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OPENSEQ.org
K1PF - 1-phosphofructokinase
UniProt: P0AEW9 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG10337
Length: 312 (301)
Sequences: 9583
Seq/Len: 31.84
K1PF
Paralog alert: 0.85 [within 20: 0.18] - ratio of genomes with paralogs
Cluster includes: FRLD K1PF K6PF2 KDGK PSUK RBSK YDJH YEGV YEII YIHV
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
54_I 263_Y 2.959 1.00
270_S 273_H 2.905 1.00
231_W 275_L 2.737 1.00
49_L 54_I 2.639 1.00
57_T 80_A 2.352 1.00
229_A 257_M 2.35 1.00
264_G 269_E 2.313 1.00
136_C 165_I 2.313 1.00
51_D 77_L 2.304 1.00
165_I 262_I 2.265 1.00
295_D 298_Q 2.231 1.00
155_T 180_A 2.189 1.00
213_R 232_V 2.179 1.00
71_Q 86_V 2.167 1.00
68_D 86_V 2.161 1.00
4_R 133_D 2.153 1.00
224_L 230_L 2.136 1.00
256_S 281_V 2.109 1.00
264_G 277_L 2.09 1.00
288_Q 298_Q 2.084 1.00
154_M 164_I 2.062 1.00
182_W 218_A 2.041 1.00
48_V 259_G 2.033 1.00
6_A 132_F 2.022 1.00
61_F 117_D 1.956 1.00
269_E 273_H 1.933 1.00
188_R 224_L 1.908 1.00
72_Q 76_E 1.898 1.00
260_G 281_V 1.888 1.00
152_D 155_T 1.851 1.00
296_R 300_A 1.837 1.00
272_E 276_R 1.808 1.00
157_L 162_P 1.77 1.00
261_L 274_T 1.766 1.00
238_W 272_E 1.737 1.00
282_A 286_V 1.735 1.00
47_K 77_L 1.723 1.00
163_C 182_W 1.722 1.00
267_M 296_R 1.702 1.00
229_A 279_T 1.675 1.00
249_S 290_N 1.67 1.00
64_K 68_D 1.667 1.00
224_L 239_I 1.648 1.00
7_T 46_A 1.647 1.00
156_R 160_Q 1.646 1.00
75_S 81_N 1.64 1.00
185_K 258_V 1.622 1.00
73_L 76_E 1.622 1.00
151_T 155_T 1.599 1.00
158_R 182_W 1.576 1.00
71_Q 75_S 1.573 1.00
130_G 160_Q 1.571 1.00
134_M 262_I 1.564 1.00
49_L 263_Y 1.561 1.00
221_V 261_L 1.555 1.00
253_A 286_V 1.547 1.00
38_A 70_F 1.542 1.00
261_L 278_A 1.541 1.00
201_E 204_D 1.541 1.00
211_A 214_E 1.524 1.00
68_D 72_Q 1.51 1.00
152_D 156_R 1.509 1.00
181_P 184_V 1.5 1.00
36_L 69_G 1.493 1.00
210_H 214_E 1.489 1.00
204_D 207_E 1.479 1.00
64_K 87_Q 1.476 1.00
4_R 55_D 1.471 1.00
71_Q 84_Q 1.468 1.00
183_L 219_H 1.463 1.00
156_R 159_S 1.463 1.00
5_V 49_L 1.455 1.00
245_V 287_S 1.454 1.00
243_P 280_A 1.452 1.00
167_D 223_S 1.447 1.00
134_M 163_C 1.44 1.00
240_A 275_L 1.422 1.00
163_C 218_A 1.411 1.00
230_L 239_I 1.411 1.00
209_A 232_V 1.408 1.00
134_M 165_I 1.4 0.99
261_L 265_L 1.4 0.99
243_P 276_R 1.397 0.99
155_T 159_S 1.396 0.99
211_A 215_Q 1.39 0.99
191_L 222_I 1.38 0.99
