May 4, 2021 - We are working on upgrading the webserver, some pages may not work.
OPENSEQ.org
MURJ - Probable peptidoglycan biosynthesis protein MurJ
UniProt: P0AF16 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG13880
Length: 511 (486)
Sequences: 13364
Seq/Len: 27.50
MURJ
Paralog alert: 0.92 [within 20: 0.16] - ratio of genomes with paralogs
Cluster includes: DINF MDTK MURJ RFBX WZXC WZXE YEEO YGHQ
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
76_G 79_A 4.501 1.00
299_N 302_E 4.012 1.00
227_M 231_K 3.708 1.00
156_R 159_I 3.43 1.00
105_A 108_W 3.351 1.00
42_F 266_Y 3.267 1.00
140_L 198_V 3.206 1.00
328_L 331_P 2.82 1.00
333_T 350_T 2.82 1.00
128_S 132_K 2.748 1.00
129_Q 187_L 2.632 1.00
69_A 292_S 2.572 1.00
107_P 128_S 2.424 1.00
329_S 355_I 2.416 1.00
329_S 351_Q 2.387 1.00
406_K 409_G 2.339 1.00
64_F 87_V 2.263 1.00
223_D 227_M 2.252 1.00
379_D 382_T 2.128 1.00
264_W 349_M 2.092 1.00
71_Y 79_A 2.087 1.00
326_G 355_I 2.005 1.00
107_P 111_M 1.99 1.00
351_Q 355_I 1.975 1.00
294_S 302_E 1.968 1.00
176_L 179_A 1.966 1.00
40_A 126_L 1.96 1.00
363_G 419_C 1.957 1.00
82_V 222_H 1.94 1.00
133_I 187_L 1.934 1.00
330_G 334_V 1.907 1.00
307_M 370_L 1.874 1.00
103_M 132_K 1.868 1.00
163_A 203_Y 1.854 1.00
163_A 204_Q 1.829 1.00
158_S 162_F 1.827 1.00
170_S 199_L 1.826 1.00
352_R 408_A 1.821 1.00
381_K 385_K 1.813 1.00
409_G 413_S 1.809 1.00
102_G 135_F 1.806 1.00
287_L 310_G 1.797 1.00
136_P 139_L 1.792 1.00
405_L 412_L 1.79 1.00
106_A 132_K 1.752 1.00
356_A 412_L 1.739 1.00
379_D 428_Q 1.735 1.00
330_G 351_Q 1.728 1.00
133_I 190_A 1.728 1.00
140_L 201_L 1.726 1.00
308_D 312_R 1.716 1.00
295_F 303_Y 1.704 1.00
84_V 150_I 1.704 1.00
143_L 201_L 1.701 1.00
295_F 377_R 1.696 1.00
167_L 200_Q 1.692 1.00
307_M 373_G 1.662 1.00
222_H 225_G 1.661 1.00
345_F 348_L 1.647 1.00
106_A 128_S 1.642 1.00
70_E 74_K 1.637 1.00
300_H 304_N 1.616 1.00
390_T 421_N 1.613 1.00
33_G 37_A 1.601 1.00
137_Y 194_T 1.594 1.00
68_L 153_T 1.575 1.00
155_N 293_K 1.57 1.00
28_V 189_L 1.564 1.00
291_L 303_Y 1.563 1.00
344_A 348_L 1.56 1.00
176_L 180_P 1.553 1.00
202_V 206_P 1.552 1.00
120_T 259_S 1.55 1.00
91_L 146_L 1.545 1.00
34_A 343_T 1.542 1.00
322_A 326_G 1.537 1.00
184_P 191_W 1.524 1.00
294_S 299_N 1.52 1.00
238_L 384_V 1.52 1.00
304_N 308_D 1.517 1.00
174_F 192_A 1.51 1.00
301_D 305_R 1.509 1.00
