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OPENSEQ.org
PSPF - Psp operon transcriptional activator
UniProt: P37344 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG12344
Length: 325 (320)
Sequences: 9725
Seq/Len: 30.39
PSPF
Paralog alert: 0.78 [within 20: 0.34] - ratio of genomes with paralogs
Cluster includes: ATOC DHAR FHLA GLRR HYFR NORR NTRC PRPR PSPF RTCR TYRR YGEV ZRAR
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
155_M 160_T 3.707 1.00
298_G 308_L 3.694 1.00
37_E 180_R 3.29 1.00
81_E 91_R 3.248 1.00
95_R 130_E 3.12 1.00
154_A 158_E 2.878 1.00
70_E 115_M 2.836 1.00
128_E 137_P 2.797 1.00
129_L 140_V 2.677 1.00
196_Q 230_K 2.645 1.00
97_E 131_R 2.596 1.00
150_A 155_M 2.513 1.00
22_V 45_I 2.457 1.00
288_K 292_Q 2.436 1.00
66_A 118_E 2.43 1.00
24_H 241_G 2.398 1.00
105_F 147_A 2.333 1.00
297_Q 308_L 2.285 1.00
81_E 134_G 2.267 1.00
96_F 123_V 2.254 1.00
187_M 214_R 2.226 1.00
128_E 139_Q 2.224 1.00
298_G 305_A 2.152 1.00
151_D 154_A 2.148 1.00
222_W 232_V 2.146 1.00
290_L 294_S 2.122 1.00
130_E 137_P 2.12 1.00
304_R 307_E 2.017 1.00
287_E 319_L 1.978 1.00
125_E 230_K 1.941 1.00
187_M 218_L 1.928 1.00
74_D 119_K 1.867 1.00
101_G 141_N 1.851 1.00
155_M 158_E 1.822 1.00
154_A 157_N 1.753 1.00
55_R 141_N 1.741 1.00
190_A 233_V 1.74 1.00
43_E 122_R 1.721 1.00
314_H 318_A 1.72 1.00
190_A 209_F 1.718 1.00
209_F 233_V 1.706 1.00
220_Y 223_P 1.679 1.00
111_T 150_A 1.674 1.00
213_A 217_L 1.671 1.00
35_I 175_Q 1.661 1.00
294_S 298_G 1.627 1.00
12_A 184_S 1.627 1.00
45_I 176_L 1.623 1.00
73_L 116_V 1.619 1.00
134_G 138_L 1.606 1.00
10_G 15_F 1.577 1.00
124_I 144_L 1.576 1.00
82_A 89_Q 1.564 1.00
30_K 234_E 1.562 1.00
210_T 213_A 1.558 1.00
192_Y 196_Q 1.547 1.00
11_E 188_L 1.546 1.00
183_E 187_M 1.516 1.00
152_L 161_F 1.516 1.00
183_E 221_R 1.499 1.00
40_T 176_L 1.495 1.00
100_D 140_V 1.492 1.00
220_Y 228_E 1.484 1.00
80_H 85_F 1.473 1.00
35_I 173_V 1.464 1.00
303_K 313_Y 1.463 1.00
81_E 131_R 1.461 1.00
82_A 90_K 1.459 1.00
123_V 129_L 1.458 1.00
191_E 214_R 1.457 1.00
312_T 315_Q 1.442 1.00
286_Q 290_L 1.442 1.00
96_F 104_L 1.428 1.00
301_N 304_R 1.423 1.00
23_S 52_L 1.42 1.00
210_T 246_P 1.419 1.00
129_L 139_Q 1.413 1.00
25_L 172_D 1.391 0.99
110_A 148_T 1.39 0.99
97_E 138_L 1.39 0.99
100_D 141_N 1.383 0.99
58_G 101_G 1.357 0.99
303_K 307_E 1.357 0.99
18_V 174_V 1.354 0.99
292_Q 296_Q 1.345 0.99
15_F 19_L 1.342 0.99
183_E 186_I 1.341 0.99
92_H 135_S 1.34 0.99
284_M 322_K 1.336 0.99
