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OPENSEQ.org
YTFF - Inner membrane protein YtfF
UniProt: P39314 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG12506
Length: 321 (293)
Sequences: 13842
Seq/Len: 47.24
YTFF
Paralog alert: 0.87 [within 20: 0.24] - ratio of genomes with paralogs
Cluster includes: EAMA RHTA YDDG YEDA YHBE YICL YIJE YTFF
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
85_A 163_A 3.44 1.00
128_A 301_G 2.596 1.00
254_V 258_L 2.596 1.00
81_C 165_V 2.466 1.00
7_Y 254_V 2.43 1.00
93_V 160_I 2.379 1.00
16_G 274_A 2.349 1.00
84_S 162_L 2.29 1.00
279_T 285_Q 2.199 1.00
42_G 195_A 2.189 1.00
21_G 239_A 2.173 1.00
104_V 167_V 2.149 1.00
108_F 175_L 2.125 1.00
161_V 165_V 2.114 1.00
254_V 257_Q 2.088 1.00
95_T 275_G 2.06 1.00
8_A 255_A 2.02 1.00
23_L 285_Q 1.944 1.00
118_G 178_A 1.897 1.00
105_I 265_G 1.888 1.00
16_G 278_Y 1.888 1.00
231_L 235_S 1.882 1.00
42_G 198_L 1.877 1.00
40_A 241_A 1.871 1.00
60_A 63_D 1.861 1.00
241_A 246_W 1.852 1.00
81_C 162_L 1.846 1.00
106_P 129_L 1.838 1.00
63_D 183_E 1.817 1.00
22_P 83_A 1.804 1.00
39_L 198_L 1.793 1.00
110_N 120_L 1.789 1.00
13_L 274_A 1.783 1.00
94_S 163_A 1.749 1.00
109_A 116_R 1.735 1.00
109_A 118_G 1.708 1.00
100_T 104_V 1.702 1.00
17_L 239_A 1.697 1.00
89_T 160_I 1.629 1.00
274_A 278_Y 1.6 1.00
121_A 124_K 1.585 1.00
288_P 291_T 1.584 1.00
68_L 196_Q 1.579 1.00
81_C 169_C 1.579 1.00
130_I 134_I 1.569 1.00
260_P 263_I 1.551 1.00
46_L 191_M 1.544 1.00
278_Y 282_L 1.527 1.00
185_P 188_H 1.519 1.00
132_I 269_V 1.518 1.00
289_L 293_S 1.511 1.00
89_T 93_V 1.502 1.00
61_R 65_L 1.493 1.00
108_F 172_W 1.468 1.00
35_M 201_L 1.454 1.00
303_V 307_R 1.453 1.00
89_T 159_G 1.445 1.00
35_M 198_L 1.431 1.00
138_C 297_L 1.429 1.00
296_A 300_I 1.424 1.00
104_V 168_V 1.42 1.00
91_A 279_T 1.419 1.00
292_L 296_A 1.414 1.00
28_Y 233_F 1.402 0.99
33_Q 240_I 1.392 0.99
43_L 47_P 1.378 0.99
230_P 233_F 1.361 0.99
62_R 65_L 1.356 0.99
71_T 196_Q 1.356 0.99
47_P 50_W 1.349 0.99
125_L 130_I 1.347 0.99
11_A 255_A 1.342 0.99
287_P 292_L 1.342 0.99
14_M 243_L 1.335 0.99
69_M 176_R 1.326 0.99
88_R 158_S 1.325 0.99
6_L 10_L 1.324 0.99
122_W 126_A 1.324 0.99
42_G 191_M 1.322 0.99
84_S 88_R 1.321 0.99
96_M 295_I 1.317 0.99
7_Y 251_C 1.298 0.99
92_P 276_L 1.297 0.99
65_L 69_M 1.276 0.99
77_I 169_C 1.274 0.99
128_A 305_A 1.271 0.99
21_G 236_L 1.269 0.99
