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OPENSEQ.org
LACI - Lactose operon repressor
UniProt: P03023 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG10525
Length: 360 (331)
Sequences: 11583
Seq/Len: 34.99
LACI
Paralog alert: 0.90 [within 20: 0.27] - ratio of genomes with paralogs
Cluster includes: ALSB ARAF ASCG CRA CYTR DGAL EBGR GALR GALS GNTR IDNR LACI LSRB MALI PURR RBSB RBSR TORT TRER XYLF YCJW YPHF YTFQ
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
179_H 240_T 3.705 1.00
263_R 266_A 3.692 1.00
164_E 203_K 3.594 1.00
12_Y 44_E 3.583 1.00
170_G 271_V 3.235 1.00
275_D 290_K 2.919 1.00
179_H 269_S 2.779 1.00
40_A 44_E 2.774 1.00
168_R 207_R 2.596 1.00
32_A 36_E 2.571 1.00
257_I 268_I 2.565 1.00
198_L 202_H 2.537 1.00
36_E 40_A 2.521 1.00
82_A 297_G 2.351 1.00
157_N 307_L 2.342 1.00
172_E 176_A 2.224 1.00
23_V 38_V 2.21 1.00
86_R 302_D 2.178 1.00
35_R 39_E 2.167 1.00
167_T 171_V 2.148 1.00
121_G 304_L 2.105 1.00
226_F 259_E 2.06 1.00
234_N 260_S 2.054 1.00
299_T 302_D 2.034 1.00
24_V 42_M 2.005 1.00
175_V 208_N 1.981 1.00
167_T 243_L 1.924 1.00
263_R 267_D 1.917 1.00
177_L 329_T 1.908 1.00
168_R 203_K 1.903 1.00
172_E 208_N 1.902 1.00
290_K 322_S 1.888 1.00
87_A 301_V 1.872 1.00
165_D 169_L 1.87 1.00
262_L 267_D 1.863 1.00
230_M 260_S 1.857 1.00
225_G 252_G 1.846 1.00
7_Y 17_Y 1.808 1.00
9_V 41_A 1.801 1.00
285_P 327_K 1.791 1.00
86_R 89_Q 1.787 1.00
171_V 201_W 1.778 1.00
258_T 283_I 1.753 1.00
168_R 204_Y 1.752 1.00
168_R 172_E 1.742 1.00
166_G 273_Y 1.737 1.00
13_A 41_A 1.733 1.00
172_E 204_Y 1.722 1.00
85_S 294_R 1.718 1.00
226_F 256_A 1.701 1.00
171_V 175_V 1.697 1.00
64_I 87_A 1.684 1.00
230_M 256_A 1.664 1.00
229_T 242_M 1.664 1.00
173_H 329_T 1.663 1.00
229_T 253_A 1.654 1.00
23_V 42_M 1.645 1.00
39_E 43_A 1.641 1.00
275_D 326_R 1.626 1.00
39_E 42_M 1.62 1.00
292_D 295_L 1.605 1.00
9_V 13_A 1.605 1.00
172_E 207_R 1.604 1.00
200_G 204_Y 1.596 1.00
169_L 321_V 1.576 1.00
185_L 253_A 1.555 1.00
83_I 123_I 1.555 1.00
271_V 287_T 1.544 1.00
86_R 298_Q 1.544 1.00
86_R 301_V 1.541 1.00
82_A 294_R 1.529 1.00
162_S 165_D 1.514 1.00
164_E 199_A 1.509 1.00
12_Y 41_A 1.497 1.00
66_V 83_I 1.491 1.00
184_L 245_A 1.491 1.00
244_V 253_A 1.488 1.00
144_P 148_L 1.485 1.00
62_L 92_A 1.48 1.00
257_I 262_L 1.459 1.00
295_L 298_Q 1.456 1.00
256_A 259_E 1.455 1.00
175_V 204_Y 1.45 1.00
173_H 177_L 1.439 1.00
41_A 45_L 1.437 1.00
256_A 260_S 1.424 1.00
179_H 241_A 1.409 1.00
167_T 200_G 1.404 0.99
211_Q 214_A 1.403 0.99
