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WCAI - Putative colanic acid biosynthesis glycosyl transferase WcaI
UniProt: P32057 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG11790
Length: 407 (401)
Sequences: 17039
Seq/Len: 42.49
WCAI
Paralog alert: 0.86 [within 20: 0.38] - ratio of genomes with paralogs
Cluster includes: GLGA RFAB RFAG WBBK WCAC WCAI WCAL
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
235_L 305_G 4.478 1.00
272_K 276_E 4.06 1.00
262_I 288_Q 3.848 1.00
2_K 34_E 3.513 1.00
110_I 405_I 3.238 1.00
251_E 278_M 3.213 1.00
176_N 199_I 3.092 1.00
253_A 263_F 2.698 1.00
231_K 262_I 2.544 1.00
185_M 189_I 2.51 1.00
255_R 365_E 2.484 1.00
36_R 106_P 2.445 1.00
36_R 68_W 2.419 1.00
308_H 328_I 2.408 1.00
387_Y 391_T 2.406 1.00
339_A 346_G 2.393 1.00
31_Q 399_R 2.386 1.00
255_R 368_V 2.32 1.00
385_R 389_E 2.259 1.00
236_Y 246_L 2.248 1.00
232_K 375_L 2.242 1.00
33_H 402_I 2.223 1.00
360_E 366_A 2.211 1.00
268_Q 272_K 2.157 1.00
339_A 360_E 2.15 1.00
4_L 38_I 2.147 1.00
349_C 356_A 2.142 1.00
251_E 364_V 2.128 1.00
110_I 136_H 2.103 1.00
36_R 104_W 2.1 1.00
233_I 264_A 2.082 1.00
279_A 289_F 2.08 1.00
266_V 301_L 2.08 1.00
386_E 390_R 2.053 1.00
234_I 371_I 2.031 1.00
363_S 366_A 2.025 1.00
279_A 284_L 2.009 1.00
396_N 400_Q 2 1.00
271_G 274_R 1.989 1.00
234_I 307_C 1.981 1.00
264_A 290_F 1.96 1.00
257_R 286_N 1.952 1.00
4_L 106_P 1.948 1.00
245_G 248_N 1.945 1.00
135_L 174_V 1.936 1.00
249_V 309_L 1.929 1.00
182_R 185_M 1.925 1.00
357_V 370_G 1.906 1.00
350_E 354_G 1.905 1.00
27_W 30_A 1.904 1.00
364_V 368_V 1.899 1.00
337_I 358_C 1.884 1.00
370_G 373_Q 1.839 1.00
240_I 265_I 1.834 1.00
3_I 33_H 1.814 1.00
278_M 282_R 1.809 1.00
249_V 263_F 1.797 1.00
308_H 333_G 1.778 1.00
277_K 281_Q 1.776 1.00
355_I 387_Y 1.771 1.00
332_G 385_R 1.77 1.00
31_Q 402_I 1.763 1.00
245_G 313_K 1.749 1.00
256_L 372_R 1.728 1.00
182_R 186_N 1.725 1.00
176_N 404_D 1.713 1.00
366_A 370_G 1.711 1.00
373_Q 377_L 1.702 1.00
27_W 398_L 1.701 1.00
204_W 394_K 1.69 1.00
233_I 262_I 1.679 1.00
297_A 300_A 1.649 1.00
185_M 200_F 1.638 1.00
293_Q 301_L 1.636 1.00
27_W 395_E 1.625 1.00
237_S 298_L 1.622 1.00
254_D 282_R 1.604 1.00
233_I 304_M 1.589 1.00
185_M 198_V 1.587 1.00
36_R 66_T 1.583 1.00
1_M 33_H 1.576 1.00
294_S 297_A 1.572 1.00
182_R 347_Q 1.57 1.00
179_T 198_V 1.559 1.00
350_E 387_Y 1.55 1.00
357_V 373_Q 1.538 1.00
381_N 384_A 1.537 1.00
373_Q 380_H 1.532 1.00
354_G 383_V 1.524 1.00
293_Q 297_A 1.514 1.00
182_R 200_F 1.498 1.00
5_V 112_V 1.494 1.00
359_V 370_G 1.485 1.00
5_V 28_L 1.48 1.00
234_I 309_L 1.467 1.00
247_E 313_K 1.464 1.00
194_A 197_N 1.46 1.00
400_Q 403_N 1.453 1.00
312_Q 315_G 1.449 1.00
377_L 380_H 1.447 1.00
252_A 367_L 1.447 1.00
383_V 386_E 1.44 1.00
253_A 261_L 1.438 1.00
360_E 363_S 1.436 1.00
372_R 376_L 1.43 1.00
396_N 399_R 1.429 1.00
178_S 401_F 1.429 1.00
22_G 26_E 1.418 1.00
