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OPENSEQ.org
HTRE - Outer membrane usher protein HtrE
UniProt: P33129 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG11972
Length: 865 (804)
Sequences: 1302
Seq/Len: 1.62
HTRE
Paralog alert: 0.81 [within 20: 0.05] - ratio of genomes with paralogs
Cluster includes: ELFC FIMD HTRE SFMD YAGX YBGQ YEHB YFCU YHCD YQIG YRAJ
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
94_C 127_C 4.281 1.00
287_I 299_I 4.004 1.00
701_A 731_L 3.862 1.00
659_A 675_S 3.759 1.00
295_A 336_E 3.755 1.00
690_S 727_V 3.237 1.00
251_E 265_R 3.17 1.00
797_I 825_I 3.009 1.00
360_G 387_Y 2.897 1.00
779_A 819_I 2.885 1.00
264_I 380_L 2.879 1.00
688_T 721_D 2.876 1.00
296_K 335_E 2.725 1.00
661_S 673_S 2.715 1.00
823_G 842_Y 2.659 1.00
333_T 341_K 2.651 1.00
292_N 340_S 2.648 1.00
704_Q 770_D 2.625 1.00
682_L 687_L 2.509 1.00
335_E 341_K 2.504 1.00
680_F 689_F 2.492 1.00
522_G 547_Y 2.484 1.00
360_G 385_Y 2.455 1.00
292_N 342_R 2.39 1.00
628_S 648_Y 2.389 1.00
683_H 688_T 2.382 1.00
334_I 342_R 2.363 1.00
797_I 814_A 2.357 1.00
331_I 343_T 2.323 1.00
795_A 827_I 2.32 1.00
210_A 238_R 2.311 1.00
431_V 477_A 2.276 0.99
300_T 305_K 2.274 0.99
702_V 725_Y 2.263 0.99
183_L 200_A 2.259 0.99
299_I 318_I 2.252 0.99
630_N 646_G 2.187 0.99
217_G 229_Y 2.15 0.99
783_I 840_A 2.14 0.99
176_A 766_V 2.113 0.99
549_N 556_Y 2.097 0.99
69_Y 147_R 2.053 0.99
213_L 236_V 2.052 0.99
600_T 612_L 2.038 0.99
82_F 93_A 2.035 0.99
205_G 214_R 2.019 0.98
679_G 690_S 1.998 0.98
66_V 82_F 1.992 0.98
174_I 702_V 1.982 0.98
396_Y 425_D 1.981 0.98
425_D 445_R 1.981 0.98
66_V 141_Y 1.976 0.98
525_Y 546_G 1.949 0.98
300_T 333_T 1.94 0.98
704_Q 768_F 1.906 0.98
658_L 676_T 1.884 0.97
700_L 766_V 1.881 0.97
685_G 704_Q 1.865 0.97
298_T 305_K 1.858 0.97
259_F 398_G 1.83 0.97
679_G 693_S 1.793 0.96
683_H 725_Y 1.76 0.96
443_S 467_S 1.76 0.96
164_Y 167_P 1.758 0.95
737_N 761_P 1.736 0.95
129_N 132_E 1.734 0.95
237_Q 246_Q 1.731 0.95
213_L 234_R 1.712 0.95
825_I 840_A 1.712 0.95
777_Q 821_Q 1.707 0.94
656_G 676_T 1.703 0.94
698_D 763_Q 1.689 0.94
705_A 773_T 1.683 0.94
268_R 363_D 1.678 0.94
364_I 383_A 1.672 0.94
705_A 708_A 1.661 0.93
106_N 109_D 1.655 0.93
248_I 270_Y 1.639 0.93
559_S 575_Y 1.635 0.93
151_D 312_P 1.633 0.93
140_R 149_D 1.619 0.92
298_T 335_E 1.61 0.92
206_M 213_L 1.599 0.92
240_I 243_L 1.598 0.91
443_S 465_R 1.59 0.91
127_C 354_M 1.588 0.91
162_Q 322_S 1.577 0.91
783_I 814_A 1.568 0.90
287_I 330_L 1.565 0.90
49_D 53_Y 1.564 0.90
457_T 466_Y 1.559 0.90
680_F 687_L 1.558 0.90
274_R 763_Q 1.553 0.90
252_S 264_I 1.552 0.90
797_I 827_I 1.552 0.90
334_I 344_F 1.551 0.90
273_S 361_R 1.55 0.90
175_N 206_M 1.542 0.89
693_S 700_L 1.541 0.89
212_R 762_R 1.535 0.89
749_D 777_Q 1.531 0.89
684_S 768_F 1.53 0.89
94_C 354_M 1.527 0.89
252_S 365_S 1.524 0.89
442_Q 468_T 1.519 0.88
273_S 359_V 1.519 0.88
346_Q 354_M 1.517 0.88
