OPENSEQ.org - KEFF - ECOLI GREMLIN

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KEFF - Glutathione-regulated potassium-efflux system ancillary protein KefF

UniProt:	P0A754	Sequence MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNIDIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVFSHGWAYGHGGTALHGKHLLWAVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQARHYKQRLLEWQEAHHG	Download Alignment We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene:	EG11568
Length:	176 (167)
Sequences:	1632
Seq/Len:	9.77

KEFF
Paralog alert: 0.60 [within 20: 0.03] - ratio of genomes with paralogs
Cluster includes: AZOR KEFF KEFG MDAB NQOR YCAK

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Scaled Score	Prob
31_E	55_R	4.172	1.00
34_S	37_Q	2.924	1.00
33_R	52_A	2.783	1.00
76_L	80_K	2.68	1.00
31_E	52_A	2.659	1.00
1_M	29_G	2.646	1.00
100_L	132_A	2.242	1.00
7_A	73_L	2.136	1.00
75_K	79_D	2.125	1.00
31_E	56_A	2.037	1.00
58_L	99_H	2.031	1.00
99_H	138_N	2.017	1.00
50_Q	86_W	2.008	1.00
33_R	38_L	1.997	1.00
59_I	137_L	1.973	1.00
51_E	55_R	1.888	1.00
47_A	51_E	1.834	1.00
6_Y	21_L	1.797	1.00
65_M	104_V	1.796	1.00
153_D	157_E	1.78	1.00
1_M	55_R	1.725	1.00
54_S	86_W	1.717	1.00
49_E	77_W	1.656	1.00
152_D	155_T	1.626	1.00
7_A	36_Y	1.617	1.00
17_N	105_T	1.601	1.00
70_I	128_L	1.597	1.00
17_N	62_Q	1.566	1.00
72_P	76_L	1.527	1.00
50_Q	87_A	1.526	1.00
161_R	164_K	1.522	1.00
21_L	32_I	1.509	1.00
103_A	163_Y	1.499	1.00
38_L	52_A	1.483	1.00
46_I	50_Q	1.474	1.00
38_L	48_A	1.473	1.00
2_I	58_L	1.469	1.00
39_Y	44_I	1.45	1.00
48_A	52_A	1.444	1.00
103_A	143_F	1.443	1.00
96_H	136_G	1.425	1.00
110_E	146_H	1.405	0.99
16_A	105_T	1.384	0.99
159_Q	162_H	1.363	0.99
132_A	137_L	1.36	0.99
2_I	28_E	1.341	0.99
33_R	49_E	1.327	0.99
51_E	54_S	1.293	0.99
63_H	102_W	1.26	0.99
161_R	165_Q	1.247	0.98
23_Q	164_K	1.22	0.98
70_I	127_P	1.19	0.98
158_G	162_H	1.189	0.98
42_F	76_L	1.175	0.97
106_T	146_H	1.172	0.97
110_E	114_E	1.171	0.97
145_M	159_Q	1.165	0.97
5_I	33_R	1.159	0.97
54_S	94_A	1.152	0.97
155_T	158_G	1.152	0.97
62_Q	163_Y	1.147	0.97
3_L	52_A	1.144	0.97
121_F	126_Q	1.133	0.97
74_L	78_I	1.125	0.96
68_Y	134_Y	1.12	0.96
9_P	66_Q	1.11	0.96
104_V	144_A	1.102	0.96
62_Q	103_A	1.099	0.96
20_M	105_T	1.095	0.96
67_W	88_Y	1.093	0.96
53_L	82_F	1.086	0.95
45_D	48_A	1.083	0.95
47_A	83_S	1.082	0.95
100_L	137_L	1.073	0.95
6_Y	11_P	1.069	0.95
156_L	160_A	1.048	0.94
103_A	145_M	1.045	0.94
78_I	82_F	1.043	0.94
53_L	59_I	1.043	0.94
52_A	55_R	1.042	0.94
93_T	96_H	1.028	0.93
95_L	137_L	1.016	0.93
57_D	98_K	1.006	0.92
38_L	49_E	1.002	0.92

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling

HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:

Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	P(%)	HHΔ		Acc
1d4aA	4	0.9886	100	0.392	Contact Map	0.758
3l9wA	2	1	100	0.399	Contact Map	0.797
4gi5A	2	0.9886	100	0.404	Contact Map	0.81
2amjA	4	0.9318	100	0.417	Contact Map	0.773
3ha2A	2	0.9545	100	0.438	Contact Map	0.724
3f2vA	1	1	100	0.441	Contact Map	0.709
4fglA	4	0.9886	100	0.456	Contact Map	0.771
3rpeA	2	0.9432	100	0.464	Contact Map	0.793
3lcmA	4	0.9602	100	0.477	Contact Map	0.839
3r6wA	2	0.9659	99.9	0.534	Contact Map	0.616

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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