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OPENSEQ.org

RLUA - Ribosomal large subunit pseudouridine synthase A
UniProt: P0AA37 Sequence
Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG12609
Length: 219 (207)
Sequences: 5925
Seq/Len: 28.62

RLUA
Paralog alert: 0.91 [within 20: 0.03] - ratio of genomes with paralogs
Cluster includes: RLUA RLUB RLUC RLUD RLUE RLUF RSUA TRUC
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
95_Q 153_V 3.594 1.00
138_E 157_I 3.528 1.00
17_L 70_V 3.381 1.00
19_Q 75_L 3.328 1.00
199_M 212_T 3.038 1.00
201_T 212_T 3.009 1.00
140_E 153_V 2.9 1.00
110_L 138_E 2.814 1.00
22_H 76_T 2.812 1.00
15_V 27_N 2.784 1.00
154_L 168_M 2.713 1.00
97_V 198_E 2.587 1.00
154_L 164_L 2.53 1.00
22_H 206_A 2.475 1.00
109_G 139_Y 2.46 1.00
97_V 151_R 2.436 1.00
25_V 213_F 2.406 1.00
76_T 79_A 2.346 1.00
110_L 136_Q 2.269 1.00
113_L 170_A 2.231 1.00
27_N 215_A 2.199 1.00
107_A 144_Y 2.188 1.00
203_T 210_S 2.182 1.00
201_T 210_S 2.102 1.00
68_S 196_H 2.073 1.00
112_D 136_Q 2.043 1.00
141_V 150_A 2.012 1.00
199_M 214_K 1.991 1.00
136_Q 157_I 1.981 1.00
86_Q 91_E 1.941 1.00
93_K 155_K 1.939 1.00
108_E 140_E 1.894 1.00
100_V 152_V 1.841 1.00
20_D 204_H 1.836 1.00
85_R 89_E 1.832 1.00
137_T 154_L 1.831 1.00
104_P 150_A 1.789 1.00
95_Q 155_K 1.786 1.00
98_A 154_L 1.763 1.00
95_Q 199_M 1.73 1.00
93_K 201_T 1.723 1.00
112_D 134_P 1.69 1.00
142_V 153_V 1.675 1.00
25_V 72_V 1.657 1.00
102_G 150_A 1.651 1.00
63_L 71_I 1.64 1.00
152_V 168_M 1.586 1.00
46_V 71_I 1.536 1.00
97_V 153_V 1.506 1.00
190_A 194_L 1.502 1.00
20_D 23_I 1.493 1.00
34_S 71_I 1.483 1.00
100_V 168_M 1.466 1.00
138_E 155_K 1.464 1.00
94_K 156_P 1.457 1.00
66_A 193_L 1.453 1.00
82_E 86_Q 1.449 1.00
25_V 70_V 1.412 1.00
80_E 84_K 1.41 1.00
141_V 144_Y 1.356 0.99
198_E 214_K 1.325 0.99
60_V 74_A 1.31 0.99
111_V 171_L 1.295 0.99
204_H 211_M 1.286 0.99
60_V 83_L 1.285 0.99
86_Q 203_T 1.268 0.99
115_L 166_V 1.251 0.99
44_D 49_R 1.243 0.98
168_M 175_I 1.231 0.98
110_L 157_I 1.23 0.98
137_T 167_H 1.225 0.98
23_I 202_I 1.219 0.98
45_S 48_T 1.216 0.98
82_E 85_R 1.192 0.98
17_L 213_F 1.175 0.98
192_R 196_H 1.167 0.97
30_S 192_R 1.167 0.97
29_P 32_L 1.158 0.97
98_A 175_I 1.157 0.97
85_R 88_R 1.156 0.97
30_S 68_S 1.153 0.97
99_R 149_T 1.153 0.97
128_C 131_T 1.147 0.97
70_V 213_F 1.129 0.97
97_V 217_A 1.121 0.97
203_T 208_G 1.112 0.96
50_I 73_V 1.111 0.96
156_P 164_L 1.111 0.96
103_H 144_Y 1.109 0.96
133_K 160_R 1.095 0.96
87_F 92_P 1.091 0.96
196_H 215_A 1.085 0.96
97_V 142_V 1.078 0.95
102_G 173_H 1.071 0.95
81_R 85_R 1.056 0.95
197_A 200_L 1.053 0.95
121_N 124_K 1.04 0.94
23_I 204_H 1.03 0.94
78_A 206_A 1.028 0.94
115_L 125_Q 1.024 0.93
48_T 51_Q 1.015 0.93
149_T 176_L 1.012 0.93
104_P 173_H 1.008 0.93
87_F 156_P 1.006 0.93
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
2i82A40.99091000.111Contact Map0.915
1v9fA10.97721000.15Contact Map0.901
1v9kA20.93151000.158Contact Map0.862
2olwA20.88131000.376Contact Map0.646
2omlA10.79911000.383Contact Map0.672
4lgtA20.89041000.423Contact Map0.532
1vioA20.87671000.451Contact Map0.628
3dh3A40.82191000.454Contact Map0.483
1kskA10.84931000.46Contact Map0.579
2gmlA20.87211000.466Contact Map0.698

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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