OPENSEQ.org - PTSN - ECOLI GREMLIN

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PTSN - Nitrogen regulatory protein

UniProt:	P69829	Sequence MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLSLPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQPVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTPDEA	Download Alignment We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene:	EG11682
Length:	163 (150)
Sequences:	3433
Seq/Len:	22.89

PTSN
Paralog alert: 0.75 [within 20: 0.20] - ratio of genomes with paralogs
Cluster includes: PTFA PTKA PTMA PTSN SGCA ULAC

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Scaled Score	Prob
105_D	141_R	3.577	1.00
16_R	147_E	2.93	1.00
145_S	148_E	2.458	1.00
37_E	41_K	2.363	1.00
27_S	30_R	2.327	1.00
138_R	142_A	2.28	1.00
33_E	51_F	2.223	1.00
24_H	91_E	2.189	1.00
22_R	89_Q	2.163	1.00
35_I	109_A	2.07	1.00
90_L	107_L	2.026	1.00
54_I	109_A	2.008	1.00
25_C	31_A	1.987	1.00
20_R	38_L	1.931	1.00
36_S	51_F	1.902	1.00
22_R	91_E	1.836	1.00
25_C	34_I	1.834	1.00
84_V	111_L	1.822	1.00
90_L	93_P	1.781	1.00
93_P	105_D	1.773	1.00
144_Q	148_E	1.765	1.00
49_V	79_D	1.749	1.00
31_A	107_L	1.743	1.00
35_I	88_V	1.739	1.00
52_E	56_T	1.666	1.00
26_Q	30_R	1.643	1.00
50_V	111_L	1.621	1.00
23_V	88_V	1.611	1.00
32_L	54_I	1.599	1.00
147_E	151_Q	1.59	1.00
44_S	82_R	1.558	1.00
58_E	63_T	1.542	1.00
80_T	111_L	1.507	1.00
17_E	42_Q	1.493	1.00
46_P	49_V	1.481	1.00
20_R	34_I	1.452	1.00
56_T	59_K	1.443	1.00
38_L	41_K	1.413	1.00
18_C	42_Q	1.39	0.99
46_P	81_L	1.372	0.99
83_A	113_P	1.366	0.99
16_R	150_Y	1.365	0.99
36_S	54_I	1.349	0.99
104_V	107_L	1.335	0.99
29_K	58_E	1.33	0.99
143_A	148_E	1.321	0.99
137_C	141_R	1.312	0.99
37_E	47_P	1.31	0.99
57_R	63_T	1.283	0.99
90_L	105_D	1.268	0.99
32_L	51_F	1.266	0.99
143_A	149_L	1.229	0.98
25_C	90_L	1.225	0.98
68_G	105_D	1.202	0.98
21_S	89_Q	1.187	0.98
29_K	55_L	1.184	0.98
36_S	109_A	1.184	0.98
29_K	33_E	1.174	0.98
40_A	45_L	1.167	0.97
77_E	114_A	1.159	0.97
48_Q	52_E	1.143	0.97
40_A	47_P	1.132	0.97
87_F	106_L	1.126	0.97
95_A	103_P	1.113	0.96
143_A	152_I	1.11	0.96
56_T	60_M	1.097	0.96
67_N	137_C	1.09	0.96
120_H	124_L	1.078	0.95
44_S	84_V	1.071	0.95
30_R	33_E	1.068	0.95
19_T	149_L	1.061	0.95
38_L	42_Q	1.06	0.95
53_A	56_T	1.059	0.95
88_V	109_A	1.057	0.95
16_R	146_D	1.051	0.94
39_A	84_V	1.048	0.94
89_Q	106_L	1.048	0.94
39_A	86_V	1.042	0.94
15_N	18_C	1.012	0.93
150_Y	154_T	1.007	0.93
23_V	34_I	1.003	0.93

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling

HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:

Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	P(%)	HHΔ		Acc
1a6jA	2	0.9877	100	0.097	Contact Map	0.776
2a0jA	1	0.9141	100	0.182	Contact Map	0.702
3urrA	2	0.9325	100	0.182	Contact Map	0.791
1a3aA	4	0.8957	100	0.188	Contact Map	0.775
3oxpA	2	0.9018	100	0.205	Contact Map	0.751
2oq3A	1	0.908	100	0.209	Contact Map	0.641
3bjvA	1	0.9264	100	0.222	Contact Map	0.772
3lf6A	2	0.9387	100	0.226	Contact Map	0.689
2oqtA	4	0.9325	100	0.227	Contact Map	0.801
3t43A	2	0.8221	100	0.268	Contact Map	0.74

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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