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OPENSEQ.org
SFMD - Outer membrane usher protein SfmD
UniProt: P77468 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG13883
Length: 867 (796)
Sequences: 1279
Seq/Len: 1.61
SFMD
Paralog alert: 0.75 [within 20: 0.05] - ratio of genomes with paralogs
Cluster includes: ELFC FIMD HTRE SFMD YAGX YBGQ YEHB YFCU YHCD YQIG YRAJ
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
103_C 130_C 4.386 1.00
289_V 301_I 4.108 1.00
704_V 735_A 3.862 1.00
297_A 338_E 3.738 1.00
666_G 681_Q 3.693 1.00
253_D 267_R 3.126 1.00
696_S 731_V 3.065 1.00
783_A 822_A 2.995 1.00
800_V 828_L 2.901 1.00
694_T 725_D 2.899 1.00
362_F 390_G 2.843 1.00
266_F 383_F 2.821 1.00
668_G 679_N 2.789 1.00
298_Q 337_D 2.71 1.00
335_T 343_Q 2.675 1.00
686_V 695_L 2.637 1.00
294_R 342_N 2.621 1.00
826_G 844_Y 2.577 1.00
707_K 774_N 2.556 1.00
362_F 388_A 2.549 1.00
688_G 693_I 2.487 1.00
337_D 343_Q 2.462 1.00
528_G 553_Y 2.414 1.00
689_H 694_T 2.408 1.00
800_V 817_V 2.401 1.00
798_S 830_V 2.373 1.00
294_R 344_Q 2.368 1.00
336_I 344_Q 2.362 1.00
333_D 345_N 2.326 1.00
302_R 307_I 2.302 1.00
209_P 240_R 2.29 0.99
434_S 480_V 2.289 0.99
639_H 655_T 2.275 0.99
301_I 320_I 2.241 0.99
186_F 199_F 2.209 0.99
216_G 231_W 2.198 0.99
787_V 842_A 2.19 0.99
705_L 729_Y 2.182 0.99
179_A 770_L 2.144 0.99
641_S 653_S 2.089 0.99
531_Y 552_G 2.076 0.99
86_F 102_P 2.045 0.99
555_S 562_Y 2.042 0.99
70_V 86_F 2.025 0.98
73_W 150_R 2.019 0.98
212_L 238_V 2.012 0.98
399_Y 428_D 2.011 0.98
70_V 144_F 1.997 0.98
428_D 448_R 1.996 0.98
132_N 135_K 1.987 0.98
177_I 705_L 1.984 0.98
610_F 622_W 1.974 0.98
685_G 696_S 1.959 0.98
302_R 335_T 1.89 0.97
703_N 770_L 1.877 0.97
691_N 707_K 1.867 0.97
707_K 772_R 1.867 0.97
261_F 401_G 1.835 0.97
300_T 307_I 1.819 0.96
685_G 699_L 1.802 0.96
665_L 682_L 1.795 0.96
689_H 729_Y 1.775 0.96
204_G 213_R 1.764 0.96
663_G 682_L 1.744 0.95
366_L 386_G 1.734 0.95
212_L 236_T 1.725 0.95
565_S 582_G 1.722 0.95
741_N 765_P 1.72 0.95
828_L 842_A 1.718 0.95
167_Y 170_P 1.715 0.95
115_N 118_A 1.702 0.94
446_S 470_S 1.699 0.94
446_S 468_R 1.696 0.94
205_I 212_L 1.688 0.94
781_V 824_L 1.682 0.94
242_I 245_L 1.668 0.93
154_T 314_S 1.653 0.93
686_V 693_I 1.651 0.93
72_L 84_I 1.638 0.93
701_D 767_Q 1.626 0.92
53_D 57_F 1.621 0.92
239_Q 248_E 1.62 0.92
270_R 365_D 1.62 0.92
143_N 152_N 1.619 0.92
250_V 272_Y 1.608 0.92
254_G 367_T 1.605 0.92
708_A 777_T 1.603 0.92
685_G 731_V 1.593 0.91
130_C 356_L 1.587 0.91
787_V 817_V 1.586 0.91
699_L 703_N 1.585 0.91
708_A 711_A 1.583 0.91
300_T 337_D 1.581 0.91
609_S 625_G 1.564 0.90
142_I 153_I 1.563 0.90
800_V 830_V 1.56 0.90
276_N 767_Q 1.559 0.90
103_C 356_L 1.556 0.90
254_G 266_F 1.556 0.90
753_S 781_V 1.555 0.90
348_I 356_L 1.55 0.90
690_E 772_R 1.549 0.90
211_R 766_T 1.548 0.90
446_S 540_W 1.546 0.89
289_V 332_L 1.528 0.89
165_H 324_H 1.524 0.89
460_T 469_Y 1.506 0.88
275_D 363_K 1.503 0.88
