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OPENSEQ.org

RLUE - Ribosomal large subunit pseudouridine synthase E
UniProt: P75966 Sequence
Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG13447
Length: 217 (183)
Sequences: 1898
Seq/Len: 10.37

RLUE
Paralog alert: 0.45 [within 20: 0.00] - ratio of genomes with paralogs
Cluster includes: RLUA RLUB RLUC RLUD RLUE RLUF RSUA TRUC
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
142_E 169_K 3.534 1.00
44_F 66_F 3.032 1.00
40_R 214_E 2.612 1.00
123_E 127_N 2.602 1.00
106_G 171_T 2.553 1.00
178_R 181_R 2.545 1.00
56_D 60_R 2.54 1.00
44_F 67_I 2.531 1.00
121_A 189_F 2.364 1.00
108_I 198_A 2.359 1.00
50_V 66_F 2.312 1.00
208_A 211_E 2.048 1.00
131_L 182_R 2.005 1.00
49_D 80_R 1.958 1.00
122_L 126_R 1.874 1.00
131_L 134_G 1.872 1.00
143_L 166_S 1.845 1.00
141_A 170_I 1.736 1.00
179_Q 183_M 1.726 1.00
117_P 166_S 1.725 1.00
43_L 202_Y 1.706 1.00
108_I 169_K 1.63 1.00
106_G 140_G 1.582 1.00
98_L 199_M 1.582 1.00
60_R 80_R 1.581 1.00
130_T 135_P 1.578 1.00
42_I 212_W 1.566 1.00
83_E 193_R 1.514 1.00
125_L 187_V 1.508 1.00
89_T 95_Q 1.505 1.00
121_A 187_V 1.497 1.00
42_I 214_E 1.487 1.00
123_E 126_R 1.485 1.00
141_A 168_L 1.464 1.00
95_Q 99_T 1.462 1.00
83_E 195_I 1.46 1.00
110_Y 197_Y 1.455 1.00
203_S 206_N 1.449 1.00
136_T 139_A 1.417 1.00
135_P 176_R 1.415 1.00
63_L 86_L 1.393 0.99
45_N 151_W 1.389 0.99
129_V 187_V 1.374 0.99
134_G 182_R 1.369 0.99
133_D 182_R 1.362 0.99
82_S 181_R 1.36 0.99
106_G 173_Y 1.335 0.99
110_Y 144_V 1.326 0.99
73_Y 95_Q 1.314 0.99
183_M 187_V 1.313 0.99
121_A 124_A 1.308 0.99
52_P 86_L 1.294 0.99
118_T 121_A 1.291 0.99
136_T 179_Q 1.261 0.99
125_L 129_V 1.26 0.99
108_I 171_T 1.259 0.99
112_Q 165_T 1.25 0.99
41_V 91_N 1.243 0.98
48_Y 83_E 1.237 0.98
96_A 99_T 1.227 0.98
61_K 65_E 1.22 0.98
139_A 179_Q 1.216 0.98
167_W 195_I 1.212 0.98
44_F 50_V 1.209 0.98
96_A 100_Q 1.175 0.98
111_V 168_L 1.17 0.97
110_Y 169_K 1.153 0.97
82_S 180_V 1.147 0.97
114_E 190_P 1.146 0.97
144_V 169_K 1.14 0.97
99_T 199_M 1.138 0.97
125_L 183_M 1.137 0.97
56_D 80_R 1.136 0.97
139_A 172_L 1.119 0.96
42_I 72_V 1.118 0.96
43_L 213_R 1.118 0.96
168_L 184_T 1.11 0.96
117_P 187_V 1.097 0.96
106_G 198_A 1.091 0.96
56_D 62_T 1.09 0.96
134_G 176_R 1.088 0.96
54_F 64_K 1.084 0.96
54_F 74_A 1.081 0.95
85_L 204_L 1.077 0.95
53_Q 62_T 1.077 0.95
138_P 141_A 1.055 0.95
49_D 61_K 1.051 0.94
112_Q 193_R 1.051 0.94
111_V 180_V 1.043 0.94
122_L 141_A 1.039 0.94
116_I 166_S 1.035 0.94
184_T 191_T 1.03 0.94
119_Q 123_E 1.025 0.93
39_T 91_N 1.016 0.93
81_D 181_R 1.004 0.93
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
2olwA211000.422Contact Map0.804
2gmlA20.79261000.468Contact Map0.716
2omlA10.8711000.501Contact Map0.77
3dh3A40.90781000.52Contact Map0.73
4lgtA20.93091000.539Contact Map0.819
1vioA20.90781000.556Contact Map0.773
1kskA10.90781000.567Contact Map0.685
2i82A40.83871000.661Contact Map0.481
1v9fA10.926399.90.687Contact Map0.5
1v9kA20.778899.90.695Contact Map0.474

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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