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OPENSEQ.org
WCAL - Putative colanic acid biosynthesis glycosyltransferase WcaL
UniProt: P71243 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG12652
Length: 406 (397)
Sequences: 10646
Seq/Len: 26.82
WCAL
Paralog alert: 0.74 [within 20: 0.28] - ratio of genomes with paralogs
Cluster includes: GLGA RFAB RFAG WBBK WCAC WCAI WCAL
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
265_E 269_R 4.303 1.00
226_I 299_A 4.267 1.00
255_R 282_E 3.72 1.00
242_E 271_L 3.298 1.00
2_K 31_E 3.298 1.00
123_I 403_L 3.175 1.00
244_C 256_Y 2.849 1.00
28_M 397_R 2.582 1.00
272_I 283_M 2.545 1.00
246_Q 358_R 2.456 1.00
383_R 387_E 2.433 1.00
33_E 119_A 2.421 1.00
222_P 255_R 2.401 1.00
335_L 340_P 2.341 1.00
177_L 200_A 2.305 1.00
353_P 359_A 2.304 1.00
186_A 190_Q 2.282 1.00
261_I 265_E 2.277 1.00
272_I 277_L 2.237 1.00
227_S 237_L 2.218 1.00
246_Q 361_A 2.212 1.00
4_G 35_L 2.2 1.00
259_L 295_M 2.168 1.00
250_Q 365_A 2.15 1.00
224_E 257_R 2.134 1.00
385_K 389_D 2.127 1.00
302_F 324_A 2.112 1.00
123_I 149_I 2.11 1.00
356_D 359_A 2.097 1.00
30_F 400_A 2.092 1.00
248_K 280_V 2.061 1.00
148_T 175_G 2.055 1.00
273_E 278_E 2.01 1.00
242_E 357_A 2.006 1.00
264_W 267_R 2.003 1.00
231_L 258_I 1.99 1.00
335_L 353_P 1.976 1.00
236_G 239_V 1.949 1.00
271_L 275_Y 1.944 1.00
343_V 349_G 1.941 1.00
33_E 117_F 1.938 1.00
357_A 361_A 1.936 1.00
223_L 368_S 1.931 1.00
225_I 301_V 1.931 1.00
344_E 347_K 1.915 1.00
257_R 284_P 1.912 1.00
183_D 186_A 1.899 1.00
28_M 400_A 1.894 1.00
24_A 396_N 1.877 1.00
3_V 30_F 1.85 1.00
384_E 388_H 1.842 1.00
225_I 364_L 1.839 1.00
24_A 27_D 1.83 1.00
4_G 119_A 1.83 1.00
371_D 374_E 1.828 1.00
240_A 303_L 1.827 1.00
394_V 398_E 1.82 1.00
328_G 383_R 1.818 1.00
287_K 291_E 1.817 1.00
247_L 365_A 1.802 1.00
287_K 295_M 1.772 1.00
350_W 363_R 1.76 1.00
333_S 351_L 1.756 1.00
240_A 256_Y 1.749 1.00
236_G 307_V 1.728 1.00
366_A 374_E 1.728 1.00
224_E 255_R 1.697 1.00
183_D 187_G 1.692 1.00
291_E 294_A 1.684 1.00
238_H 307_V 1.682 1.00
186_A 201_V 1.639 1.00
348_S 385_K 1.63 1.00
245_R 275_Y 1.624 1.00
270_T 274_Q 1.615 1.00
359_A 363_R 1.588 1.00
33_E 59_R 1.571 1.00
302_F 329_I 1.563 1.00
177_L 402_L 1.562 1.00
1_M 30_F 1.56 1.00
259_L 284_P 1.551 1.00
363_R 366_A 1.548 1.00
243_A 360_L 1.546 1.00
375_L 379_V 1.545 1.00
374_E 377_P 1.534 1.00
224_E 298_D 1.531 1.00
244_C 254_F 1.52 1.00
347_K 381_R 1.507 1.00
353_P 356_D 1.503 1.00
183_D 341_E 1.478 1.00
228_V 292_V 1.468 1.00
321_L 339_I 1.46 1.00
381_R 384_E 1.454 1.00
186_A 199_I 1.451 1.00
151_H 319_V 1.448 1.00
183_D 201_V 1.446 1.00
205_G 392_Q 1.443 1.00
288_P 291_E 1.438 1.00
257_R 282_E 1.435 1.00
228_V 259_L 1.433 1.00
5_F 125_H 1.43 1.00
352_V 363_R 1.425 1.00
225_I 303_L 1.42 1.00
226_I 296_L 1.419 1.00
200_A 405_A 1.397 0.99
241_I 272_I 1.387 0.99
247_L 254_F 1.384 0.99
366_A 371_D 1.382 0.99
239_V 355_N 1.377 0.99
