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OPENSEQ.org

GSTB - Glutathione S-transferase GstB
UniProt: P0ACA7 Sequence
Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG13481
Length: 208 (202)
Sequences: 11328
Seq/Len: 56.08

GSTB
Paralog alert: 0.77 [within 20: 0.33] - ratio of genomes with paralogs
Cluster includes: GSTA GSTB SSPA YFCF YFCG YIBF YNCG
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
3_T 30_I 3.366 1.00
73_R 96_E 3.277 1.00
188_R 192_Q 3.252 1.00
22_E 196_R 3.184 1.00
59_D 62_D 2.826 1.00
77_A 92_R 2.817 1.00
3_T 28_E 2.805 1.00
20_L 27_Y 2.594 1.00
21_E 201_K 2.583 1.00
145_D 185_N 2.577 1.00
21_E 27_Y 2.54 1.00
45_L 51_G 2.505 1.00
152_K 192_Q 2.466 1.00
6_G 13_V 2.428 1.00
63_L 90_A 2.324 1.00
149_A 185_N 2.184 1.00
142_A 183_R 2.16 1.00
88_S 91_R 2.061 1.00
57_R 64_I 1.911 1.00
152_K 157_D 1.88 1.00
3_T 57_R 1.812 1.00
47_M 55_L 1.773 1.00
77_A 96_E 1.772 1.00
33_G 36_F 1.766 1.00
6_G 29_Q 1.765 1.00
187_Q 191_Q 1.745 1.00
42_A 46_A 1.717 1.00
5_W 57_R 1.705 1.00
44_F 55_L 1.702 1.00
18_L 199_V 1.701 1.00
127_Q 131_D 1.684 1.00
22_E 198_A 1.629 1.00
138_D 182_P 1.627 1.00
185_N 188_R 1.624 1.00
154_F 166_I 1.604 1.00
94_E 97_K 1.567 1.00
5_W 32_A 1.564 1.00
19_T 72_V 1.545 1.00
142_A 146_A 1.533 1.00
23_L 76_A 1.531 1.00
57_R 62_D 1.523 1.00
159_F 164_I 1.519 1.00
191_Q 195_E 1.515 1.00
86_I 95_A 1.46 1.00
131_D 135_K 1.456 1.00
195_E 200_R 1.455 1.00
47_M 57_R 1.455 1.00
145_D 184_P 1.446 1.00
98_W 147_E 1.439 1.00
58_D 74_Y 1.424 1.00
2_I 56_L 1.413 1.00
155_S 160_G 1.389 0.99
25_L 79_Y 1.383 0.99
43_D 46_A 1.375 0.99
147_E 151_V 1.353 0.99
167_A 193_L 1.352 0.99
19_T 84_L 1.31 0.99
4_L 20_L 1.302 0.99
65_L 93_A 1.302 0.99
2_I 25_L 1.283 0.99
16_V 72_V 1.278 0.99
159_F 196_R 1.27 0.99
44_F 47_M 1.264 0.99
19_T 23_L 1.257 0.99
153_W 193_L 1.25 0.99
38_I 41_D 1.234 0.98
134_C 138_D 1.222 0.98
52_L 67_E 1.216 0.98
66_W 97_K 1.212 0.98
58_D 78_Q 1.201 0.98
85_W 95_A 1.198 0.98
84_L 164_I 1.187 0.98
40_H 51_G 1.184 0.98
73_R 100_D 1.169 0.97
78_Q 89_P 1.148 0.97
132_A 136_E 1.148 0.97
22_E 164_I 1.142 0.97
100_D 103_N 1.141 0.97
145_D 149_A 1.137 0.97
72_V 161_V 1.136 0.97
22_E 159_F 1.134 0.97
197_P 201_K 1.123 0.97
91_R 158_E 1.115 0.96
142_A 182_P 1.114 0.96
147_E 150_K 1.108 0.96
58_D 63_L 1.099 0.96
136_E 139_A 1.093 0.96
59_D 63_L 1.091 0.96
89_P 93_A 1.089 0.96
90_A 94_E 1.088 0.96
58_D 79_Y 1.086 0.96
190_Y 194_T 1.063 0.95
167_A 194_T 1.053 0.95
146_A 149_A 1.052 0.95
200_R 204_M 1.043 0.94
16_V 71_I 1.042 0.94
15_K 165_A 1.035 0.94
102_A 166_I 1.03 0.94
122_P 125_R 1.028 0.94
135_K 139_A 1.025 0.93
153_W 192_Q 1.023 0.93
179_T 182_P 1.021 0.93
181_T 187_Q 1.012 0.93
191_Q 194_T 1.009 0.93
128_A 131_D 1.004 0.93
17_L 21_E 1 0.92
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
4kdxA20.99041000.105Contact Map0.757
4ielA20.99041000.108Contact Map0.738
4kh7A211000.113Contact Map0.748
2v6kA20.99041000.133Contact Map0.727
4gf0A20.98561000.139Contact Map0.731
3einA10.96631000.144Contact Map0.634
3nivA40.99041000.146Contact Map0.735
2x64A60.9761000.152Contact Map0.804
1e6bA10.98081000.152Contact Map0.641
2imiA20.96631000.153Contact Map0.721

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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