OPENSEQ.org - ULAC - ECOLI GREMLIN

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ULAC - Ascorbate-specific phosphotransferase enzyme IIA component

UniProt:	P69820	Sequence MKLRDSLAENKSIRLQAEAETWQEAVKIGVDLLVAADVVEPRYYQAILDGVEQFGPYFVIAPGLAMPHGRPEEGVKKTGFSLVTLKKPLEFNHDDNDPVDILITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA	Download Alignment We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene:	EG12495
Length:	154 (147)
Sequences:	3513
Seq/Len:	23.90

ULAC
Paralog alert: 0.74 [within 20: 0.20] - ratio of genomes with paralogs
Cluster includes: PTFA PTKA PTMA PTSN SGCA ULAC

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Scaled Score	Prob
100_D	135_R	3.667	1.00
10_N	141_Q	2.91	1.00
31_D	35_A	2.435	1.00
21_T	24_E	2.353	1.00
139_T	142_E	2.336	1.00
40_E	76_K	2.271	1.00
132_D	136_A	2.233	1.00
27_K	44_Y	2.118	1.00
29_G	104_T	2.091	1.00
30_V	44_Y	2.024	1.00
18_E	86_K	2.022	1.00
16_Q	84_T	2.003	1.00
85_L	102_L	1.977	1.00
23_Q	52_E	1.961	1.00
19_A	25_A	1.955	1.00
45_Q	49_D	1.942	1.00
47_I	104_T	1.927	1.00
79_G	106_A	1.885	1.00
88_P	100_D	1.875	1.00
16_Q	86_K	1.867	1.00
38_V	77_K	1.84	1.00
19_A	28_I	1.837	1.00
14_R	32_L	1.833	1.00
34_V	39_V	1.815	1.00
29_G	83_V	1.737	1.00
85_L	88_P	1.721	1.00
25_A	102_L	1.714	1.00
138_R	142_E	1.711	1.00
75_V	106_A	1.701	1.00
17_A	83_V	1.569	1.00
51_V	58_F	1.551	1.00
20_E	24_E	1.547	1.00
12_S	36_A	1.528	1.00
141_Q	145_D	1.515	1.00
11_K	36_A	1.454	1.00
43_Y	106_A	1.445	1.00
42_R	74_G	1.383	0.99
14_R	28_I	1.376	0.99
32_L	35_A	1.373	0.99
51_V	56_P	1.341	0.99
38_V	79_G	1.339	0.99
131_F	135_R	1.331	0.99
85_L	100_D	1.319	0.99
78_T	108_V	1.315	0.99
26_V	47_I	1.314	0.99
49_D	52_E	1.302	0.99
46_A	49_D	1.295	0.99
137_C	142_E	1.291	0.99
19_A	85_L	1.282	0.99
10_N	144_L	1.275	0.99
15_L	84_T	1.233	0.98
30_V	104_T	1.225	0.98
99_V	102_L	1.205	0.98
84_T	101_I	1.204	0.98
62_P	131_F	1.199	0.98
23_Q	48_L	1.198	0.98
49_D	53_Q	1.194	0.98
27_K	48_L	1.19	0.98
137_C	143_V	1.189	0.98
137_C	146_L	1.175	0.98
13_I	143_V	1.169	0.97
23_Q	27_K	1.151	0.97
30_V	47_I	1.114	0.96
119_M	122_V	1.103	0.96
63_G	100_D	1.094	0.96
10_N	140_E	1.086	0.96
24_E	27_K	1.084	0.96
82_L	101_I	1.07	0.95
90_E	98_P	1.067	0.95
17_A	28_I	1.066	0.95
83_V	104_T	1.06	0.95
33_L	106_A	1.05	0.94
32_L	36_A	1.034	0.94
78_T	107_A	1.03	0.94
144_L	148_D	1.018	0.93

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling

HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:

Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	P(%)	HHΔ		Acc
3bjvA	1	0.9805	100	0.108	Contact Map	0.803
2oq3A	1	0.961	100	0.112	Contact Map	0.677
1a6jA	2	0.974	100	0.114	Contact Map	0.754
2oqtA	4	0.9805	100	0.115	Contact Map	0.809
1a3aA	4	0.9481	100	0.117	Contact Map	0.78
2a0jA	1	0.9481	100	0.118	Contact Map	0.678
3oxpA	2	0.961	100	0.119	Contact Map	0.781
3urrA	2	0.961	100	0.127	Contact Map	0.763
3lf6A	2	0.974	100	0.129	Contact Map	0.707
3t43A	2	0.8636	100	0.192	Contact Map	0.773

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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