237_L 258_I 1.377 0.99
21_Q 399_L 1.373 0.99
344_E 348_S 1.372 0.99
377_P 381_R 1.37 0.99
344_E 385_K 1.366 0.99
379_V 382_A 1.36 0.99
223_L 300_D 1.349 0.99
394_V 397_R 1.348 0.99
180_P 199_I 1.336 0.99
24_A 393_Q 1.319 0.99
4_G 114_A 1.316 0.99
398_E 401_S 1.316 0.99
299_A 329_I 1.314 0.99
2_K 33_E 1.312 0.99
5_F 25_F 1.31 0.99
350_W 366_A 1.304 0.99
5_F 22_I 1.301 0.99
179_L 399_L 1.3 0.99
372_T 375_L 1.284 0.99
271_L 274_Q 1.282 0.99
228_V 296_L 1.274 0.99
384_E 387_E 1.272 0.99
238_H 271_L 1.272 0.99
259_L 287_K 1.268 0.99
232_T 263_P 1.261 0.99
26_I 29_G 1.252 0.99
365_A 369_Q 1.248 0.99
400_A 404_Q 1.239 0.98
268_L 283_M 1.232 0.98
181_I 204_M 1.228 0.98
251_G 365_A 1.224 0.98
22_I 34_I 1.219 0.98
266_R 269_R 1.212 0.98
306_S 315_E 1.212 0.98
19_L 23_T 1.211 0.98
179_L 402_L 1.21 0.98
359_A 362_Q 1.208 0.98
225_I 244_C 1.198 0.98
292_V 296_L 1.193 0.98
333_S 343_V 1.171 0.98
202_S 402_L 1.167 0.97
207_D 210_R 1.162 0.97
125_H 151_H 1.161 0.97
246_Q 357_A 1.157 0.97
226_I 259_L 1.153 0.97
303_L 364_L 1.152 0.97
179_L 202_S 1.151 0.97
124_A 146_I 1.141 0.97
317_I 322_M 1.138 0.97
203_R 390_F 1.138 0.97
301_V 364_L 1.137 0.97
251_G 369_Q 1.136 0.97
187_G 191_K 1.133 0.97
334_T 352_V 1.128 0.97
246_Q 249_E 1.127 0.97
234_K 314_M 1.125 0.97
202_S 399_L 1.122 0.97
333_S 339_I 1.116 0.96
195_P 198_K 1.115 0.96
2_K 119_A 1.114 0.96
22_I 26_I 1.112 0.96
121_V 403_L 1.111 0.96
257_R 295_M 1.109 0.96
238_H 242_E 1.109 0.96
324_A 329_I 1.102 0.96
343_V 348_S 1.101 0.96
391_N 394_V 1.1 0.96
190_Q 196_R 1.098 0.96
145_K 176_D 1.096 0.96
5_F 32_V 1.09 0.96
329_I 379_V 1.086 0.96
380_K 384_E 1.085 0.96
247_L 364_L 1.079 0.95
24_A 28_M 1.074 0.95
180_P 186_A 1.071 0.95
223_L 301_V 1.069 0.95
125_H 149_I 1.067 0.95
203_R 341_E 1.063 0.95
296_L 327_V 1.059 0.95
224_E 300_D 1.057 0.95
241_I 277_L 1.055 0.95
376_A 380_K 1.052 0.95
337_S 340_P 1.051 0.94
235_K 315_E 1.05 0.94
211_F 327_V 1.05 0.94
395_I 398_E 1.049 0.94
226_I 295_M 1.048 0.94
302_F 320_A 1.047 0.94
284_P 287_K 1.045 0.94
239_V 307_V 1.043 0.94
352_V 359_A 1.035 0.94
338_G 341_E 1.032 0.94
332_V 364_L 1.031 0.94
241_I 281_V 1.023 0.93
296_L 320_A 1.023 0.93
28_M 396_N 1.016 0.93
219_P 222_P 1.016 0.93
17_F 319_V 1.014 0.93
362_Q 366_A 1.012 0.93
187_G 190_Q 1.01 0.93
315_E 318_P 1.008 0.93
269_R 273_E 1.004 0.93
230_R 234_K 1.003 0.93
18_V 125_H 1 0.92
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
2r60A10.91631000.201Contact Map0.707
3c48A20.90641000.229Contact Map0.806
1rzuA20.9361000.235Contact Map0.71
3s28A80.9311000.243Contact Map0.588
2qzsA10.9361000.246Contact Map0.729
3froA30.92361000.252Contact Map0.768
2jjmA120.89161000.26Contact Map0.781
3okpA10.86951000.263Contact Map0.774
2x0dA20.85711000.263Contact Map0.589
2x6qA20.8671000.264Contact Map0.772
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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