443_S 534_W 1.513 0.88
68_V 80_I 1.513 0.88
139_V 150_I 1.513 0.88
331_I 345_S 1.512 0.88
783_I 827_I 1.51 0.88
679_G 700_L 1.504 0.88
791_I 827_I 1.501 0.88
679_G 727_V 1.499 0.88
548_S 557_S 1.483 0.87
96_T 99_N 1.481 0.87
514_L 522_G 1.477 0.87
761_P 767_V 1.47 0.86
428_H 442_Q 1.468 0.86
288_H 317_V 1.464 0.86
701_A 739_I 1.455 0.85
408_A 427_T 1.454 0.85
65_D 79_S 1.453 0.85
653_S 656_G 1.448 0.85
172_N 241_A 1.441 0.85
785_R 791_I 1.438 0.85
505_Q 531_S 1.436 0.84
711_A 720_I 1.423 0.84
284_A 356_R 1.421 0.84
708_A 773_T 1.417 0.83
63_V 79_S 1.415 0.83
599_D 615_S 1.4 0.82
291_A 311_V 1.398 0.82
183_L 198_I 1.397 0.82
260_D 473_G 1.392 0.82
179_L 204_G 1.391 0.82
264_I 365_S 1.391 0.82
562_R 572_D 1.387 0.81
178_M 700_L 1.383 0.81
239_D 246_Q 1.377 0.81
215_A 231_F 1.368 0.80
61_P 644_Y 1.366 0.80
207_N 214_R 1.363 0.80
278_P 735_H 1.356 0.79
785_R 789_K 1.353 0.79
526_L 545_I 1.341 0.78
94_C 346_Q 1.34 0.78
681_V 700_L 1.339 0.78
69_Y 74_P 1.337 0.78
352_V 380_L 1.332 0.78
239_D 762_R 1.331 0.78
736_E 758_V 1.328 0.77
682_L 702_V 1.32 0.77
523_S 814_A 1.319 0.77
792_P 795_A 1.317 0.77
681_V 702_V 1.298 0.75
779_A 844_Q 1.298 0.75
691_N 717_N 1.295 0.75
726_G 729_S 1.292 0.75
185_G 674_L 1.291 0.75
781_M 858_N 1.286 0.74
681_V 766_V 1.285 0.74
294_N 336_E 1.284 0.74
693_S 766_V 1.283 0.74
685_G 768_F 1.282 0.74
357_P 391_N 1.28 0.74
393_L 415_L 1.276 0.73
721_D 727_V 1.274 0.73
332_V 346_Q 1.273 0.73
503_K 534_W 1.271 0.73
551_T 556_Y 1.269 0.73
285_P 388_G 1.269 0.73
174_I 766_V 1.268 0.73
838_C 862_C 1.268 0.73
705_A 711_A 1.268 0.73
459_L 509_S 1.266 0.73
706_P 720_I 1.264 0.72
99_N 103_F 1.263 0.72
264_I 268_R 1.262 0.72
265_R 370_L 1.261 0.72
175_N 684_S 1.261 0.72
752_L 755_T 1.261 0.72
298_T 308_E 1.26 0.72
334_I 346_Q 1.259 0.72
400_Q 474_L 1.256 0.72
215_A 234_R 1.244 0.71
389_L 780_I 1.244 0.71
175_N 766_V 1.243 0.71
605_D 608_G 1.242 0.70
653_S 658_L 1.241 0.70
683_H 686_G 1.236 0.70
296_K 308_E 1.233 0.70
604_S 609_N 1.231 0.69
713_I 728_T 1.231 0.69
776_G 818_G 1.228 0.69
455_T 512_Q 1.227 0.69
720_I 778_S 1.224 0.69
181_Y 678_G 1.223 0.69
508_V 526_L 1.22 0.68
704_Q 725_Y 1.215 0.68
824_N 839_L 1.212 0.68
368_Q 379_N 1.208 0.67
530_W 541_S 1.207 0.67
300_T 331_I 1.206 0.67
127_C 346_Q 1.204 0.67
688_T 723_W 1.199 0.67
100_L 105_I 1.199 0.67
706_P 723_W 1.196 0.66
69_Y 149_D 1.195 0.66
248_I 252_S 1.187 0.65
174_I 684_S 1.182 0.65
285_P 420_G 1.18 0.65
792_P 829_W 1.177 0.64
142_D 149_D 1.177 0.64
783_I 791_I 1.176 0.64
67_S 70_V 1.176 0.64
313_P 608_G 1.171 0.64
295_A 338_D 1.169 0.64
203_N 216_S 1.167 0.63
388_G 420_G 1.166 0.63
420_G 448_W 1.161 0.63
556_Y 576_L 1.16 0.63
70_V 157_V 1.158 0.63
235_Y 764_G 1.157 0.62
349_S 354_M 1.157 0.62
783_I 808_V 1.156 0.62
388_G 394_T 1.155 0.62
396_Y 445_R 1.153 0.62
67_S 77_N 1.15 0.62
177_A 206_M 1.15 0.62
100_L 471_Y 1.147 0.61
157_V 267_I 1.146 0.61
