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OPENSEQ.org

RSUA - Ribosomal small subunit pseudouridine synthase A
UniProt: P0AA43 Sequence
Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG12044
Length: 231 (229)
Sequences: 7074
Seq/Len: 30.89

RSUA
Paralog alert: 0.95 [within 20: 0.07] - ratio of genomes with paralogs
Cluster includes: RLUA RLUB RLUC RLUD RLUE RLUF RSUA TRUC
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
30_D 45_H 5.175 1.00
131_T 175_R 4.124 1.00
55_A 108_L 3.712 1.00
167_E 175_R 3.711 1.00
28_T 48_A 3.233 1.00
26_R 50_D 2.965 1.00
165_V 179_S 2.883 1.00
58_H 113_D 2.593 1.00
61_R 114_D 2.514 1.00
129_E 177_T 2.319 1.00
83_V 109_V 2.318 1.00
133_L 173_Q 2.302 1.00
34_V 39_F 2.189 1.00
169_I 175_R 2.048 1.00
176_L 190_F 2.019 1.00
133_L 175_R 2.012 1.00
131_T 177_T 1.988 1.00
56_Q 59_G 1.903 1.00
174_V 190_F 1.889 1.00
6_F 41_L 1.875 1.00
20_R 24_G 1.868 1.00
147_Q 192_A 1.863 1.00
13_V 18_A 1.807 1.00
184_H 187_K 1.806 1.00
215_A 218_E 1.801 1.00
29_V 39_F 1.793 1.00
21_E 49_Y 1.793 1.00
101_L 109_V 1.776 1.00
71_Y 86_F 1.767 1.00
131_T 204_R 1.753 1.00
7_I 18_A 1.725 1.00
124_P 127_H 1.713 1.00
114_D 117_W 1.677 1.00
65_L 86_F 1.661 1.00
73_C 109_V 1.659 1.00
163_P 179_S 1.647 1.00
224_E 228_A 1.638 1.00
3_L 34_V 1.627 1.00
69_Q 106_T 1.607 1.00
17_I 20_R 1.599 1.00
134_V 176_L 1.577 1.00
135_T 173_Q 1.574 1.00
106_T 199_E 1.569 1.00
130_K 178_I 1.564 1.00
220_R 226_E 1.562 1.00
185_Q 189_M 1.55 1.00
63_F 221_P 1.521 1.00
146_E 163_P 1.504 1.00
118_S 122_T 1.491 1.00
140_V 172_T 1.487 1.00
96_H 118_S 1.466 1.00
168_V 172_T 1.456 1.00
136_L 195_N 1.453 1.00
176_L 186_V 1.452 1.00
164_A 176_L 1.434 1.00
154_L 188_R 1.432 1.00
146_E 161_T 1.421 1.00
164_A 178_I 1.4 0.99
136_L 190_F 1.375 0.99
165_V 177_T 1.374 0.99
28_T 33_I 1.372 0.99
120_R 124_P 1.359 0.99
29_V 47_V 1.354 0.99
63_F 219_Y 1.352 0.99
149_A 203_E 1.341 0.99
170_T 173_Q 1.341 0.99
64_M 110_L 1.338 0.99
106_T 201_H 1.314 0.99
134_V 174_V 1.296 0.99
22_I 27_V 1.288 0.99
106_T 216_P 1.285 0.99
136_L 174_V 1.282 0.99
178_I 186_V 1.268 0.99
4_D 19_G 1.26 0.99
62_Y 117_W 1.249 0.99
138_S 195_N 1.248 0.99
27_V 49_Y 1.229 0.98
119_H 123_S 1.227 0.98
6_F 10_Q 1.219 0.98
134_V 197_V 1.212 0.98
166_L 190_F 1.199 0.98
155_H 158_K 1.194 0.98
64_M 108_L 1.185 0.98
31_G 48_A 1.175 0.98
73_C 97_A 1.175 0.98
3_L 22_I 1.173 0.98
98_A 112_T 1.172 0.98
75_T 84_L 1.154 0.97
17_I 21_E 1.126 0.97
41_L 47_V 1.121 0.97
23_R 37_A 1.121 0.97
167_E 177_T 1.11 0.96
138_S 172_T 1.107 0.96
42_L 45_H 1.105 0.96
8_A 15_R 1.093 0.96
87_L 111_M 1.087 0.96
116_Q 120_R 1.076 0.95
140_V 174_V 1.071 0.95
64_M 208_I 1.058 0.95
59_G 62_Y 1.057 0.95
68_P 71_Y 1.053 0.95
129_E 204_R 1.053 0.95
13_V 21_E 1.048 0.94
166_L 176_L 1.043 0.94
120_R 123_S 1.035 0.94
137_E 198_V 1.029 0.94
207_G 229_S 1.028 0.94
19_G 23_R 1.024 0.93
220_R 223_T 1.023 0.93
130_K 179_S 1.02 0.93
223_T 226_E 1.015 0.93
75_T 82_T 1.009 0.93
15_R 19_G 1.009 0.93
29_V 34_V 1.008 0.93
7_I 27_V 1.008 0.93
133_L 169_I 1.007 0.93
149_A 152_V 1.006 0.93
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
1vioA20.9871000.101Contact Map0.775
1kskA10.9871000.129Contact Map0.684
3dh3A40.96971000.135Contact Map0.81
4lgtA20.9611000.145Contact Map0.721
1v9fA10.9871000.211Contact Map0.846
2gmlA20.77921000.261Contact Map0.786
2omlA10.72731000.283Contact Map0.734
2olwA20.81391000.286Contact Map0.796
1v9kA20.77061000.377Contact Map0.773
2i82A40.80951000.397Contact Map0.816

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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