232_V 235_S 1.268 0.99
138_C 290_M 1.264 0.99
179_R 183_E 1.263 0.99
120_L 125_L 1.25 0.99
105_I 261_T 1.246 0.98
139_V 294_G 1.244 0.98
91_A 283_R 1.244 0.98
108_F 112_L 1.244 0.98
238_V 241_A 1.236 0.98
35_M 39_L 1.231 0.98
104_V 171_A 1.229 0.98
157_T 161_V 1.225 0.98
44_I 246_W 1.216 0.98
17_L 278_Y 1.21 0.98
177_N 253_N 1.206 0.98
97_I 167_V 1.205 0.98
72_M 200_T 1.203 0.98
29_P 32_L 1.198 0.98
171_A 175_L 1.195 0.98
86_I 94_S 1.195 0.98
231_L 234_I 1.195 0.98
243_L 247_V 1.188 0.98
112_L 116_R 1.188 0.98
90_G 93_V 1.18 0.98
11_A 15_W 1.178 0.98
66_T 176_R 1.17 0.97
118_G 124_K 1.169 0.97
256_S 260_P 1.167 0.97
280_F 287_P 1.157 0.97
108_F 168_V 1.151 0.97
20_V 278_Y 1.149 0.97
91_A 285_Q 1.142 0.97
44_I 241_A 1.142 0.97
27_E 233_F 1.138 0.97
178_A 260_P 1.134 0.97
100_T 136_L 1.131 0.97
14_M 251_C 1.123 0.97
167_V 171_A 1.12 0.96
44_I 242_V 1.119 0.96
110_N 119_K 1.118 0.96
82_L 86_I 1.114 0.96
235_S 239_A 1.111 0.96
46_L 50_W 1.111 0.96
256_S 264_L 1.109 0.96
105_I 174_A 1.108 0.96
84_S 87_Q 1.104 0.96
97_I 164_L 1.102 0.96
41_L 194_T 1.095 0.96
270_F 274_A 1.092 0.96
5_V 267_L 1.091 0.96
97_I 163_A 1.091 0.96
112_L 117_D 1.088 0.96
178_A 261_T 1.085 0.96
24_I 236_L 1.085 0.96
105_I 175_L 1.083 0.96
15_W 252_W 1.081 0.95
135_G 294_G 1.081 0.95
108_F 171_A 1.08 0.95
116_R 175_L 1.075 0.95
88_R 159_G 1.073 0.95
15_W 248_G 1.064 0.95
103_V 132_I 1.062 0.95
273_L 295_I 1.059 0.95
31_M 80_F 1.059 0.95
234_I 238_V 1.052 0.95
199_V 202_P 1.05 0.94
108_F 111_L 1.049 0.94
134_I 138_C 1.048 0.94
111_L 117_D 1.046 0.94
179_R 182_R 1.046 0.94
96_M 137_A 1.045 0.94
11_A 251_C 1.045 0.94
20_V 282_L 1.042 0.94
61_R 64_W 1.041 0.94
73_M 172_W 1.04 0.94
135_G 298_L 1.039 0.94
3_S 258_L 1.032 0.94
22_P 86_I 1.022 0.93
259_L 264_L 1.019 0.93
170_W 174_A 1.018 0.93
260_P 264_L 1.017 0.93
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
2i68A20.358398.90.772Contact Map0.012
3b5dA20.336498.10.828Contact Map0.053
2kluA10.143317.70.944Contact Map0.346
2cfqA10.57328.60.952Contact Map0.348
2xutA30.7576.60.954Contact Map0.151
3j01B10.19636.30.954Contact Map0.047
3wdoA10.73214.40.958Contact Map0.193
1pw4A10.90343.30.96Contact Map0.174
2kncA10.14953.10.961Contact Map0.361
4il6R10.10592.80.962Contact Map0.556
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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