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OPENSEQ.org
PSUK - Pseudouridine kinase
UniProt: P30235 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG11646
Length: 313 (304)
Sequences: 9676
Seq/Len: 31.83
PSUK
Paralog alert: 0.85 [within 20: 0.18] - ratio of genomes with paralogs
Cluster includes: FRLD K1PF K6PF2 KDGK PSUK RBSK YDJH YEGV YEII YIHV
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
55_N 264_S 2.943 1.00
271_P 274_E 2.9 1.00
6_Y 136_K 2.807 1.00
65_S 90_P 2.638 1.00
231_Y 276_V 2.591 1.00
50_L 55_N 2.563 1.00
8_V 135_A 2.562 1.00
139_V 162_F 2.547 1.00
73_L 88_I 2.531 1.00
70_Q 88_I 2.429 1.00
229_V 258_M 2.34 1.00
58_W 82_Y 2.33 1.00
162_F 263_A 2.316 1.00
265_C 270_M 2.279 1.00
52_L 79_S 2.275 1.00
154_D 180_Q 2.272 1.00
6_Y 56_K 2.193 1.00
153_L 161_V 2.13 1.00
224_M 230_Y 2.115 1.00
257_A 282_C 2.072 1.00
270_M 274_E 1.996 1.00
265_C 278_F 1.996 1.00
213_H 232_S 1.98 1.00
62_A 124_Y 1.946 1.00
9_I 47_A 1.94 1.00
298_S 301_N 1.929 1.00
62_A 89_V 1.916 1.00
49_N 260_A 1.908 1.00
182_H 218_N 1.903 1.00
188_R 224_M 1.89 1.00
74_T 78_Q 1.855 1.00
273_A 277_R 1.852 1.00
261_G 282_C 1.822 1.00
7_V 50_L 1.812 1.00
151_W 154_D 1.807 1.00
262_L 275_S 1.764 1.00
160_P 182_H 1.763 1.00
239_G 273_A 1.729 1.00
283_S 287_L 1.711 1.00
150_A 176_D 1.702 1.00
299_I 303_I 1.691 1.00
201_G 204_D 1.676 1.00
77_N 83_V 1.673 1.00
224_M 240_W 1.67 1.00
48_Q 79_S 1.667 1.00
65_S 70_Q 1.658 1.00
156_A 159_V 1.649 1.00
185_K 259_M 1.644 1.00
50_L 264_S 1.59 1.00
70_Q 74_T 1.562 1.00
13_A 61_S 1.555 1.00
75_Q 78_Q 1.551 1.00
244_I 281_G 1.551 1.00
229_V 280_Q 1.551 1.00
211_W 214_Q 1.551 1.00
150_A 154_D 1.541 1.00
221_V 262_L 1.535 1.00
73_L 77_N 1.529 1.00
204_D 207_K 1.524 1.00
39_P 72_L 1.523 1.00
151_W 155_N 1.507 1.00
181_I 184_L 1.498 1.00
137_V 263_A 1.497 1.00
137_V 160_P 1.491 1.00
254_A 287_L 1.49 1.00
164_D 223_S 1.48 1.00
250_N 291_Y 1.48 1.00
183_T 219_R 1.475 1.00
262_L 279_A 1.465 1.00
73_L 86_C 1.465 1.00
37_F 71_S 1.459 1.00
209_A 232_S 1.453 1.00
133_Q 157_A 1.433 1.00
289_C 301_N 1.433 1.00
137_V 162_F 1.43 1.00
262_L 266_W 1.417 1.00
246_T 288_S 1.415 1.00
160_P 218_N 1.411 1.00
268_D 299_I 1.383 0.99
210_A 214_Q 1.37 0.99
241_S 276_V 1.364 0.99
261_G 278_F 1.364 0.99
244_I 277_R 1.351 0.99
191_A 222_L 1.342 0.99
121_T 124_Y 1.331 0.99
261_G 279_A 1.331 0.99
230_Y 240_W 1.325 0.99
51_A 81_V 1.315 0.99
229_V 276_V 1.314 0.99
