OPENSEQ.org - PTKA - ECOLI GREMLIN

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PTKA - Galactitol-specific phosphotransferase enzyme IIA component

UniProt:	P69828	Sequence MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALIAREAEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALIVENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE	Download Alignment We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene:	EG12414
Length:	150 (141)
Sequences:	3360
Seq/Len:	23.83

PTKA
Paralog alert: 0.73 [within 20: 0.19] - ratio of genomes with paralogs
Cluster includes: PTFA PTKA PTMA PTSN SGCA ULAC

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Scaled Score	Prob
96_S	130_I	3.704	1.00
24_N	28_A	2.465	1.00
14_D	17_E	2.39	1.00
20_T	37_P	2.339	1.00
127_E	131_T	2.265	1.00
33_H	69_K	2.185	1.00
11_S	79_T	2.106	1.00
78_P	98_V	2.091	1.00
22_I	100_A	2.064	1.00
23_G	37_P	2.061	1.00
9_G	77_R	2.045	1.00
40_L	100_A	2.038	1.00
12_F	18_V	2.027	1.00
9_G	79_T	1.988	1.00
72_A	102_I	1.942	1.00
27_L	32_V	1.914	1.00
7_R	25_E	1.86	1.00
81_K	96_S	1.859	1.00
38_Q	42_A	1.852	1.00
31_V	70_S	1.844	1.00
12_F	21_H	1.776	1.00
22_I	76_L	1.764	1.00
35_T	67_H	1.73	1.00
68_A	102_I	1.687	1.00
133_P	136_Q	1.686	1.00
18_V	98_V	1.647	1.00
16_S	45_A	1.643	1.00
78_P	81_K	1.605	1.00
13_V	17_E	1.595	1.00
10_I	76_L	1.586	1.00
36_W	102_I	1.541	1.00
19_L	40_L	1.523	1.00
44_E	51_I	1.486	1.00
71_S	104_E	1.459	1.00
5_F	29_K	1.422	1.00
7_R	21_H	1.42	1.00
25_E	28_A	1.38	0.99
44_E	49_T	1.376	0.99
31_V	72_A	1.365	0.99
42_A	45_A	1.359	0.99
126_V	130_I	1.35	0.99
16_S	41_I	1.345	0.99
23_G	40_L	1.318	0.99
19_L	37_P	1.317	0.99
132_L	136_Q	1.317	0.99
95_V	98_V	1.312	0.99
16_S	20_T	1.277	0.99
78_P	96_S	1.261	0.99
12_F	78_P	1.244	0.98
8_S	77_R	1.22	0.98
57_A	96_S	1.22	0.98
56_H	126_V	1.209	0.98
23_G	100_A	1.196	0.98
6_V	137_L	1.183	0.98
42_A	46_E	1.174	0.98
17_E	20_T	1.158	0.97
20_T	41_I	1.119	0.96
132_L	137_L	1.111	0.96
25_E	29_K	1.107	0.96
75_L	97_L	1.098	0.96
27_L	34_D	1.087	0.96
73_I	112_L	1.087	0.96
83_H	94_A	1.086	0.96
10_I	21_H	1.077	0.95
77_R	97_L	1.071	0.95
43_R	49_T	1.069	0.95
4_L	29_K	1.066	0.95
132_L	140_Y	1.063	0.95
71_S	103_V	1.043	0.94
39_A	42_A	1.042	0.94
138_K	142_T	1.033	0.94
39_A	63_C	1.033	0.94
114_R	117_F	1.031	0.94
109_Q	113_L	1.023	0.93
75_L	137_L	1.014	0.93
26_M	74_Y	1.011	0.93

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling

HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:

Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	P(%)	HHΔ		Acc
1a6jA	2	0.96	100	0.133	Contact Map	0.739
3lf6A	2	0.98	100	0.137	Contact Map	0.752
1a3aA	4	0.92	100	0.152	Contact Map	0.818
2oq3A	1	0.9267	100	0.154	Contact Map	0.725
3oxpA	2	0.92	100	0.154	Contact Map	0.787
3bjvA	1	0.96	100	0.174	Contact Map	0.852
2a0jA	1	0.92	100	0.174	Contact Map	0.657
3urrA	2	0.9333	100	0.175	Contact Map	0.728
2oqtA	4	0.9467	100	0.175	Contact Map	0.853
3t43A	2	0.9133	99.9	0.197	Contact Map	0.788

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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