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OPENSEQ.org
DDLA - D-alanine--D-alanine ligase A
UniProt: P0A6J8 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG10213
Length: 364 (341)
Sequences: 6340
Seq/Len: 18.59
DDLA
Paralog alert: 0.89 [within 20: 0.61] - ratio of genomes with paralogs
Cluster includes: ACCC DDLA DDLB GSHB PUR2 PURK PURT RIMK
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
157_P 222_Q 4.54 1.00
284_A 288_Q 4.158 1.00
5_R 37_D 4.112 1.00
200_S 203_Q 3.926 1.00
275_A 278_I 3.699 1.00
176_S 201_E 3.587 1.00
276_P 280_D 3.246 1.00
150_D 292_T 3.14 1.00
172_A 205_A 2.964 1.00
118_G 122_V 2.741 1.00
287_V 291_Q 2.699 1.00
180_L 201_E 2.567 1.00
159_I 177_K 2.464 1.00
7_G 41_L 2.448 1.00
281_K 284_A 2.418 1.00
154_N 310_E 2.404 1.00
227_R 246_E 2.324 1.00
148_L 289_A 2.246 1.00
173_E 177_K 2.242 1.00
4_L 100_D 2.228 1.00
148_L 313_I 2.193 1.00
199_T 203_Q 2.184 1.00
338_G 341_D 2.098 1.00
154_N 285_I 2.089 1.00
153_L 313_I 2.068 1.00
201_E 205_A 2.043 1.00
133_L 143_V 1.985 1.00
175_E 180_L 1.984 1.00
306_T 310_E 1.981 1.00
182_L 220_V 1.976 1.00
157_P 177_K 1.958 1.00
280_D 284_A 1.941 1.00
36_F 344_T 1.93 1.00
278_I 281_K 1.922 1.00
288_Q 291_Q 1.918 1.00
280_D 283_R 1.9 1.00
205_A 209_D 1.89 1.00
281_K 310_E 1.879 1.00
118_G 136_A 1.863 1.00
231_C 282_I 1.854 1.00
159_I 174_V 1.85 1.00
145_K 221_E 1.808 1.00
146_R 150_D 1.804 1.00
305_L 311_V 1.798 1.00
6_V 36_F 1.793 1.00
172_A 201_E 1.788 1.00
5_R 39_V 1.787 1.00
233_V 286_A 1.735 1.00
278_I 305_L 1.705 1.00
44_D 77_A 1.689 1.00
198_V 207_A 1.666 1.00
344_T 348_E 1.645 1.00
149_R 157_P 1.645 1.00
136_A 143_V 1.644 1.00
121_R 132_V 1.627 1.00
120_L 125_L 1.608 1.00
142_D 146_R 1.601 1.00
101_V 350_A 1.598 1.00
199_T 225_K 1.587 1.00
229_I 305_L 1.583 1.00
210_L 213_E 1.583 1.00
121_R 127_F 1.564 1.00
26_N 339_Y 1.561 1.00
270_V 334_A 1.533 1.00
7_G 99_V 1.529 1.00
6_V 128_V 1.482 1.00
174_V 177_K 1.481 1.00
137_A 147_L 1.479 1.00
147_L 292_T 1.474 1.00
183_F 197_K 1.461 1.00
282_I 311_V 1.456 1.00
196_S 210_L 1.452 1.00
274_I 278_I 1.452 1.00
184_V 207_A 1.431 1.00
39_V 99_V 1.42 1.00
8_I 28_V 1.418 1.00
184_V 204_Y 1.418 1.00
206_I 210_L 1.415 1.00
143_V 146_R 1.412 1.00
131_D 134_A 1.405 0.99
144_T 148_L 1.403 0.99
163_R 166_R 1.401 0.99
137_A 143_V 1.396 0.99
320_P 342_L 1.386 0.99
6_V 101_V 1.382 0.99
145_K 155_I 1.382 0.99
212_F 216_H 1.371 0.99