65_S 88_I 1.313 0.99
206_A 210_A 1.31 0.99
63_V 69_G 1.31 0.99
224_M 227_D 1.306 0.99
10_I 138_I 1.306 0.99
231_Y 239_G 1.303 0.99
219_R 272_F 1.294 0.99
165_P 186_P 1.294 0.99
206_A 238_S 1.292 0.99
274_E 277_R 1.29 0.99
65_S 91_G 1.279 0.99
219_R 233_D 1.276 0.99
17_V 35_I 1.266 0.99
188_R 227_D 1.264 0.99
231_Y 272_F 1.262 0.99
219_R 262_L 1.259 0.99
160_P 183_T 1.258 0.99
13_A 72_L 1.247 0.99
192_E 198_A 1.246 0.98
15_I 71_S 1.233 0.98
48_Q 52_L 1.226 0.98
265_C 275_S 1.226 0.98
285_M 289_C 1.224 0.98
50_L 260_A 1.217 0.98
280_Q 283_S 1.216 0.98
53_L 261_G 1.211 0.98
129_G 155_N 1.21 0.98
50_L 57_A 1.202 0.98
44_R 76_T 1.199 0.98
74_T 77_N 1.199 0.98
163_V 181_I 1.186 0.98
248_V 287_L 1.173 0.98
75_Q 79_S 1.17 0.97
264_S 268_D 1.167 0.97
175_R 215_H 1.163 0.97
69_G 94_T 1.158 0.97
55_N 267_V 1.153 0.97
185_K 223_S 1.138 0.97
233_D 237_E 1.133 0.97
8_V 60_L 1.123 0.97
47_A 57_A 1.121 0.97
63_V 86_C 1.119 0.96
241_S 280_Q 1.118 0.96
209_A 222_L 1.117 0.96
178_L 181_I 1.114 0.96
211_W 215_H 1.114 0.96
207_K 210_A 1.113 0.96
6_Y 58_W 1.113 0.96
63_V 73_L 1.11 0.96
22_H 33_G 1.11 0.96
140_A 161_V 1.108 0.96
262_L 272_F 1.108 0.96
92_E 119_A 1.106 0.96
246_T 287_L 1.106 0.96
126_A 151_W 1.105 0.96
301_N 304_S 1.105 0.96
39_P 75_Q 1.101 0.96
130_E 133_Q 1.097 0.96
224_M 228_G 1.095 0.96
223_S 258_M 1.094 0.96
285_M 288_S 1.09 0.96
49_N 257_A 1.087 0.96
67_F 70_Q 1.086 0.96
233_D 236_G 1.079 0.95
197_I 207_K 1.072 0.95
56_K 82_Y 1.068 0.95
122_A 151_W 1.067 0.95
197_I 211_W 1.06 0.95
244_I 280_Q 1.054 0.95
71_S 74_T 1.054 0.95
139_V 259_M 1.053 0.95
283_S 286_A 1.048 0.94
22_H 103_N 1.046 0.94
275_S 279_A 1.045 0.94
277_R 281_G 1.038 0.94
185_K 262_L 1.034 0.94
187_N 190_E 1.031 0.94
191_A 208_V 1.031 0.94
9_I 43_G 1.023 0.93
66_D 94_T 1.019 0.93
140_A 153_L 1.017 0.93
132_I 159_V 1.017 0.93
183_T 262_L 1.017 0.93
14_N 95_S 1.007 0.93
219_R 266_W 1.002 0.92
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
2fv7A20.97441000.178Contact Map0.815
2rbcA10.96811000.182Contact Map0.777
1rkdA10.97761000.183Contact Map0.836
3kzhA20.99361000.184Contact Map0.732
3ry7A10.95531000.195Contact Map0.771
1vm7A20.95531000.199Contact Map0.834
2nwhA10.9841000.21Contact Map0.689
3ljsA20.96171000.224Contact Map0.75
4du5A40.94251000.224Contact Map0.81
3vasA20.97121000.227Contact Map0.743
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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