254_M 270_V 1.402 0.99
50_N 53_A 1.397 0.99
295_L 299_T 1.389 0.99
31_S 34_T 1.376 0.99
171_V 200_G 1.368 0.99
12_Y 45_L 1.353 0.99
230_M 259_E 1.341 0.99
247_D 288_T 1.341 0.99
183_A 239_P 1.34 0.99
199_A 203_K 1.329 0.99
166_G 321_V 1.327 0.99
8_D 11_E 1.326 0.99
205_L 210_I 1.324 0.99
9_V 45_L 1.319 0.99
229_T 257_I 1.318 0.99
302_D 306_Q 1.317 0.99
173_H 325_K 1.31 0.99
6_L 20_V 1.308 0.99
90_L 305_L 1.304 0.99
182_I 205_L 1.29 0.99
254_M 264_V 1.281 0.99
87_A 94_V 1.278 0.99
174_L 241_A 1.278 0.99
50_N 56_L 1.272 0.99
171_V 204_Y 1.263 0.99
65_G 119_V 1.261 0.99
275_D 279_S 1.252 0.99
7_Y 11_E 1.248 0.99
34_T 37_K 1.242 0.98
182_I 241_A 1.237 0.98
269_S 329_T 1.226 0.98
306_Q 311_Q 1.211 0.98
175_V 210_I 1.208 0.98
292_D 319_L 1.207 0.98
183_A 232_M 1.206 0.98
62_L 120_S 1.2 0.98
6_L 17_Y 1.199 0.98
252_G 256_A 1.198 0.98
247_D 272_G 1.197 0.98
41_A 44_E 1.197 0.98
32_A 35_R 1.194 0.98
177_L 325_K 1.191 0.98
242_M 253_A 1.19 0.98
37_K 41_A 1.181 0.98
216_R 231_Q 1.178 0.98
61_S 120_S 1.174 0.98
254_M 282_Y 1.17 0.97
176_A 208_N 1.168 0.97
66_V 94_V 1.158 0.97
165_D 168_R 1.154 0.97
53_A 56_L 1.153 0.97
196_L 199_A 1.149 0.97
26_Q 35_R 1.143 0.97
297_G 301_V 1.139 0.97
272_G 288_T 1.138 0.97
86_R 90_L 1.134 0.97
236_G 239_P 1.132 0.97
66_V 80_V 1.13 0.97
148_L 300_S 1.13 0.97
40_A 43_A 1.118 0.96
36_E 39_E 1.117 0.96
181_Q 238_V 1.114 0.96
73_L 76_P 1.108 0.96
201_W 205_L 1.103 0.96
177_L 269_S 1.101 0.96
223_M 227_Q 1.096 0.96
22_R 27_A 1.094 0.96
4_V 45_L 1.094 0.96
23_V 30_V 1.091 0.96
182_I 201_W 1.076 0.95
203_K 207_R 1.076 0.95
227_Q 231_Q 1.064 0.95
204_Y 207_R 1.053 0.95
169_L 172_E 1.05 0.94
52_V 55_Q 1.046 0.94
13_A 20_V 1.044 0.94
170_G 243_L 1.042 0.94
244_V 250_A 1.042 0.94
164_E 200_G 1.037 0.94
173_H 176_A 1.031 0.94
161_F 292_D 1.029 0.94
85_S 298_Q 1.028 0.94
202_H 206_T 1.026 0.94
42_M 45_L 1.017 0.93
97_S 110_A 1.016 0.93
180_Q 240_T 1.014 0.93
225_G 229_T 1.012 0.93
97_S 114_L 1.008 0.93
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
1jyeA10.96671000.166Contact Map0.762
3dbiA30.92221000.181Contact Map0.747
2o20A80.90561000.208Contact Map0.848
1qpzA10.92781000.209Contact Map0.769
2hsgA10.90831000.209Contact Map0.763
3e3mA40.93611000.215Contact Map0.817
3bilA20.93891000.216Contact Map0.658
3kjxA40.91941000.218Contact Map0.787
3h5oA20.92781000.219Contact Map0.791
3ctpA20.91111000.223Contact Map0.743
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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