67_V 83_D 1.5 0.88
336_I 346_Y 1.486 0.87
286_A 358_R 1.482 0.87
290_R 319_E 1.478 0.86
685_G 703_N 1.475 0.86
275_D 361_R 1.473 0.86
181_L 703_N 1.468 0.86
554_T 563_S 1.467 0.86
293_A 313_V 1.466 0.86
787_V 830_V 1.461 0.86
515_R 537_Q 1.458 0.85
69_R 83_D 1.454 0.85
333_D 347_T 1.441 0.85
182_L 203_S 1.438 0.84
765_P 771_V 1.438 0.84
794_V 830_V 1.42 0.83
178_P 205_I 1.418 0.83
160_L 269_V 1.414 0.83
175_E 243_I 1.413 0.83
529_S 817_V 1.409 0.83
711_A 777_T 1.408 0.83
105_N 108_L 1.407 0.83
431_H 445_D 1.404 0.82
704_V 743_I 1.403 0.82
354_P 383_F 1.39 0.82
266_F 367_T 1.384 0.81
280_D 739_R 1.368 0.80
795_P 798_S 1.367 0.80
214_N 233_N 1.366 0.80
206_N 213_R 1.365 0.80
73_W 78_F 1.363 0.80
396_F 417_R 1.36 0.80
410_A 430_T 1.36 0.80
740_Y 762_S 1.357 0.79
145_D 152_N 1.356 0.79
789_Q 794_V 1.355 0.79
714_V 724_T 1.355 0.79
103_C 348_I 1.353 0.79
660_A 663_G 1.349 0.79
241_A 766_T 1.342 0.78
568_W 579_R 1.34 0.78
840_C 862_C 1.338 0.78
687_V 705_L 1.331 0.77
296_A 338_E 1.326 0.77
691_N 772_R 1.325 0.77
392_L 784_L 1.318 0.77
241_A 248_E 1.317 0.76
532_I 551_V 1.316 0.76
130_C 348_I 1.312 0.76
359_E 394_Q 1.311 0.76
725_D 731_V 1.31 0.76
177_I 770_L 1.303 0.75
716_I 732_M 1.302 0.75
399_Y 414_G 1.293 0.75
458_F 522_Q 1.283 0.74
688_G 705_L 1.279 0.73
266_F 270_R 1.277 0.73
414_G 423_G 1.274 0.73
188_G 680_W 1.265 0.72
178_P 690_E 1.263 0.72
186_F 197_Y 1.262 0.72
391_G 423_G 1.262 0.72
178_P 770_L 1.258 0.72
687_V 703_N 1.257 0.72
262_D 476_T 1.256 0.71
302_R 333_D 1.255 0.71
708_A 714_V 1.255 0.71
730_A 733_L 1.254 0.71
687_V 770_L 1.25 0.71
689_H 692_G 1.245 0.71
615_N 618_G 1.244 0.70
108_L 112_I 1.243 0.70
789_Q 792_K 1.239 0.70
267_R 372_R 1.238 0.70
524_L 528_G 1.234 0.70
732_M 743_I 1.231 0.69
707_K 729_Y 1.23 0.69
74_R 160_L 1.227 0.69
370_D 382_F 1.226 0.69
780_G 821_G 1.225 0.69
65_A 651_T 1.225 0.69
287_P 391_G 1.225 0.69
660_A 665_L 1.222 0.68
614_R 619_Q 1.221 0.68
694_T 727_R 1.216 0.68
787_V 811_V 1.216 0.68
785_I 858_I 1.215 0.68
787_V 794_V 1.212 0.68
462_F 519_N 1.209 0.67
334_V 348_I 1.208 0.67
518_L 532_I 1.202 0.67
795_P 832_W 1.201 0.67
73_W 152_N 1.201 0.67
709_P 724_T 1.199 0.66
298_Q 310_Q 1.199 0.66
706_I 830_V 1.19 0.66
798_S 832_W 1.188 0.65
783_A 846_L 1.188 0.65
724_T 782_R 1.187 0.65
300_T 310_Q 1.187 0.65
71_D 81_S 1.184 0.65
250_V 254_G 1.183 0.65
351_S 356_L 1.182 0.65
827_E 841_I 1.182 0.65
465_M 518_L 1.181 0.65
450_L 463_Q 1.177 0.64
557_W 562_Y 1.176 0.64
709_P 727_R 1.172 0.64
241_A 348_I 1.171 0.64
71_D 74_R 1.171 0.64
519_N 584_N 1.169 0.63
513_K 540_W 1.168 0.63
697_Q 721_G 1.165 0.63
109_L 114_L 1.163 0.63
403_Q 477_L 1.163 0.63
756_V 759_N 1.161 0.63
360_G 423_G 1.158 0.62
137_V 140_A 1.156 0.62
445_D 471_T 1.155 0.62
180_L 205_I 1.153 0.62
359_E 393_P 1.151 0.62
177_I 690_E 1.148 0.61
184_Y 684_G 1.148 0.61
297_A 340_D 1.147 0.61
144_F 149_M 1.146 0.61
391_G 397_T 1.144 0.61
