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OPENSEQ.org

KEFG - Glutathione-regulated potassium-efflux system ancillary protein KefG
UniProt: P0A756 Sequence
Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG12902
Length: 184 (174)
Sequences: 2588
Seq/Len: 14.87

KEFG
Paralog alert: 0.61 [within 20: 0.03] - ratio of genomes with paralogs
Cluster includes: AZOR KEFF KEFG MDAB NQOR YCAK
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
39_H 58_L 3.354 1.00
37_T 61_E 3.109 1.00
7_K 35_N 2.913 1.00
40_D 43_A 2.737 1.00
106_W 140_A 2.69 1.00
37_T 58_L 2.565 1.00
57_A 61_E 2.488 1.00
82_E 86_R 2.381 1.00
81_K 85_D 2.128 1.00
37_T 62_H 2.125 1.00
106_W 145_M 1.927 1.00
65_I 145_M 1.851 1.00
64_V 105_Y 1.835 1.00
76_C 135_P 1.779 1.00
7_K 61_E 1.765 1.00
161_A 165_A 1.762 1.00
13_A 79_L 1.707 1.00
84_L 139_A 1.65 1.00
8_V 34_S 1.642 1.00
108_S 150_P 1.632 1.00
7_K 37_T 1.631 1.00
166_S 170_A 1.621 1.00
53_P 57_A 1.603 1.00
56_Q 92_F 1.556 1.00
8_V 64_V 1.544 1.00
57_A 60_R 1.538 1.00
109_V 151_I 1.496 1.00
26_L 30_A 1.489 1.00
64_V 107_R 1.486 1.00
29_P 172_G 1.475 1.00
105_Y 146_H 1.472 1.00
60_R 92_F 1.466 1.00
112_T 154_Y 1.457 1.00
47_D 78_A 1.445 1.00
11_L 39_H 1.445 1.00
74_Y 142_M 1.442 1.00
15_P 72_Y 1.433 1.00
169_R 172_G 1.429 1.00
23_N 68_Q 1.414 1.00
110_I 152_I 1.394 0.99
160_S 163_E 1.391 0.99
56_Q 93_A 1.334 0.99
12_Y 27_L 1.331 0.99
167_H 170_A 1.321 0.99
12_Y 38_V 1.319 0.99
65_I 106_W 1.319 0.99
23_N 111_T 1.316 0.99
88_L 143_C 1.302 0.99
44_H 58_L 1.285 0.99
76_C 136_F 1.279 0.99
13_A 42_Y 1.265 0.99
52_I 56_Q 1.264 0.99
84_L 88_L 1.262 0.99
101_L 145_M 1.261 0.99
103_G 146_H 1.25 0.99
78_A 82_E 1.249 0.99
102_A 144_R 1.236 0.98
163_E 166_S 1.223 0.98
24_R 28_K 1.222 0.98
10_L 38_V 1.222 0.98
165_A 169_R 1.22 0.98
69_H 108_S 1.206 0.98
28_K 32_Q 1.205 0.98
15_P 79_L 1.184 0.98
164_L 168_A 1.165 0.97
172_G 176_A 1.159 0.97
116_E 154_Y 1.138 0.97
29_P 32_Q 1.138 0.97
13_A 80_L 1.114 0.96
55_E 83_W 1.112 0.96
62_H 65_I 1.105 0.96
51_D 54_R 1.101 0.96
128_P 131_D 1.098 0.96
105_Y 149_S 1.098 0.96
72_Y 77_P 1.091 0.96
44_H 54_R 1.09 0.96
39_H 44_H 1.082 0.96
87_V 143_C 1.067 0.95
26_L 109_V 1.048 0.94
11_L 67_F 1.04 0.94
69_H 80_L 1.04 0.94
99_N 144_R 1.034 0.94
59_L 65_I 1.031 0.94
27_L 38_V 1.03 0.94
161_A 164_L 1.028 0.94
30_A 171_Y 1.026 0.94
53_P 89_S 1.025 0.93
67_F 140_A 1.008 0.93
60_R 100_Q 1.008 0.93
27_L 30_A 1.008 0.93
30_A 109_V 1.007 0.93
22_A 26_L 1.004 0.93
169_R 173_D 1.004 0.93
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
1d4aA40.96741000.359Contact Map0.731
4gi5A20.98371000.36Contact Map0.768
3l9wA20.98911000.363Contact Map0.73
2amjA40.94021000.396Contact Map0.746
3f2vA10.97831000.404Contact Map0.687
3rpeA20.94571000.415Contact Map0.742
4fglA40.98371000.417Contact Map0.761
3ha2A20.93481000.421Contact Map0.678
3r6wA20.94021000.444Contact Map0.693
3lcmA40.92931000.452Contact Map0.804

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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