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OPENSEQ.org
POTB - Spermidine/putrescine transport system permease protein PotB
UniProt: P0AFK4 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG10750
Length: 275 (266)
Sequences: 23993
Seq/Len: 90.20
POTB
Paralog alert: 0.95 [within 20: 0.85] - ratio of genomes with paralogs
Cluster includes: CYST CYSW MALG MODB POTB POTC POTH POTI PSTA PSTC UGPA UGPE YCJO YCJP YDCU YDCV
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
183_Q 187_R 4.411 1.00
24_S 43_Y 3.657 1.00
56_L 60_N 3.285 1.00
180_S 183_Q 2.784 1.00
182_L 186_I 2.746 1.00
171_L 189_I 2.622 1.00
175_R 184_T 2.6 1.00
41_D 44_T 2.523 1.00
160_Y 164_E 2.517 1.00
41_D 45_R 2.474 1.00
54_V 229_V 2.458 1.00
52_F 56_L 2.356 1.00
68_A 148_V 2.336 1.00
159_L 197_I 2.325 1.00
53_E 56_L 2.289 1.00
181_K 184_T 2.269 1.00
82_K 171_L 2.251 1.00
163_I 193_T 2.242 1.00
174_A 184_T 2.232 1.00
74_Y 185_F 2.207 1.00
64_I 144_I 2.129 1.00
66_T 201_C 2.101 1.00
179_A 183_Q 2.062 1.00
224_L 232_V 2.028 1.00
155_M 200_G 2.015 1.00
183_Q 186_I 1.952 1.00
81_A 164_E 1.921 1.00
162_S 196_G 1.919 1.00
62_A 205_M 1.85 1.00
21_I 248_I 1.848 1.00
175_R 181_K 1.838 1.00
85_H 88_R 1.825 1.00
74_Y 193_T 1.812 1.00
239_D 242_F 1.801 1.00
170_L 192_L 1.75 1.00
43_Y 248_I 1.74 1.00
174_A 179_A 1.732 1.00
73_G 155_M 1.723 1.00
70_L 201_C 1.717 1.00
18_L 22_G 1.706 1.00
53_E 57_H 1.684 1.00
171_L 184_T 1.678 1.00
124_L 129_V 1.659 1.00
74_Y 159_L 1.626 1.00
84_P 88_R 1.62 1.00
22_G 26_L 1.613 1.00
60_N 64_I 1.579 1.00
212_F 231_K 1.573 1.00
190_I 197_I 1.556 1.00
45_R 242_F 1.539 1.00
171_L 175_R 1.481 1.00
39_T 42_N 1.48 1.00
40_L 44_T 1.468 1.00
60_N 140_P 1.452 1.00
82_K 168_K 1.452 1.00
76_F 156_V 1.451 1.00
180_S 184_T 1.442 1.00
81_A 160_Y 1.436 1.00
77_A 159_L 1.427 1.00
121_N 136_I 1.421 1.00
212_F 216_D 1.42 1.00
71_V 75_P 1.406 0.99
155_M 204_V 1.398 0.99
80_L 156_V 1.392 0.99
65_A 208_A 1.391 0.99
199_A 203_L 1.389 0.99
114_L 142_A 1.385 0.99
141_S 145_I 1.379 0.99
113_F 120_L 1.363 0.99
159_L 200_G 1.353 0.99
42_N 241_P 1.342 0.99
87_V 90_L 1.334 0.99
174_A 188_I 1.323 0.99
61_M 225_L 1.31 0.99
140_P 223_N 1.301 0.99
163_I 189_I 1.301 0.99
15_L 19_M 1.298 0.99
166_L 192_L 1.285 0.99
58_S 227_G 1.281 0.99
168_K 171_L 1.28 0.99
25_F 38_F 1.253 0.99
19_M 23_T 1.252 0.99
74_Y 197_I 1.249 0.99
225_L 228_N 1.237 0.98
55_L 226_I 1.235 0.98
146_G 218_M 1.225 0.98
169_P 172_E 1.215 0.98
59_L 63_L 1.211 0.98
263_R 266_R 1.211 0.98
192_L 195_P 1.205 0.98
46_L 249_T 1.194 0.98
259_L 263_R 1.192 0.98
43_Y 245_A 1.187 0.98
179_A 184_T 1.186 0.98
122_E 126_W 1.171 0.98
121_N 124_L 1.164 0.97
92_L 160_Y 1.162 0.97
123_F 127_L 1.162 0.97
185_F 190_I 1.156 0.97
162_S 193_T 1.154 0.97
57_H 60_N 1.148 0.97
64_I 68_A 1.145 0.97
63_L 67_L 1.137 0.97
45_R 239_D 1.137 0.97
214_V 218_M 1.129 0.97
152_L 156_V 1.123 0.97
78_W 163_I 1.122 0.97
69_C 204_V 1.108 0.96
23_T 244_A 1.106 0.96
69_C 73_G 1.101 0.96
60_N 63_L 1.099 0.96
64_I 67_L 1.098 0.96
123_F 126_W 1.079 0.95
229_V 233_Q 1.073 0.95
233_Q 246_T 1.066 0.95
90_L 94_L 1.065 0.95
20_I 248_I 1.064 0.95
147_L 208_A 1.063 0.95
65_A 147_L 1.062 0.95
58_S 226_I 1.056 0.95
233_Q 239_D 1.053 0.95
229_V 246_T 1.049 0.94
186_I 190_I 1.044 0.94
75_P 79_F 1.042 0.94
143_V 215_S 1.041 0.94
78_W 185_F 1.038 0.94
211_L 215_S 1.033 0.94
224_L 228_N 1.033 0.94
194_M 197_I 1.032 0.94
67_L 71_V 1.03 0.94
47_L 52_F 1.03 0.94
122_E 125_L 1.027 0.94
233_Q 237_I 1.025 0.93
45_R 48_D 1.023 0.93
215_S 225_L 1.017 0.93
234_F 243_G 1.015 0.93
171_L 181_K 1.015 0.93
50_L 53_E 1.013 0.93
62_A 66_T 1.009 0.93
68_A 144_I 1.005 0.93
261_Y 264_A 1.001 0.92
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
2onkC40.921000.309Contact Map0.746
3d31C20.92731000.349Contact Map0.684
3rlfF10.97091000.389Contact Map0.653
3rlfG10.94911000.392Contact Map0.77
3tuiA40.763699.90.538Contact Map0.752
2rddB10.126.60.956Contact Map0.591
2kluA10.14916.60.956Contact Map0.172
2m20A20.13826.20.957Contact Map0.33
1vryA10.25454.10.96Contact Map0.207
2yevB20.27273.80.961Contact Map0.372
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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