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OPENSEQ.org
GSHB - Glutathione synthetase
UniProt: P04425 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG10419
Length: 316 (312)
Sequences: 1680
Seq/Len: 5.38
GSHB
Paralog alert: 0.60 [within 20: 0.02] - ratio of genomes with paralogs
Cluster includes: ACCC DDLA DDLB GSHB PUR2 PURK PURT RIMK
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
27_E 300_S 3.776 1.00
137_E 199_N 3.74 1.00
31_R 307_D 3.374 1.00
3_K 34_E 3.271 1.00
20_S 290_I 3.152 1.00
253_K 257_Q 2.77 1.00
211_V 274_I 2.506 1.00
261_T 264_E 2.44 1.00
106_E 115_K 2.374 1.00
214_V 305_L 2.332 1.00
33_Y 310_E 2.332 1.00
250_S 253_K 2.312 1.00
215_D 308_A 2.239 1.00
62_N 65_E 2.2 1.00
28_A 306_M 2.191 1.00
36_H 80_L 2.189 1.00
156_D 172_R 2.184 1.00
217_E 304_M 2.176 1.00
214_V 219_V 2.133 1.00
151_W 157_I 2.059 1.00
27_E 303_G 2.04 1.00
27_E 30_R 2.032 1.00
28_A 35_L 1.991 1.00
46_I 153_K 1.926 1.00
304_M 307_D 1.896 1.00
5_G 80_L 1.89 1.00
106_E 116_P 1.889 1.00
25_L 57_L 1.867 1.00
267_L 270_V 1.807 1.00
54_T 75_L 1.742 1.00
300_S 303_G 1.687 1.00
25_L 37_Y 1.675 1.00
85_M 98_A 1.647 0.99
31_R 303_G 1.646 0.99
147_L 197_A 1.624 0.99
217_E 256_R 1.617 0.99
213_V 256_R 1.549 0.99
261_T 265_K 1.538 0.99
33_Y 306_M 1.526 0.99
65_E 68_S 1.502 0.99
216_G 260_P 1.5 0.99
82_V 309_I 1.499 0.99
254_I 279_L 1.497 0.99
159_L 197_A 1.482 0.99
5_G 38_M 1.473 0.99
249_E 256_R 1.471 0.99
275_I 280_T 1.47 0.98
27_E 31_R 1.457 0.98
236_L 242_G 1.456 0.98
56_T 70_V 1.453 0.98
201_L 278_R 1.445 0.98
303_G 307_D 1.433 0.98
134_L 257_Q 1.407 0.98
258_I 272_L 1.402 0.98
308_A 312_R 1.4 0.98
30_R 303_G 1.396 0.98
139_L 150_F 1.39 0.98
60_K 65_E 1.388 0.98
12_A 55_R 1.386 0.98
147_L 157_I 1.373 0.97
27_E 307_D 1.37 0.97
129_A 135_T 1.363 0.97
169_S 182_V 1.359 0.97
8_M 14_I 1.346 0.97
149_A 153_K 1.333 0.97
218_P 256_R 1.333 0.97
207_G 224_A 1.328 0.97
217_E 252_W 1.328 0.97
219_V 304_M 1.319 0.96
252_W 256_R 1.312 0.96
249_E 252_W 1.312 0.96
122_C 267_L 1.308 0.96
76_P 79_D 1.307 0.96
20_S 24_M 1.292 0.96
211_V 247_L 1.289 0.96
111_L 309_I 1.287 0.96
14_I 39_E 1.272 0.95
268_I 308_A 1.272 0.95
215_D 311_A 1.271 0.95
217_E 249_E 1.269 0.95
36_H 54_T 1.255 0.95
58_N 68_S 1.252 0.95
106_E 109_G 1.249 0.95
99_T 120_R 1.246 0.95
31_R 310_E 1.234 0.94
213_V 259_G 1.233 0.94
148_K 176_G 1.231 0.94
224_A 243_E 1.222 0.94
102_L 112_I 1.22 0.94
2_I 33_Y 1.214 0.94
133_D 257_Q 1.208 0.93
225_R 235_N 1.206 0.93
143_N 146_Q 1.204 0.93
23_A 294_E 1.202 0.93
167_G 235_N 1.197 0.93
273_D 281_E 1.193 0.93
4_L 306_M 1.19 0.93
50_A 101_I 1.185 0.93
158_I 232_T 1.18 0.92
105_A 109_G 1.178 0.92
16_I 22_F 1.174 0.92
203_A 206_D 1.171 0.92
146_Q 149_A 1.162 0.92
122_C 284_V 1.162 0.92
36_H 75_L 1.161 0.92
225_R 240_G 1.157 0.91
25_L 29_Q 1.155 0.91
152_E 176_G 1.149 0.91
223_L 242_G 1.147 0.91
245_R 251_D 1.141 0.91
209_K 245_R 1.139 0.90
226_I 243_E 1.13 0.90
215_D 312_R 1.129 0.90
51_R 74_D 1.124 0.90
53_H 56_T 1.115 0.89
7_V 287_P 1.115 0.89
299_V 304_M 1.115 0.89
215_D 268_I 1.104 0.89
104_R 130_W 1.103 0.89
208_D 225_R 1.091 0.88
188_T 195_C 1.089 0.88
148_K 181_G 1.088 0.88
56_T 264_E 1.085 0.87
155_S 175_E 1.077 0.87
213_V 218_P 1.073 0.87
87_K 97_Y 1.07 0.86
156_D 174_K 1.065 0.86
225_R 239_G 1.062 0.86
218_P 259_G 1.058 0.86
4_L 33_Y 1.057 0.86
248_T 251_D 1.054 0.85
36_H 56_T 1.053 0.85
38_M 52_A 1.053 0.85
294_E 302_T 1.051 0.85
137_E 154_H 1.046 0.85
250_S 278_R 1.044 0.85
269_F 305_L 1.043 0.84
212_L 269_F 1.038 0.84
125_K 283_N 1.032 0.84
156_D 287_P 1.03 0.83
293_I 299_V 1.026 0.83
56_T 72_E 1.024 0.83
200_Y 205_K 1.024 0.83
27_E 306_M 1.019 0.83
23_A 302_T 1.015 0.82
151_W 180_L 1.012 0.82
145_A 149_A 1.007 0.82
170_I 234_G 1.006 0.81
56_T 73_Q 1.006 0.81
207_G 226_I 1.003 0.81
134_L 253_K 1.002 0.81
6_I 21_S 1.001 0.81
189_E 193_R 1.001 0.81
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
1gsaA111000.333Contact Map0.754
4iwxA10.89871000.465Contact Map0.532
3vpbA40.84811000.469Contact Map0.74
3vpdA20.85131000.506Contact Map0.711
2p0aA20.89561000.544Contact Map0.375
1pk8A80.91771000.564Contact Map0.471
3t7aA10.85761000.581Contact Map0.531
2q7dA20.82911000.588Contact Map0.588
1i7nA20.90821000.589Contact Map0.415
3r5xA40.86081000.59Contact Map0.495
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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