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OPENSEQ.org
K6PF2 - 6-phosphofructokinase isozyme 2
UniProt: P06999 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG10700
Length: 309 (301)
Sequences: 9592
Seq/Len: 31.87
K6PF2
Paralog alert: 0.85 [within 20: 0.18] - ratio of genomes with paralogs
Cluster includes: FRLD K1PF K6PF2 KDGK PSUK RBSK YDJH YEGV YEII YIHV
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
53_G 264_L 2.966 1.00
271_S 274_E 2.907 1.00
232_G 276_V 2.719 1.00
48_I 53_G 2.641 1.00
230_A 258_M 2.397 1.00
135_V 164_I 2.385 1.00
56_T 79_P 2.367 1.00
265_K 270_A 2.332 1.00
50_H 76_E 2.306 1.00
164_I 263_T 2.305 1.00
70_V 85_A 2.228 1.00
67_E 85_A 2.221 1.00
296_S 299_D 2.197 1.00
213_V 233_V 2.185 1.00
225_L 231_L 2.131 1.00
257_S 282_A 2.112 1.00
265_K 278_F 2.06 1.00
153_I 163_C 2.033 1.00
289_N 299_D 2.031 1.00
47_A 260_G 2.031 1.00
182_E 219_K 2.028 1.00
60_P 117_E 2.01 1.00
270_A 274_E 2.007 1.00
5_Y 131_G 1.994 1.00
97_V 100_S 1.978 1.00
71_S 75_D 1.94 1.00
188_Q 225_L 1.902 1.00
261_A 282_A 1.891 1.00
151_Q 154_S 1.877 1.00
156_A 161_I 1.837 1.00
297_H 301_Q 1.836 1.00
273_E 277_R 1.788 1.00
262_M 275_M 1.784 1.00
46_R 76_E 1.761 1.00
3_R 132_A 1.753 1.00
239_I 273_E 1.746 1.00
283_G 287_T 1.72 1.00
268_E 297_H 1.712 1.00
155_A 159_Q 1.706 1.00
63_G 67_E 1.693 1.00
6_T 45_A 1.686 1.00
230_A 280_V 1.668 1.00
162_R 182_E 1.656 1.00
185_K 259_V 1.64 1.00
72_L 75_D 1.633 1.00
250_S 291_G 1.624 1.00
225_L 240_Q 1.621 1.00
74_A 80_V 1.616 1.00
37_P 69_L 1.611 1.00
181_I 184_V 1.598 1.00
70_V 74_A 1.597 1.00
48_I 264_L 1.593 1.00
150_T 154_S 1.581 1.00
129_E 159_Q 1.581 1.00
157_Q 182_E 1.571 1.00
201_Q 204_D 1.569 1.00
222_V 262_M 1.547 1.00
133_I 263_T 1.546 1.00
254_A 287_T 1.543 1.00
211_E 214_N 1.541 1.00
151_Q 155_A 1.536 1.00
262_M 279_G 1.518 1.00
244_P 281_A 1.511 1.00
183_L 220_R 1.508 1.00
155_A 158_K 1.502 1.00
35_F 68_H 1.497 1.00
204_D 207_K 1.469 1.00
67_E 71_S 1.461 1.00
210_Q 214_N 1.46 1.00
246_V 288_L 1.455 1.00
241_V 276_V 1.448 1.00
70_V 83_V 1.445 1.00
166_D 224_S 1.445 1.00
209_A 233_V 1.438 1.00
133_I 162_R 1.429 1.00
162_R 219_K 1.425 1.00
63_G 86_K 1.423 1.00
231_L 240_Q 1.418 1.00
133_I 164_I 1.416 1.00
154_S 158_K 1.409 1.00
167_S 186_P 1.399 0.99
261_A 278_F 1.392 0.99
262_M 266_L 1.389 0.99
244_P 277_R 1.388 0.99
234_D 237_N 1.368 0.99
230_A 276_V 1.36 0.99
211_E 215_S 1.355 0.99