336_I 348_I 1.144 0.61
689_H 705_L 1.143 0.61
389_L 782_R 1.142 0.61
842_A 860_A 1.14 0.61
536_H 547_T 1.135 0.60
130_C 477_L 1.123 0.59
247_S 364_F 1.122 0.59
202_L 215_N 1.122 0.59
240_R 249_L 1.121 0.59
514_D 538_K 1.118 0.58
633_G 716_I 1.113 0.58
782_R 820_S 1.113 0.58
472_Q 743_I 1.11 0.58
446_S 467_Y 1.107 0.57
399_Y 448_R 1.105 0.57
119_F 159_A 1.104 0.57
699_L 770_L 1.103 0.57
241_A 738_Y 1.101 0.57
458_F 534_G 1.101 0.57
262_D 446_S 1.101 0.57
392_L 398_A 1.1 0.57
694_T 705_L 1.099 0.56
615_N 619_Q 1.099 0.56
242_I 271_L 1.098 0.56
214_N 236_T 1.098 0.56
287_P 414_G 1.097 0.56
538_K 545_S 1.096 0.56
173_W 764_V 1.095 0.56
237_W 768_G 1.094 0.56
110_E 161_L 1.093 0.56
173_W 796_F 1.093 0.56
177_I 687_V 1.091 0.56
524_L 553_Y 1.09 0.55
335_T 345_N 1.087 0.55
162_S 321_T 1.087 0.55
103_C 702_T 1.085 0.55
428_D 446_S 1.084 0.55
630_L 638_Y 1.082 0.55
176_G 303_Q 1.082 0.55
274_S 767_Q 1.082 0.55
181_L 770_L 1.081 0.55
318_F 338_E 1.08 0.54
241_A 344_Q 1.078 0.54
706_I 716_I 1.077 0.54
185_N 716_I 1.077 0.54
247_S 273_S 1.077 0.54
67_V 85_V 1.075 0.54
251_M 269_V 1.075 0.54
172_E 740_Y 1.072 0.54
315_P 618_G 1.07 0.53
224_N 706_I 1.07 0.53
463_Q 517_Q 1.069 0.53
536_H 545_S 1.069 0.53
295_T 410_A 1.069 0.53
63_Q 100_L 1.068 0.53
766_T 769_A 1.066 0.53
63_Q 146_F 1.064 0.53
233_N 253_D 1.064 0.53
249_L 271_L 1.062 0.53
340_D 770_L 1.062 0.53
430_T 446_S 1.06 0.52
65_A 343_Q 1.052 0.51
785_I 819_L 1.05 0.51
429_V 530_L 1.049 0.51
458_F 465_M 1.044 0.51
320_I 733_L 1.043 0.51
371_F 481_A 1.041 0.50
562_Y 583_L 1.04 0.50
694_T 729_Y 1.039 0.50
523_S 615_N 1.039 0.50
419_L 529_S 1.036 0.50
159_A 317_A 1.034 0.50
530_L 609_S 1.033 0.49
466_G 479_D 1.032 0.49
157_Q 284_G 1.031 0.49
182_L 305_G 1.031 0.49
813_D 820_S 1.03 0.49
626_V 630_L 1.03 0.49
399_Y 796_F 1.029 0.49
567_S 580_I 1.028 0.49
563_S 584_N 1.027 0.49
149_M 357_Q 1.026 0.49
262_D 399_Y 1.025 0.49
72_L 491_D 1.025 0.49
103_C 477_L 1.022 0.48
283_Q 739_R 1.021 0.48
833_G 836_A 1.02 0.48
463_Q 467_Y 1.02 0.48
521_S 531_Y 1.019 0.48
181_L 687_V 1.017 0.48
180_L 688_G 1.013 0.47
211_R 769_A 1.013 0.47
689_H 725_D 1.013 0.47
259_D 363_K 1.012 0.47
467_Y 470_S 1.011 0.47
724_T 819_L 1.01 0.47
780_G 818_Y 1.01 0.47
612_A 620_N 1.01 0.47
434_S 563_S 1.01 0.47
273_S 278_Y 1.008 0.47
827_E 843_H 1.007 0.47
475_Y 696_S 1.007 0.47
147_A 803_N 1.005 0.47
264_V 403_Q 1 0.46
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
3rfzB20.93431000.127Contact Map0.704
3fipA20.54211000.488Contact Map0.707
3fcgA20.10381000.844Contact Map0.782
4b0mA10.15571000.858Contact Map0.852
3bwuD10.14421000.872Contact Map0.881
2xetA20.099299.70.91Contact Map0.889
3l48A50.100399.70.917Contact Map0.951
3e8vA10.085476.50.979Contact Map0.414
4kdwA10.1303630.981Contact Map0.491
3pe9A40.107327.80.985Contact Map0.709
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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