260_G 277_L 1.373 0.99
168_S 186_P 1.359 0.99
224_L 227_E 1.358 0.99
229_A 275_L 1.356 0.99
233_N 236_G 1.351 0.99
12_P 60_G 1.332 0.99
206_I 210_H 1.317 0.99
219_H 271_S 1.312 0.99
219_H 233_N 1.309 0.99
231_W 238_W 1.309 0.99
219_H 261_L 1.298 0.99
114_T 117_D 1.286 0.99
49_L 56_V 1.278 0.99
273_H 276_R 1.273 0.99
50_K 79_I 1.272 0.99
188_R 227_E 1.27 0.99
21_P 32_K 1.257 0.99
49_L 259_G 1.252 0.99
185_K 223_S 1.244 0.98
52_L 260_G 1.244 0.98
47_K 51_D 1.243 0.98
213_R 234_A 1.243 0.98
166_F 181_P 1.242 0.98
192_E 198_K 1.24 0.98
231_W 271_S 1.239 0.98
64_K 88_G 1.233 0.98
260_G 278_A 1.233 0.98
158_R 180_A 1.232 0.98
264_G 274_T 1.226 0.98
73_L 77_L 1.212 0.98
43_I 74_F 1.211 0.98
62_L 67_Q 1.203 0.98
279_T 282_A 1.2 0.98
247_V 286_V 1.2 0.98
206_I 237_E 1.189 0.98
224_L 228_G 1.184 0.98
137_V 164_I 1.181 0.98
72_Q 75_S 1.179 0.98
245_V 286_V 1.172 0.98
163_C 183_L 1.171 0.98
38_A 73_L 1.17 0.97
284_L 288_Q 1.169 0.97
119_E 152_D 1.164 0.97
209_A 222_I 1.164 0.97
261_L 271_S 1.152 0.97
135_V 157_L 1.15 0.97
46_A 56_V 1.143 0.97
55_D 80_A 1.14 0.97
62_L 71_Q 1.139 0.97
187_N 190_E 1.133 0.97
16_L 34_T 1.131 0.97
54_I 266_L 1.129 0.97
223_S 257_M 1.114 0.96
21_P 99_K 1.113 0.96
48_V 256_S 1.112 0.96
207_E 210_H 1.11 0.96
284_L 287_S 1.109 0.96
61_F 87_Q 1.105 0.96
240_A 279_T 1.104 0.96
197_R 211_A 1.095 0.96
263_Y 267_M 1.085 0.96
185_K 261_L 1.085 0.96
219_H 265_L 1.083 0.96
167_D 185_K 1.076 0.95
282_A 285_A 1.069 0.95
64_K 86_V 1.068 0.95
243_P 279_T 1.06 0.95
129_L 162_P 1.039 0.94
7_T 42_G 1.039 0.94
68_D 71_Q 1.038 0.94
183_L 261_L 1.037 0.94
135_V 162_P 1.035 0.94
242_P 279_T 1.035 0.94
14_Y 69_G 1.02 0.93
276_R 280_A 1.02 0.93
298_Q 301_A 1.019 0.93
274_T 278_A 1.018 0.93
137_V 154_M 1.017 0.93
50_K 55_D 1.012 0.93
6_A 59_G 1.011 0.93
52_L 281_V 1.002 0.92
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
2jg1A40.99361000.137Contact Map0.713
2f02A20.99361000.15Contact Map0.66
2ajrA20.99361000.153Contact Map0.656
3ie7A10.97761000.156Contact Map0.648
3umoA20.97761000.166Contact Map0.758
2jg5A20.97441000.174Contact Map0.712
2abqA20.97441000.178Contact Map0.73
3ry7A10.93911000.183Contact Map0.76
2fv7A20.94551000.191Contact Map0.808
1vm7A20.92951000.192Contact Map0.817
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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