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OPENSEQ.org
ENVZ - Osmolarity sensor protein EnvZ
UniProt: P0AEJ4 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG10269
Length: 450 (434)
Sequences: 2388
Seq/Len: 5.50
ENVZ
Paralog alert: 0.70 [within 20: 0.03] - ratio of genomes with paralogs
Cluster includes: ATOS BAES BASS CPXA CREC CUSS DCUS DPIB ENVZ GLRK NTRB PHOQ PHOR QSEC RSTB YEDV ZRAS
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
372_E 431_S 4.812 1.00
383_R 424_T 4.705 1.00
387_F 420_L 4.636 1.00
418_G 436_L 4.111 1.00
422_L 432_I 3.52 1.00
409_V 420_L 3.365 1.00
413_V 434_A 3.204 1.00
386_L 422_L 3.028 1.00
340_A 434_A 2.949 1.00
410_Q 414_D 2.924 1.00
340_A 412_I 2.914 1.00
370_Q 433_R 2.79 1.00
422_L 430_L 2.779 1.00
340_A 413_V 2.779 1.00
387_F 410_Q 2.77 1.00
379_A 382_Q 2.673 1.00
413_V 418_G 2.64 1.00
253_R 275_E 2.636 1.00
378_I 386_L 2.626 1.00
423_G 433_R 2.571 1.00
345_V 358_V 2.564 1.00
359_S 370_Q 2.522 1.00
412_I 416_H 2.514 1.00
382_Q 385_H 2.447 1.00
373_D 377_G 2.4 1.00
382_Q 391_V 2.351 1.00
385_H 388_Q 2.333 1.00
352_G 374_D 2.315 1.00
332_M 337_I 2.289 1.00
359_S 372_E 2.244 1.00
252_I 270_I 2.227 1.00
372_E 425_S 2.089 1.00
332_M 416_H 2.048 1.00
300_D 303_A 2.041 1.00
366_R 435_W 2.033 1.00
370_Q 431_S 2.027 1.00
251_R 273_D 2.004 1.00
419_M 435_W 1.929 1.00
418_G 437_P 1.92 1.00
386_L 432_I 1.905 1.00
387_F 432_I 1.891 1.00
368_W 435_W 1.884 1.00
344_M 432_I 1.877 1.00
414_D 419_M 1.87 1.00
305_L 341_V 1.852 1.00
413_V 420_L 1.835 1.00
357_K 374_D 1.819 1.00
425_S 428_G 1.814 1.00
373_D 429_G 1.805 1.00
305_L 360_S 1.801 1.00
249_L 274_I 1.794 1.00
298_M 331_K 1.754 1.00
308_V 345_V 1.754 1.00
420_L 432_I 1.725 1.00
278_N 282_E 1.707 1.00
424_T 430_L 1.684 1.00
367_A 438_V 1.684 1.00
361_G 368_W 1.646 0.99
423_G 431_S 1.624 0.99
362_T 438_V 1.615 0.99
299_A 337_I 1.612 0.99
386_L 391_V 1.603 0.99
421_E 433_R 1.601 0.99
385_H 391_V 1.557 0.99
256_T 270_I 1.546 0.99
322_A 357_K 1.541 0.99
394_D 397_R 1.537 0.99
361_G 370_Q 1.534 0.99
355_W 374_D 1.521 0.99
351_Y 392_R 1.518 0.99
236_L 240_G 1.517 0.99
251_R 254_L 1.501 0.99
194_L 222_H 1.497 0.99
404_L 408_I 1.494 0.99
363_E 435_W 1.483 0.99
301_L 367_A 1.469 0.99
357_K 372_E 1.447 0.98
368_W 433_R 1.442 0.98
297_E 334_P 1.437 0.98
388_Q 410_Q 1.434 0.98
256_T 274_I 1.428 0.98
337_I 436_L 1.427 0.98
299_A 332_M 1.426 0.98
345_V 371_V 1.424 0.98
237_L 241_V 1.422 0.98
374_D 427_R 1.421 0.98
380_P 383_R 1.416 0.98
244_D 339_R 1.403 0.98
237_L 244_D 1.398 0.98
237_L 284_F 1.388 0.98
363_E 368_W 1.385 0.98
