May 4, 2021 - We are working on upgrading the webserver, some pages may not work.
OPENSEQ.org

RLUF - Ribosomal large subunit pseudouridine synthase F
UniProt: P32684 Sequence
Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG11921
Length: 290 (233)
Sequences: 3056
Seq/Len: 13.12

RLUF
Paralog alert: 0.67 [within 20: 0.01] - ratio of genomes with paralogs
Cluster includes: RLUA RLUB RLUC RLUD RLUE RLUF RSUA TRUC
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
36_N 50_D 5.764 1.00
32_N 55_N 5.143 1.00
34_F 53_K 3.919 1.00
170_K 178_R 3.249 1.00
136_E 207_R 2.821 1.00
72_L 93_F 2.688 1.00
19_C 24_A 2.465 1.00
187_R 190_R 2.356 1.00
12_Y 46_V 2.354 1.00
27_Y 54_V 2.11 1.00
159_I 191_R 2.103 1.00
151_R 155_A 2.1 1.00
188_Q 192_M 2.087 1.00
26_R 30_Q 2.072 1.00
111_Q 202_K 2.071 1.00
10_N 25_D 2.056 1.00
111_Q 204_E 2.055 1.00
40_A 44_D 2.042 1.00
70_I 223_D 2.021 1.00
222_R 228_E 1.964 1.00
70_I 221_W 1.922 1.00
149_F 198_Y 1.913 1.00
138_L 178_R 1.884 1.00
217_P 220_E 1.879 1.00
51_V 58_L 1.849 1.00
101_F 123_V 1.843 1.00
226_D 230_I 1.822 1.00
78_I 93_F 1.799 1.00
13_I 24_A 1.746 1.00
136_E 178_R 1.737 1.00
12_Y 52_V 1.727 1.00
134_E 180_T 1.717 1.00
87_R 108_K 1.708 1.00
140_T 176_V 1.691 1.00
111_Q 218_L 1.69 1.00
34_F 39_R 1.68 1.00
150_I 154_S 1.68 1.00
53_K 58_L 1.656 1.00
153_M 169_V 1.569 1.00
136_E 180_T 1.563 1.00
87_R 90_I 1.53 1.00
13_I 59_I 1.521 1.00
151_R 154_S 1.513 1.00
71_A 211_V 1.512 1.00
134_E 207_R 1.51 1.00
153_M 196_F 1.509 1.00
177_F 193_C 1.507 1.00
134_E 168_K 1.462 1.00
142_D 199_E 1.455 1.00
23_E 26_R 1.424 1.00
76_V 111_Q 1.42 1.00
33_V 54_V 1.399 0.99
82_T 102_P 1.377 0.99
71_A 113_L 1.352 0.99
28_I 33_V 1.35 0.99
90_I 114_I 1.341 0.99
172_E 178_R 1.332 0.99
158_P 163_V 1.329 0.99
123_V 127_L 1.323 0.99
141_V 198_Y 1.313 0.99
110_S 189_I 1.31 0.99
173_A 176_V 1.308 0.99
138_L 176_V 1.284 0.99
169_V 177_F 1.282 0.99
146_T 149_F 1.278 0.99
140_T 202_K 1.261 0.99
69_L 119_H 1.258 0.99
126_I 208_I 1.253 0.99
34_F 56_G 1.249 0.99
26_R 29_E 1.249 0.99
153_M 193_C 1.238 0.98
68_V 223_D 1.236 0.98
139_V 177_F 1.233 0.98
134_E 182_V 1.231 0.98
7_V 12_Y 1.229 0.98
169_V 179_I 1.225 0.98
87_R 91_V 1.223 0.98
110_S 139_V 1.212 0.98
143_K 198_Y 1.207 0.98
82_T 91_V 1.204 0.98
127_L 208_I 1.204 0.98
13_I 19_C 1.199 0.98
8_R 45_Q 1.192 0.98
29_E 42_I 1.186 0.98
164_T 167_C 1.171 0.98
110_S 190_R 1.171 0.98
80_S 102_P 1.142 0.97
158_P 195_H 1.139 0.97
149_F 196_F 1.13 0.97
207_R 212_S 1.128 0.97
46_V 52_V 1.121 0.97
164_T 188_Q 1.118 0.96
125_K 131_N 1.117 0.96
41_T 44_D 1.116 0.96
7_V 15_E 1.112 0.96
117_T 123_V 1.109 0.96
80_S 91_V 1.105 0.96
35_L 44_D 1.103 0.96
225_T 228_E 1.099 0.96
100_V 116_L 1.098 0.96
147_E 151_R 1.098 0.96
124_N 128_R 1.096 0.96
161_G 191_R 1.089 0.96
194_E 199_E 1.081 0.95
210_N 231_D 1.08 0.95
181_L 188_Q 1.078 0.95
109_D 190_R 1.077 0.95
124_N 127_L 1.076 0.95
113_L 213_L 1.071 0.95
138_L 206_T 1.058 0.95
9_L 28_I 1.056 0.95
222_R 225_T 1.048 0.94
141_V 193_C 1.047 0.94
18_I 54_V 1.041 0.94
122_L 208_I 1.039 0.94
121_D 125_K 1.031 0.94
94_V 116_L 1.023 0.93
153_M 192_M 1.019 0.93
69_L 122_L 1.018 0.93
149_F 152_G 1.006 0.93
88_D 91_V 1 0.92
145_I 177_F 1 0.92
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
3dh3A411000.231Contact Map0.874
4lgtA20.83451000.366Contact Map0.835
1vioA20.81721000.372Contact Map0.845
1kskA10.78621000.378Contact Map0.755
2gmlA20.81381000.382Contact Map0.788
2olwA20.65861000.524Contact Map0.831
2omlA10.58281000.525Contact Map0.802
1v9fA10.94141000.527Contact Map0.526
2i82A40.6691000.646Contact Map0.497
1v9kA20.68281000.663Contact Map0.484

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

Page generated in 0.0505 seconds.