305_R 309_W 1.503 1.00
27_I 176_L 1.498 1.00
72_K 80_T 1.491 1.00
126_L 129_Q 1.486 1.00
125_A 129_Q 1.47 1.00
124_F 128_S 1.47 1.00
177_F 182_F 1.464 1.00
70_E 378_Q 1.449 1.00
253_F 402_I 1.449 1.00
393_L 420_L 1.445 1.00
187_L 191_W 1.445 1.00
79_A 82_V 1.442 1.00
386_I 424_L 1.442 1.00
133_I 191_W 1.433 1.00
385_K 389_V 1.43 1.00
353_A 407_H 1.429 1.00
443_A 447_R 1.424 1.00
324_A 485_V 1.42 1.00
122_D 125_A 1.411 1.00
175_A 179_A 1.406 0.99
71_Y 76_G 1.406 0.99
423_S 427_W 1.406 0.99
356_A 408_A 1.405 0.99
253_F 398_N 1.401 0.99
111_M 124_F 1.391 0.99
99_T 139_L 1.384 0.99
397_M 413_S 1.378 0.99
133_I 194_T 1.367 0.99
305_R 308_D 1.367 0.99
254_A 410_L 1.364 0.99
293_K 297_S 1.363 0.99
147_V 204_Q 1.362 0.99
20_L 24_R 1.361 0.99
250_N 265_M 1.36 0.99
250_N 398_N 1.358 0.99
90_L 226_A 1.354 0.99
162_F 166_L 1.35 0.99
402_I 410_L 1.339 0.99
177_F 181_Y 1.337 0.99
318_A 365_I 1.337 0.99
458_V 491_A 1.335 0.99
455_M 488_G 1.322 0.99
27_I 179_A 1.319 0.99
178_A 184_P 1.317 0.99
379_D 432_Q 1.301 0.99
413_S 417_A 1.299 0.99
170_S 174_F 1.295 0.99
324_A 455_M 1.294 0.99
169_I 173_G 1.287 0.99
173_G 177_F 1.28 0.99
427_W 431_K 1.276 0.99
44_A 131_L 1.276 0.99
331_P 334_V 1.275 0.99
185_P 188_A 1.269 0.99
67_I 232_Q 1.269 0.99
363_G 422_A 1.266 0.99
290_S 306_L 1.265 0.99
314_C 369_V 1.263 0.99
314_C 365_I 1.258 0.99
324_A 328_L 1.251 0.99
312_R 316_L 1.239 0.98
108_W 111_M 1.236 0.98
14_T 18_R 1.232 0.98
258_A 261_S 1.225 0.98
234_G 238_L 1.224 0.98
451_A 495_A 1.222 0.98
326_G 358_S 1.221 0.98
356_A 415_G 1.216 0.98
302_E 305_R 1.215 0.98
41_F 186_V 1.214 0.98
396_L 400_A 1.212 0.98
268_A 353_A 1.211 0.98
393_L 397_M 1.209 0.98
397_M 416_L 1.207 0.98
71_Y 74_K 1.205 0.98
401_F 405_L 1.204 0.98
175_A 189_L 1.201 0.98
110_I 127_T 1.198 0.98
110_I 124_F 1.197 0.98
24_R 193_V 1.183 0.98
25_D 270_R 1.182 0.98
108_W 112_V 1.176 0.98
121_A 125_A 1.174 0.98
73_S 297_S 1.174 0.98
242_V 388_I 1.174 0.98
254_A 407_H 1.173 0.98
105_A 109_V 1.172 0.98
64_F 68_L 1.17 0.97
206_P 210_K 1.168 0.97
348_L 352_R 1.168 0.97
93_L 97_V 1.167 0.97
349_M 352_R 1.167 0.97
323_V 456_S 1.163 0.97
392_I 396_L 1.163 0.97
67_I 83_F 1.159 0.97
316_L 496_L 1.154 0.97
107_P 132_K 1.15 0.97
405_L 409_G 1.148 0.97
107_P 125_A 1.142 0.97
86_Y 226_A 1.14 0.97
303_Y 377_R 1.136 0.97
254_A 265_M 1.134 0.97
