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OPENSEQ.org
EAMA - Probable amino-acid metabolite efflux pump
UniProt: P31125 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG11639
Length: 299 (281)
Sequences: 13114
Seq/Len: 46.67
EAMA
Paralog alert: 0.83 [within 20: 0.18] - ratio of genomes with paralogs
Cluster includes: EAMA RHTA YDDG YEDA YHBE YICL YIJE YTFF
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
79_A 148_T 3.513 1.00
119_G 281_G 2.707 1.00
44_A 183_L 2.62 1.00
234_T 238_R 2.494 1.00
17_G 254_V 2.477 1.00
8_L 234_T 2.323 1.00
99_F 152_A 2.251 1.00
259_A 265_E 2.245 1.00
75_F 150_A 2.245 1.00
22_V 219_Y 2.212 1.00
88_L 145_F 2.195 1.00
78_C 147_L 2.158 1.00
103_L 160_I 2.138 1.00
234_T 237_G 2.11 1.00
146_M 150_A 2.068 1.00
37_G 190_F 2.064 1.00
90_S 255_G 2.004 1.00
9_A 235_L 1.977 1.00
44_A 186_I 1.975 1.00
24_K 265_E 1.97 1.00
100_T 245_A 1.935 1.00
42_L 221_A 1.925 1.00
110_E 163_K 1.918 1.00
221_A 226_I 1.906 1.00
17_G 258_S 1.897 1.00
41_M 186_I 1.896 1.00
211_M 215_L 1.874 1.00
14_V 254_V 1.816 1.00
105_A 112_L 1.813 1.00
23_I 77_F 1.782 1.00
101_I 120_I 1.781 1.00
75_F 147_L 1.738 1.00
104_G 108_F 1.708 1.00
18_L 219_Y 1.703 1.00
95_A 99_F 1.682 1.00
89_A 148_T 1.663 1.00
258_S 262_L 1.641 1.00
104_G 110_E 1.634 1.00
84_M 145_F 1.605 1.00
103_L 157_C 1.58 1.00
30_M 213_T 1.539 1.00
269_G 273_L 1.538 1.00
254_V 258_S 1.527 1.00
268_T 271_Q 1.526 1.00
123_A 249_L 1.522 1.00
188_P 191_V 1.515 1.00
37_G 186_I 1.502 1.00
113_H 116_Q 1.5 1.00
121_A 125_F 1.498 1.00
15_V 223_V 1.49 1.00
84_M 88_L 1.483 1.00
99_F 153_F 1.463 1.00
240_E 243_R 1.457 1.00
272_F 276_V 1.454 1.00
26_G 35_L 1.452 1.00
86_A 259_A 1.451 1.00
75_F 154_S 1.438 1.00
35_L 220_L 1.404 0.99
156_A 160_I 1.404 0.99
45_F 49_F 1.395 0.99
12_V 235_L 1.377 0.99
18_L 258_S 1.371 0.99
84_M 144_G 1.366 0.99
21_V 258_S 1.365 0.99
71_A 154_S 1.362 0.99
129_V 277_L 1.353 0.99
210_D 213_T 1.333 0.99
283_Y 287_F 1.317 0.99
162_N 233_G 1.311 0.99
99_F 156_A 1.311 0.99
126_G 274_G 1.308 0.99
119_G 285_N 1.305 0.99
212_T 215_L 1.304 0.99
130_L 274_G 1.302 0.99
114_G 118_A 1.3 0.99
82_F 143_L 1.297 0.99
48_I 179_I 1.294 0.99
78_C 82_F 1.29 0.99
92_V 152_A 1.288 0.99
87_G 256_L 1.281 0.99
46_P 226_I 1.274 0.99
129_V 270_L 1.271 0.99
37_G 193_S 1.263 0.99
112_L 117_L 1.252 0.99
110_E 116_Q 1.248 0.99
44_A 179_I 1.242 0.98
37_G 41_M 1.242 0.98
31_P 34_M 1.241 0.98
22_V 216_S 1.24 0.98
276_V 280_T 1.239 0.98
218_M 221_A 1.218 0.98
46_P 222_F 1.215 0.98
267_L 272_F 1.213 0.98
211_M 214_I 1.208 0.98
152_A 156_A 1.201 0.98
223_V 227_V 1.198 0.98
187_I 191_V 1.198 0.98
117_L 121_A 1.196 0.98
62_L 184_I 1.195 0.98
85_P 88_L 1.194 0.98
29_N 213_T 1.191 0.98
91_L 275_A 1.182 0.98
78_C 81_N 1.181 0.98
236_L 240_E 1.18 0.98
173_A 176_S 1.175 0.98
163_K 241_T 1.175 0.98
95_A 127_V 1.171 0.98
103_L 107_T 1.163 0.97
100_T 159_N 1.158 0.97
76_L 80_I 1.154 0.97
12_V 16_W 1.15 0.97
8_L 231_I 1.145 0.97
80_I 89_A 1.142 0.97
184_I 188_P 1.141 0.97
239_Y 244_V 1.137 0.97
65_G 184_I 1.13 0.97
100_T 241_T 1.128 0.97
105_A 111_R 1.128 0.97
250_L 254_V 1.127 0.97
253_V 275_A 1.123 0.97
92_V 149_L 1.121 0.97
86_A 265_E 1.12 0.96
92_V 148_T 1.117 0.96
98_F 123_A 1.114 0.96
163_K 240_E 1.111 0.96
16_W 228_G 1.108 0.96
126_G 278_I 1.103 0.96
119_G 284_I 1.097 0.96
7_V 11_L 1.093 0.96
46_P 221_A 1.093 0.96
21_V 262_L 1.092 0.96
29_N 210_D 1.09 0.96
142_M 146_M 1.084 0.96
215_L 219_Y 1.082 0.96
103_L 153_F 1.082 0.96
16_W 232_W 1.08 0.95
6_G 247_L 1.076 0.95
260_A 267_L 1.069 0.95
214_I 218_M 1.061 0.95
33_L 74_A 1.06 0.95
91_L 128_L 1.059 0.95
21_V 259_A 1.057 0.95
106_F 109_G 1.053 0.95
256_L 267_L 1.051 0.94
24_K 28_H 1.049 0.94
164_K 167_S 1.044 0.94
25_V 216_S 1.043 0.94
36_A 189_F 1.033 0.94
86_A 263_L 1.032 0.94
47_A 226_I 1.031 0.94
26_G 216_S 1.031 0.94
79_A 89_A 1.027 0.94
125_F 129_V 1.026 0.94
164_K 168_H 1.023 0.93
13_V 254_V 1.019 0.93
155_W 159_N 1.014 0.93
111_R 115_K 1.009 0.93
240_E 244_V 1.007 0.93
54_P 173_A 1.006 0.93
26_G 213_T 1.004 0.93
49_F 52_A 1.002 0.92
103_L 106_F 1.001 0.92
103_L 156_A 1.001 0.92
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
2i68A20.377998.90.766Contact Map0.014
3b5dA20.361298.10.818Contact Map0.057
4j05A20.93984.50.956Contact Map0.206
2kluA10.14724.40.956Contact Map0.375
4h44G10.12043.80.957Contact Map0.455
4hg6A10.3113.10.959Contact Map0.355
2cfqA10.59532.80.96Contact Map0.322
3j01B10.2042.50.961Contact Map0.04
4il6R10.11372.40.961Contact Map0.558
4knfA50.20742.40.961Contact Map0.386
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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