688_T 702_V 1.144 0.61
799_D 803_N 1.142 0.61
94_C 699_T 1.142 0.61
443_S 464_Y 1.141 0.61
795_A 829_W 1.138 0.61
509_S 577_S 1.135 0.60
781_M 816_V 1.135 0.60
333_T 343_T 1.129 0.60
249_L 267_I 1.129 0.60
357_P 390_N 1.129 0.60
173_G 301_Q 1.128 0.60
831_E 836_V 1.126 0.59
239_D 346_Q 1.124 0.59
514_L 547_Y 1.121 0.59
239_D 734_Y 1.121 0.59
728_T 739_I 1.117 0.58
369_V 478_L 1.117 0.58
602_I 610_N 1.116 0.58
238_R 247_L 1.116 0.58
318_I 729_S 1.11 0.58
59_A 143_V 1.109 0.58
778_S 817_R 1.108 0.58
840_A 860_I 1.108 0.58
141_Y 146_Q 1.106 0.57
386_Y 778_S 1.104 0.57
504_N 532_D 1.099 0.57
134_I 137_A 1.099 0.57
683_H 702_V 1.099 0.57
245_S 362_W 1.098 0.56
316_F 336_E 1.097 0.56
460_N 464_Y 1.096 0.56
574_V 602_I 1.096 0.56
431_V 472_L 1.088 0.55
170_W 760_V 1.085 0.55
703_V 827_I 1.082 0.55
425_D 443_S 1.082 0.55
134_I 156_W 1.081 0.55
177_A 682_L 1.081 0.55
530_W 539_N 1.08 0.55
762_R 765_S 1.08 0.55
688_T 725_Y 1.08 0.55
620_S 627_Y 1.077 0.54
796_D 804_V 1.075 0.54
343_T 574_V 1.075 0.54
451_L 456_S 1.073 0.54
605_D 609_N 1.069 0.53
159_K 319_D 1.069 0.53
150_I 195_N 1.069 0.53
426_V 524_F 1.063 0.53
231_F 701_A 1.061 0.53
776_G 815_F 1.061 0.53
561_Q 573_S 1.061 0.53
178_M 766_V 1.061 0.53
831_E 834_K 1.061 0.53
110_I 156_W 1.06 0.53
105_I 134_I 1.059 0.52
469_Q 739_I 1.058 0.52
281_A 735_H 1.056 0.52
649_A 660_G 1.055 0.52
455_T 462_A 1.054 0.52
245_S 271_S 1.048 0.51
455_T 528_G 1.048 0.51
389_L 395_G 1.047 0.51
532_D 539_N 1.046 0.51
352_V 449_N 1.044 0.51
460_N 507_T 1.043 0.51
788_G 855_I 1.042 0.51
260_D 396_Y 1.036 0.50
240_I 269_L 1.036 0.50
664_A 670_R 1.036 0.50
231_F 251_E 1.032 0.50
615_S 646_G 1.032 0.50
515_K 520_D 1.029 0.49
239_D 342_R 1.029 0.49
132_E 299_I 1.029 0.49
322_S 675_S 1.029 0.49
169_L 400_Q 1.029 0.49
683_H 721_D 1.028 0.49
154_Q 282_S 1.028 0.49
61_P 341_K 1.027 0.49
810_Q 817_R 1.026 0.49
262_V 400_Q 1.026 0.49
212_R 765_S 1.025 0.49
70_V 135_P 1.025 0.49
524_F 599_D 1.023 0.49
513_P 605_D 1.021 0.48
447_S 460_N 1.017 0.48
170_W 793_F 1.016 0.48
178_M 681_V 1.016 0.48
443_S 462_A 1.014 0.48
855_I 858_N 1.014 0.48
281_A 380_L 1.013 0.48
260_D 443_S 1.012 0.47
652_E 691_N 1.011 0.47
156_W 315_A 1.011 0.47
293_T 408_A 1.01 0.47
146_Q 355_L 1.008 0.47
101_L 158_M 1.007 0.47
63_V 81_T 1.007 0.47
457_T 512_Q 1.005 0.47
59_A 91_A 1.005 0.47
823_G 844_Q 1.002 0.46
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
3rfzB20.93181000.068Contact Map0.708
3fipA20.55031000.457Contact Map0.724
3fcgA20.1041000.834Contact Map0.782
4b0mA10.15381000.85Contact Map0.836
3bwuD10.144599.90.865Contact Map0.846
2xetA20.100699.70.898Contact Map0.834
3l48A50.100699.70.903Contact Map0.932
3e8vA10.086767.20.978Contact Map0.435
4kdwA10.130664.90.978Contact Map0.493
3pe9A40.107530.30.982Contact Map0.705
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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