 0.99
61_K 216_T 1.285 0.99
38_T 359_E 1.28 0.99
146_L 150_A 1.267 0.99
45_D 305_N 1.266 0.99
112_R 178_K 1.254 0.99
63_D 213_N 1.252 0.99
317_P 335_E 1.251 0.99
39_L 362_I 1.245 0.98
97_A 184_T 1.241 0.98
105_E 109_M 1.236 0.98
90_G 215_L 1.23 0.98
267_E 270_N 1.226 0.98
228_Q 276_Y 1.225 0.98
174_L 178_K 1.219 0.98
123_K 127_V 1.204 0.98
129_Y 150_A 1.194 0.98
98_E 181_L 1.19 0.98
197_I 200_L 1.183 0.98
306_V 349_E 1.178 0.98
178_K 182_D 1.171 0.98
75_L 201_Q 1.17 0.97
350_I 354_L 1.17 0.97
258_Q 280_E 1.17 0.97
144_Q 148_T 1.167 0.97
73_K 93_D 1.166 0.97
77_V 87_Q 1.159 0.97
117_Q 121_E 1.145 0.97
188_A 191_A 1.142 0.97
246_T 297_H 1.14 0.97
115_R 167_I 1.138 0.97
245_F 281_V 1.132 0.97
88_L 91_V 1.13 0.97
82_K 286_G 1.129 0.97
131_R 147_D 1.128 0.97
128_T 132_Q 1.127 0.97
314_L 348_V 1.123 0.97
81_D 87_Q 1.121 0.97
40_I 357_G 1.116 0.96
134_R 138_T 1.114 0.96
244_W 299_Q 1.114 0.96
114_Q 117_Q 1.114 0.96
266_P 275_Y 1.112 0.96
176_T 180_N 1.106 0.96
123_K 154_A 1.104 0.96
133_Q 146_L 1.104 0.96
37_Q 363_G 1.092 0.96
63_D 216_T 1.09 0.96
225_V 245_F 1.088 0.96
128_T 148_T 1.082 0.96
190_M 217_L 1.079 0.95
79_I 196_Q 1.071 0.95
197_I 215_L 1.055 0.95
164_D 168_K 1.055 0.95
196_Q 216_T 1.048 0.94
311_L 343_R 1.047 0.94
130_S 134_R 1.046 0.94
322_R 337_E 1.04 0.94
115_R 174_L 1.035 0.94
344_N 347_D 1.03 0.94
77_V 215_L 1.027 0.94
66_A 186_I 1.023 0.93
62_V 190_M 1.016 0.93
90_G 186_I 1.011 0.93
246_T 254_R 1.006 0.93
73_K 94_P 1.005 0.93
139_K 143_Q 1.004 0.93
222_A 282_P 1.002 0.92
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
3fppA20.91911000.215Contact Map0.77
4dk0A10.91641000.236Contact Map0.625
3lnnA20.91641000.319Contact Map0.759
1vf7A130.86251000.321Contact Map0.838
4l8jA10.83291000.337Contact Map0.622
3ne5B20.87331000.363Contact Map0.776
4kkuA40.71431000.423Contact Map0.771
2f1mA40.70891000.43Contact Map0.768
2k32A10.3199.90.716Contact Map0.633
2dneA10.274999.60.78Contact Map0.63
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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