191_L 223_V 1.337 0.99
225_L 228_Q 1.334 0.99
220_R 234_D 1.33 0.99
11_P 59_F 1.327 0.99
49_A 78_V 1.326 0.99
220_R 262_M 1.317 0.99
232_G 239_I 1.313 0.99
48_I 55_A 1.302 0.99
274_E 277_R 1.299 0.99
220_R 272_L 1.297 0.99
206_R 210_Q 1.29 0.99
20_P 31_T 1.289 0.99
185_K 224_S 1.277 0.99
63_G 87_D 1.277 0.99
232_G 272_L 1.263 0.99
48_I 260_G 1.254 0.99
192_S 198_E 1.254 0.99
61_A 66_G 1.25 0.99
165_V 181_I 1.249 0.99
261_A 279_G 1.246 0.98
42_I 73_L 1.243 0.98
46_R 50_H 1.239 0.98
188_Q 228_Q 1.235 0.98
213_V 235_S 1.23 0.98
265_K 275_M 1.229 0.98
3_R 54_S 1.226 0.98
72_L 76_E 1.226 0.98
51_L 261_A 1.21 0.98
4_I 48_I 1.204 0.98
154_S 180_N 1.199 0.98
206_R 238_C 1.195 0.98
71_S 74_A 1.188 0.98
187_N 190_E 1.186 0.98
285_A 289_N 1.184 0.98
280_V 283_G 1.181 0.98
136_I 163_C 1.18 0.98
162_R 183_L 1.179 0.98
246_V 287_T 1.166 0.97
248_S 287_T 1.162 0.97
134_L 156_A 1.158 0.97
241_V 280_V 1.158 0.97
119_R 151_Q 1.153 0.97
224_S 258_M 1.142 0.97
225_L 229_G 1.141 0.97
53_G 267_A 1.135 0.97
61_A 70_V 1.134 0.97
178_I 181_I 1.133 0.97
45_A 55_A 1.131 0.97
207_K 210_Q 1.128 0.97
262_M 272_L 1.124 0.97
37_P 72_L 1.123 0.97
264_L 268_E 1.121 0.97
15_S 33_P 1.109 0.96
60_P 86_K 1.108 0.96
209_A 223_V 1.1 0.96
220_R 266_L 1.097 0.96
283_G 286_A 1.097 0.96
47_A 257_S 1.096 0.96
285_A 288_L 1.089 0.96
185_K 262_M 1.083 0.96
54_S 79_P 1.08 0.95
275_M 279_G 1.075 0.95
244_P 280_V 1.063 0.95
63_G 85_A 1.06 0.95
67_E 70_V 1.06 0.95
166_D 185_K 1.051 0.94
51_L 282_A 1.049 0.94
298_D 301_Q 1.041 0.94
20_P 99_A 1.038 0.94
243_P 280_V 1.038 0.94
299_D 302_K 1.036 0.94
277_R 281_A 1.036 0.94
21_Q 26_G 1.03 0.94
115_E 151_Q 1.027 0.94
5_Y 58_I 1.027 0.94
136_I 153_I 1.02 0.93
278_F 300_T 1.02 0.93
266_L 275_M 1.017 0.93
183_L 262_M 1.013 0.93
11_P 69_L 1.012 0.93
25_E 102_E 1.008 0.93
49_A 54_S 1.004 0.93
134_L 161_I 1.003 0.93
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
3umoA20.99351000.087Contact Map0.745
2jg1A40.99031000.11Contact Map0.71
2f02A20.98711000.114Contact Map0.663
3ie7A10.97091000.131Contact Map0.632
2ajrA20.98711000.134Contact Map0.667
2abqA20.97091000.137Contact Map0.75
2jg5A20.97091000.14Contact Map0.72
2rbcA10.93531000.144Contact Map0.765
2fv7A20.95151000.151Contact Map0.802
3ry7A10.94171000.154Contact Map0.755
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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