111_M 121_A 1.132 0.97
250_N 414_I 1.13 0.97
316_L 320_P 1.125 0.97
235_P 384_V 1.12 0.96
261_S 407_H 1.118 0.96
11_S 282_A 1.117 0.96
372_P 376_S 1.114 0.96
100_V 104_L 1.113 0.96
159_I 207_H 1.112 0.96
401_F 413_S 1.112 0.96
347_A 350_T 1.108 0.96
103_M 107_P 1.104 0.96
398_N 402_I 1.104 0.96
331_P 335_S 1.103 0.96
77_E 81_R 1.101 0.96
70_E 228_R 1.101 0.96
386_I 428_Q 1.1 0.96
322_A 362_I 1.092 0.96
35_G 122_D 1.091 0.96
348_L 351_Q 1.087 0.96
459_L 488_G 1.087 0.96
99_T 103_M 1.086 0.96
16_F 165_T 1.083 0.96
386_I 425_L 1.077 0.95
328_L 332_L 1.077 0.95
37_A 126_L 1.077 0.95
338_Q 347_A 1.077 0.95
257_L 261_S 1.075 0.95
39_D 263_S 1.072 0.95
228_R 378_Q 1.071 0.95
31_I 180_P 1.07 0.95
82_V 86_Y 1.067 0.95
260_G 345_F 1.067 0.95
489_I 493_F 1.066 0.95
394_T 417_A 1.061 0.95
66_P 70_E 1.061 0.95
187_L 190_A 1.06 0.95
70_E 295_F 1.06 0.95
8_A 12_S 1.06 0.95
136_P 194_T 1.06 0.95
427_W 430_R 1.06 0.95
176_L 181_Y 1.059 0.95
495_A 499_L 1.059 0.95
401_F 409_G 1.056 0.95
455_M 459_L 1.053 0.95
367_V 418_A 1.052 0.95
253_F 399_L 1.052 0.95
224_A 227_M 1.05 0.94
179_A 189_L 1.046 0.94
456_S 460_L 1.046 0.94
144_A 200_Q 1.046 0.94
454_V 495_A 1.043 0.94
163_A 207_H 1.043 0.94
170_S 196_G 1.038 0.94
71_Y 83_F 1.037 0.94
198_V 202_V 1.031 0.94
24_R 171_M 1.031 0.94
69_A 153_T 1.029 0.94
166_L 203_Y 1.028 0.94
83_F 225_G 1.026 0.94
264_W 350_T 1.025 0.93
374_F 428_Q 1.023 0.93
333_T 347_A 1.023 0.93
99_T 136_P 1.023 0.93
78_D 82_V 1.022 0.93
343_T 346_D 1.021 0.93
320_P 323_V 1.021 0.93
27_I 175_A 1.018 0.93
333_T 351_Q 1.017 0.93
412_L 416_L 1.017 0.93
79_A 225_G 1.016 0.93
87_V 229_V 1.013 0.93
271_L 332_L 1.01 0.93
389_V 392_I 1.01 0.93
89_G 93_L 1.009 0.93
394_T 398_N 1.007 0.93
73_S 296_A 1.005 0.93
64_F 83_F 1.005 0.93
235_P 380_I 1.003 0.93
137_Y 197_G 1.002 0.92
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
4lz6A10.83561000.323Contact Map0.588
4humA10.84931000.335Contact Map0.307
3w4tA10.84741000.338Contact Map0.733
3mktA20.84541000.34Contact Map0.38
2cfqA10.75153.60.97Contact Map0.191
1nkzB30.08021.90.974Contact Map0.469
2rddB10.06461.70.974Contact Map0.45
3rfuA40.11741.70.974Contact Map0.653
4jc9S140.09591.50.975Contact Map0.851
1lghB40.08811.40.976Contact Map0.494
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
Page generated in 0.0383 seconds.