278_M 281_Q 1.417 1.00
5_V 25_V 1.41 1.00
266_V 290_F 1.4 0.99
305_G 333_G 1.391 0.99
235_L 266_V 1.381 0.99
178_S 404_D 1.378 0.99
243_K 314_R 1.378 0.99
237_S 302_L 1.377 0.99
264_A 288_Q 1.372 0.99
189_I 195_A 1.348 0.99
237_S 266_V 1.34 0.99
247_E 278_M 1.328 0.99
273_A 276_E 1.324 0.99
366_A 369_A 1.323 0.99
350_E 355_I 1.322 0.99
111_G 133_T 1.316 0.99
186_N 190_E 1.299 0.99
186_N 189_I 1.296 0.99
29_A 32_G 1.293 0.99
232_K 306_D 1.278 0.99
255_R 364_V 1.27 0.99
235_L 302_L 1.266 0.99
266_V 293_Q 1.266 0.99
246_L 265_I 1.265 0.99
325_L 345_L 1.264 0.99
298_L 302_L 1.258 0.99
382_T 386_E 1.254 0.99
256_L 261_L 1.253 0.99
337_I 349_C 1.246 0.98
179_T 185_M 1.245 0.98
233_I 306_D 1.244 0.98
393_D 396_N 1.242 0.98
250_I 279_A 1.238 0.98
338_T 359_V 1.235 0.98
232_K 307_C 1.235 0.98
178_S 201_F 1.231 0.98
2_K 36_R 1.23 0.98
15_T 18_G 1.229 0.98
264_A 301_L 1.223 0.98
210_F 331_V 1.221 0.98
180_I 203_N 1.22 0.98
248_N 364_V 1.21 0.98
24_M 401_F 1.2 0.98
336_V 359_V 1.2 0.98
34_E 66_T 1.18 0.98
210_F 299_P 1.176 0.98
328_I 333_G 1.173 0.98
228_P 231_K 1.154 0.97
399_R 403_N 1.146 0.97
224_Q 300_A 1.145 0.97
201_F 404_D 1.144 0.97
234_I 253_A 1.136 0.97
333_G 381_N 1.136 0.97
202_P 347_Q 1.132 0.97
309_L 371_I 1.12 0.96
337_I 345_L 1.12 0.96
386_E 389_E 1.117 0.96
132_R 175_D 1.106 0.96
27_W 31_Q 1.104 0.96
25_V 37_V 1.102 0.96
273_A 277_K 1.1 0.96
206_E 209_R 1.099 0.96
247_E 251_E 1.098 0.96
126_A 175_D 1.094 0.96
248_N 362_E 1.083 0.96
307_C 371_I 1.081 0.95
381_N 385_R 1.079 0.95
395_E 399_R 1.075 0.95
108_R 405_I 1.072 0.95
236_Y 249_V 1.069 0.95
259_E 372_R 1.068 0.95
26_E 63_G 1.064 0.95
21_T 112_V 1.057 0.95
329_L 335_A 1.057 0.95
235_L 301_L 1.054 0.95
256_L 371_I 1.053 0.95
302_L 331_V 1.052 0.95
357_V 380_H 1.05 0.94
2_K 106_P 1.048 0.94
295_Y 298_L 1.048 0.94
307_C 374_A 1.047 0.94
349_C 355_I 1.043 0.94
290_F 293_Q 1.043 0.94
255_R 258_D 1.04 0.94
207_I 393_D 1.038 0.94
179_T 188_A 1.038 0.94
23_E 26_E 1.036 0.94
351_T 370_G 1.031 0.94
233_I 305_G 1.031 0.94
241_G 270_G 1.026 0.94
138_Q 323_S 1.025 0.93
307_C 336_V 1.022 0.93
374_A 377_L 1.019 0.93
25_V 65_A 1.017 0.93
290_F 301_L 1.016 0.93
341_A 346_G 1.014 0.93
299_P 331_V 1.013 0.93
369_A 373_Q 1.011 0.93
336_V 370_G 1.01 0.93
202_P 392_L 1.007 0.93
201_F 401_F 1.007 0.93
344_E 347_Q 1.007 0.93
359_V 366_A 1.007 0.93
29_A 35_V 1.004 0.93
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
2r60A10.96311000.162Contact Map0.725
3c48A20.93861000.181Contact Map0.815
1rzuA20.96071000.185Contact Map0.715
3froA30.96071000.196Contact Map0.792
3s28A80.96811000.204Contact Map0.59
2qzsA10.96311000.204Contact Map0.722
3okpA10.88211000.239Contact Map0.769
2jjmA120.89191000.245Contact Map0.791
2iw1A10.87961000.253Contact Map0.746
3oy2A20.85751000.256Contact Map0.628
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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