231_C 286_A 1.366 0.99
187_A 217_K 1.358 0.99
94_Q 102_I 1.345 0.99
118_G 132_V 1.321 0.99
301_V 316_I 1.319 0.99
269_V 273_A 1.313 0.99
149_R 155_I 1.312 0.99
208_V 218_V 1.31 0.99
175_E 182_L 1.302 0.99
225_K 307_P 1.278 0.99
298_M 319_L 1.275 0.99
134_A 294_G 1.275 0.99
277_E 309_N 1.274 0.99
119_M 123_A 1.271 0.99
76_L 97_P 1.267 0.99
290_Y 299_A 1.266 0.99
28_V 38_V 1.256 0.99
138_C 316_I 1.241 0.98
279_N 283_R 1.234 0.98
33_K 340_T 1.234 0.98
231_C 283_R 1.221 0.98
121_R 131_D 1.213 0.98
22_Q 324_N 1.212 0.98
290_Y 301_V 1.197 0.98
171_F 208_V 1.193 0.98
131_D 296_A 1.19 0.98
188_N 215_D 1.186 0.98
184_V 220_V 1.186 0.98
29_D 33_K 1.184 0.98
235_G 349_L 1.182 0.98
286_A 303_V 1.179 0.98
245_G 331_L 1.17 0.97
180_L 204_Y 1.164 0.97
116_L 136_A 1.162 0.97
275_A 309_N 1.156 0.97
277_E 281_K 1.154 0.97
289_A 313_I 1.152 0.97
244_C 279_N 1.15 0.97
234_L 349_L 1.147 0.97
153_L 285_I 1.144 0.97
138_C 293_L 1.137 0.97
197_K 223_G 1.127 0.97
135_S 318_T 1.119 0.96
109_T 113_D 1.111 0.96
238_N 291_Q 1.102 0.96
231_C 244_C 1.101 0.96
257_D 261_I 1.098 0.96
208_V 212_F 1.094 0.96
284_A 287_V 1.086 0.96
232_A 331_L 1.086 0.96
172_A 176_S 1.084 0.96
281_K 285_I 1.081 0.95
229_I 282_I 1.074 0.95
27_I 103_F 1.073 0.95
31_I 38_V 1.071 0.95
77_A 81_G 1.07 0.95
248_V 267_K 1.066 0.95
32_D 35_R 1.062 0.95
277_E 280_D 1.06 0.95
348_E 353_R 1.056 0.95
95_P 123_A 1.055 0.95
6_V 347_I 1.053 0.95
165_N 173_E 1.048 0.94
285_I 303_V 1.047 0.94
162_T 166_R 1.046 0.94
4_L 36_F 1.046 0.94
241_A 287_V 1.045 0.94
26_N 324_N 1.044 0.94
276_P 279_N 1.041 0.94
162_T 217_K 1.034 0.94
143_V 147_L 1.033 0.94
156_A 312_V 1.032 0.94
178_L 182_L 1.03 0.94
133_L 137_A 1.024 0.93
119_M 122_V 1.019 0.93
246_E 273_A 1.018 0.93
154_N 281_K 1.013 0.93
104_P 128_V 1.007 0.93
180_L 200_S 1.005 0.93
28_V 32_D 1.003 0.93
156_A 224_I 1.002 0.92
8_I 38_V 1.002 0.92
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
3i12A411000.185Contact Map0.725
3e5nA111000.199Contact Map0.654
1ehiA20.9781000.202Contact Map0.75
3tqtA20.98631000.207Contact Map0.765
2i87A20.9671000.208Contact Map0.75
2fb9A10.88191000.215Contact Map0.697
3lwbA20.97251000.217Contact Map0.69
4fu0A20.9671000.218Contact Map0.739
3k3pA20.94781000.228Contact Map0.735
1e4eA10.92861000.229Contact Map0.625
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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