70_E 113_P 1.334 0.99
294_S 305_A 1.329 0.99
29_D 55_R 1.326 0.99
197_M 237_V 1.32 0.99
146_C 169_L 1.319 0.99
314_H 317_R 1.314 0.99
200_E 234_E 1.314 0.99
205_L 208_G 1.311 0.99
295_L 305_A 1.31 0.99
302_Q 317_R 1.304 0.99
189_M 193_F 1.304 0.99
30_K 201_I 1.298 0.99
93_P 97_E 1.297 0.99
214_R 218_L 1.294 0.99
47_S 62_S 1.289 0.99
312_T 318_A 1.282 0.99
213_A 245_Y 1.278 0.99
215_E 219_N 1.271 0.99
283_Q 286_Q 1.268 0.99
18_V 45_I 1.263 0.99
175_Q 181_E 1.263 0.99
48_R 52_L 1.249 0.99
131_R 134_G 1.247 0.99
294_S 309_L 1.235 0.98
208_G 244_D 1.228 0.98
293_L 297_Q 1.22 0.98
193_F 230_K 1.22 0.98
19_L 22_V 1.208 0.98
33_L 146_C 1.202 0.98
82_A 91_R 1.201 0.98
197_M 233_V 1.2 0.98
20_E 23_S 1.199 0.98
17_E 20_E 1.191 0.98
10_G 19_L 1.189 0.98
9_L 192_Y 1.189 0.98
304_R 308_L 1.18 0.98
194_A 237_V 1.175 0.98
193_F 197_M 1.174 0.98
73_L 77_L 1.164 0.97
187_M 190_A 1.145 0.97
8_L 15_F 1.144 0.97
153_P 157_N 1.139 0.97
194_A 209_F 1.132 0.97
302_Q 313_Y 1.131 0.97
198_C 204_P 1.13 0.97
318_A 322_K 1.128 0.97
30_K 238_Y 1.12 0.96
30_K 171_F 1.114 0.96
157_N 163_A 1.112 0.96
35_I 153_P 1.111 0.96
294_S 308_L 1.095 0.96
8_L 45_I 1.094 0.96
60_F 102_G 1.093 0.96
96_F 142_V 1.091 0.96
29_D 200_E 1.082 0.96
232_V 236_S 1.079 0.95
112_A 116_V 1.075 0.95
315_Q 319_L 1.075 0.95
66_A 111_T 1.069 0.95
76_E 92_H 1.066 0.95
184_S 218_L 1.059 0.95
85_F 88_A 1.057 0.95
183_E 218_L 1.056 0.95
207_P 243_S 1.052 0.95
37_E 178_P 1.049 0.94
97_E 129_L 1.047 0.94
301_N 313_Y 1.045 0.94
10_G 188_L 1.041 0.94
317_R 321_K 1.039 0.94
313_Y 317_R 1.038 0.94
210_T 244_D 1.037 0.94
62_S 122_R 1.036 0.94
131_R 138_L 1.03 0.94
31_P 127_G 1.028 0.94
15_F 18_V 1.021 0.93
95_R 98_R 1.02 0.93
100_D 142_V 1.019 0.93
230_K 234_E 1.015 0.93
291_L 316_F 1.011 0.93
98_R 135_S 1.01 0.93
21_Q 239_R 1.008 0.93
13_N 17_E 1.005 0.93
29_D 54_S 1.005 0.93
34_I 45_I 1.002 0.92
252_I 255_F 1.001 0.92
209_F 214_R 1 0.92
127_G 139_Q 1 0.92
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
4l4uA10.94461000.02Contact Map0.646
1ojlA60.93541000.214Contact Map0.736
1ny5A20.78771000.233Contact Map0.73
3dzdA20.75381000.281Contact Map0.718
2bjvA10.81541000.512Contact Map0.675
4fwgA10.87081000.545Contact Map0.346
1hqcA20.81541000.569Contact Map0.524
1qvrA30.83691000.576Contact Map0.33
1in4A10.81851000.585Contact Map0.516
1r6bX10.83691000.586Contact Map0.408
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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