383_R 423_G 1.382 0.98
186_L 213_V 1.378 0.98
307_E 338_K 1.375 0.98
193_A 223_M 1.371 0.97
294_M 333_H 1.364 0.97
387_F 391_V 1.355 0.97
220_F 223_M 1.354 0.97
207_E 218_R 1.338 0.97
10_S 14_R 1.335 0.97
344_M 434_A 1.327 0.97
11_S 14_R 1.32 0.97
309_I 358_V 1.317 0.97
425_S 431_S 1.315 0.97
22_L 26_S 1.306 0.96
380_P 424_T 1.304 0.96
250_T 254_L 1.304 0.96
413_V 436_L 1.304 0.96
283_Q 402_T 1.299 0.96
346_V 350_R 1.298 0.96
205_L 221_N 1.296 0.96
12_F 180_R 1.293 0.96
372_E 426_E 1.293 0.96
305_L 358_V 1.292 0.96
343_N 409_V 1.287 0.96
340_A 416_H 1.286 0.96
305_L 369_F 1.286 0.96
383_R 422_L 1.283 0.96
366_R 419_M 1.282 0.96
354_G 374_D 1.281 0.96
195_Q 200_I 1.275 0.96
341_V 369_F 1.271 0.96
244_D 251_R 1.269 0.95
367_A 436_L 1.268 0.95
307_E 334_P 1.262 0.95
330_V 367_A 1.255 0.95
378_I 430_L 1.244 0.95
377_G 429_G 1.243 0.95
328_I 367_A 1.234 0.95
387_F 422_L 1.227 0.94
383_R 387_F 1.219 0.94
320_E 357_K 1.209 0.94
244_D 284_F 1.205 0.94
240_G 284_F 1.205 0.94
392_R 409_V 1.187 0.93
315_Y 346_V 1.186 0.93
236_L 411_R 1.186 0.93
181_I 188_D 1.166 0.92
12_F 16_L 1.164 0.92
204_P 225_A 1.151 0.91
273_D 276_E 1.146 0.91
149_L 152_I 1.143 0.91
384_K 387_F 1.143 0.91
363_E 366_R 1.139 0.91
241_V 284_F 1.137 0.91
304_V 338_K 1.13 0.90
370_Q 423_G 1.129 0.90
12_F 15_T 1.129 0.90
372_E 427_R 1.103 0.89
341_V 345_V 1.094 0.88
420_L 434_A 1.089 0.88
300_D 327_S 1.084 0.88
369_F 376_P 1.081 0.88
301_L 369_F 1.073 0.87
253_R 271_N 1.063 0.86
234_R 408_I 1.062 0.86
383_R 386_L 1.056 0.86
386_L 430_L 1.051 0.86
175_A 179_I 1.045 0.85
169_L 173_G 1.044 0.85
251_R 389_P 1.043 0.85
163_Y 167_I 1.042 0.85
41_P 45_Q 1.041 0.85
205_L 217_T 1.038 0.85
245_L 277_C 1.036 0.84
348_A 356_I 1.034 0.84
240_G 389_P 1.032 0.84
376_P 430_L 1.031 0.84
276_E 338_K 1.023 0.83
379_A 393_G 1.023 0.83
14_R 181_I 1.023 0.83
277_C 285_I 1.022 0.83
283_Q 411_R 1.018 0.83
330_V 436_L 1.014 0.83
392_R 401_G 1.013 0.83
179_I 182_Q 1.013 0.83
249_L 277_C 1.012 0.82
305_L 308_V 1.007 0.82
240_G 255_A 1.002 0.82
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
4i5sA20.67111000.483Contact Map0.778
4ew8A20.55781000.58Contact Map0.733
2btzA10.67331000.584Contact Map0.58
3jz3A20.461000.594Contact Map0.66
2q8gA10.69561000.6Contact Map0.562
2c2aA10.52221000.603Contact Map0.725
4e01A10.72891000.604Contact Map0.616
3d36A20.50221000.606Contact Map0.709
1y8oA10.721000.61Contact Map0.593
2e0aA20.681000.613Contact Map0.633
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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