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OPENSEQ.org
INSF4 - Transposase InsF for insertion sequence IS3D
UniProt: P0CF82 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG60023
Length: 288 (282)
Sequences: 4002
Seq/Len: 14.19
INSF4
Paralog alert: 0.71 [within 20: 0.17] - ratio of genomes with paralogs
Cluster includes: INSD1 INSD2 INSD3 INSD4 INSD5 INSD6 INSD8 INSF1 INSF2 INSF3 INSF4 INSF5 INSK INSO1 INSO2 YAGA
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
75_A 281_Q 3.04 1.00
129_N 132_W 2.613 1.00
150_V 234_F 2.567 1.00
178_Q 208_Q 2.533 1.00
52_V 76_Q 2.501 1.00
277_L 285_K 2.422 1.00
53_V 69_L 2.397 1.00
133_A 230_V 2.345 1.00
50_D 92_Q 2.333 1.00
127_G 130_Q 2.305 1.00
262_E 267_R 2.256 1.00
248_I 252_I 2.167 1.00
25_A 28_G 2.146 1.00
278_S 281_Q 2.138 1.00
50_D 91_R 2.132 1.00
158_A 280_E 2.112 1.00
163_S 179_M 2.093 1.00
247_F 256_T 2.087 1.00
81_N 84_T 2.052 1.00
71_D 276_G 2.016 1.00
267_R 280_E 1.988 1.00
134_G 194_T 1.9 1.00
126_S 130_Q 1.874 1.00
138_Y 145_W 1.872 1.00
249_S 252_I 1.85 1.00
52_V 55_A 1.837 1.00
157_R 271_H 1.834 1.00
132_W 180_A 1.767 1.00
150_V 230_V 1.734 1.00
161_G 176_A 1.729 1.00
132_W 151_V 1.691 1.00
163_S 175_D 1.683 1.00
70_T 74_R 1.682 1.00
237_L 257_V 1.682 1.00
72_E 270_R 1.651 1.00
149_A 200_Y 1.647 1.00
148_L 237_L 1.646 1.00
274_C 278_S 1.645 1.00
206_Q 216_G 1.635 1.00
70_T 85_V 1.633 1.00
133_A 154_L 1.609 1.00
240_E 269_R 1.577 1.00
64_Y 68_R 1.57 1.00
53_V 88_S 1.559 1.00
235_H 239_V 1.546 1.00
168_M 199_Q 1.541 1.00
66_A 86_A 1.532 1.00
277_L 282_F 1.531 1.00
125_A 131_K 1.523 1.00
141_T 144_G 1.522 1.00
253_M 257_V 1.522 1.00
158_A 283_E 1.51 1.00
136_I 199_Q 1.501 1.00
80_F 85_V 1.496 1.00
56_A 69_L 1.491 1.00
220_A 226_D 1.488 1.00
241_C 256_T 1.481 1.00
14_S 17_A 1.477 1.00
241_C 247_F 1.471 1.00
134_G 192_V 1.469 1.00
132_W 190_V 1.467 1.00
84_T 87_A 1.453 1.00
190_V 214_L 1.436 1.00
192_V 205_Y 1.432 1.00
27_S 30_Y 1.431 1.00
232_S 235_H 1.411 1.00
170_A 174_C 1.409 1.00
152_I 159_V 1.407 0.99
272_S 276_G 1.402 0.99
154_L 229_C 1.383 0.99
42_R 46_R 1.373 0.99
134_G 200_Y 1.371 0.99
266_N 269_R 1.356 0.99
75_A 270_R 1.352 0.99
66_A 82_V 1.345 0.99
56_A 72_E 1.333 0.99
219_S 226_D 1.325 0.99
68_R 240_E 1.322 0.99
68_R 272_S 1.305 0.99
16_K 26_R 1.301 0.99
5_F 9_H 1.299 0.99
148_L 162_W 1.295 0.99
196_R 221_K 1.282 0.99
207_A 211_R 1.261 0.99
59_R 268_W 1.26 0.99
137_T 238_K 1.259 0.99
165_S 172_L 1.251 0.99
86_A 90_R 1.251 0.99
46_R 84_T 1.248 0.99
156_S 283_E 1.246 0.98
225_Y 228_A 1.245 0.98
146_L 250_R 1.243 0.98
52_V 78_Y 1.242 0.98
72_E 76_Q 1.241 0.98
220_A 223_C 1.239 0.98
133_A 229_C 1.237 0.98
50_D 88_S 1.236 0.98
122_D 131_K 1.236 0.98
46_R 49_C 1.233 0.98
119_L 131_K 1.227 0.98
194_T 216_G 1.22 0.98
26_R 30_Y 1.217 0.98
131_K 154_L 1.213 0.98
168_M 198_G 1.208 0.98
209_L 214_L 1.207 0.98
88_S 92_Q 1.205 0.98
45_F 49_C 1.203 0.98
50_D 54_L 1.203 0.98
57_F 94_L 1.187 0.98
149_A 176_A 1.186 0.98
208_Q 212_H 1.185 0.98
219_S 227_N 1.184 0.98
196_R 218_M 1.182 0.98
152_I 230_V 1.181 0.98
209_L 216_G 1.174 0.98
138_Y 147_Y 1.171 0.98
181_L 214_L 1.17 0.97
15_I 30_Y 1.169 0.97
166_P 250_R 1.167 0.97
24_V 28_G 1.16 0.97
105_V 108_R 1.153 0.97
274_C 279_P 1.149 0.97
70_T 82_V 1.145 0.97
159_V 261_I 1.141 0.97
74_R 79_P 1.14 0.97
181_L 212_H 1.135 0.97
117_N 219_S 1.125 0.97
198_G 202_S 1.122 0.97
274_C 282_F 1.12 0.96
159_V 233_F 1.12 0.96
148_L 164_M 1.119 0.96
55_A 59_R 1.102 0.96
271_H 274_C 1.102 0.96
151_V 180_A 1.097 0.96
129_N 184_R 1.093 0.96
139_L 257_V 1.087 0.96
45_F 84_T 1.085 0.96
7_E 10_Q 1.076 0.95
83_K 87_A 1.072 0.95
262_E 268_W 1.072 0.95
193_H 226_D 1.065 0.95
19_C 29_W 1.055 0.95
107_Y 110_H 1.054 0.95
278_S 284_N 1.051 0.94
254_R 258_F 1.045 0.94
72_E 75_A 1.043 0.94
193_H 217_S 1.042 0.94
54_L 92_Q 1.038 0.94
127_G 155_W 1.035 0.94
83_K 86_A 1.034 0.94
174_C 208_Q 1.033 0.94
49_C 53_V 1.03 0.94
60_S 264_D 1.026 0.94
232_S 236_S 1.023 0.93
170_A 202_S 1.013 0.93
57_F 62_Q 1.002 0.92
165_S 169_T 1 0.92
31_T 35_R 1 0.92
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
4fcyA20.97571000.401Contact Map0.465
3hotA20.94441000.504Contact Map0.626
1bcoA10.60071000.531Contact Map0.512
4fw1A20.57291000.575Contact Map0.7
3av9A20.61111000.584Contact Map0.44
3oymA20.90621000.586Contact Map0.585
3kksA20.51391000.593Contact Map0.598
3l3uA20.54171000.595Contact Map0.636
1c6vA40.54171000.595Contact Map0.715
1ex4A20.54171000.597Contact Map0.592
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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