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OPENSEQ.org

SSPA - Stringent starvation protein A
UniProt: P0ACA3 Sequence
Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG10977
Length: 212 (196)
Sequences: 11147
Seq/Len: 56.87

SSPA
Paralog alert: 0.77 [within 20: 0.32] - ratio of genomes with paralogs
Cluster includes: GSTA GSTB SSPA YFCF YFCG YIBF YNCG
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
12_T 39_E 3.496 1.00
77_E 100_R 3.3 1.00
185_G 189_R 3.296 1.00
31_E 193_R 3.254 1.00
12_T 37_E 2.855 1.00
81_E 96_R 2.84 1.00
30_A 198_A 2.662 1.00
29_L 36_F 2.611 1.00
15_S 22_S 2.563 1.00
140_A 182_E 2.545 1.00
51_I 57_Q 2.476 1.00
30_A 36_F 2.466 1.00
147_P 189_R 2.457 1.00
67_L 94_V 2.357 1.00
137_L 180_A 2.196 1.00
144_G 182_E 2.126 1.00
92_Y 95_A 2.031 1.00
147_P 152_D 1.884 1.00
12_T 63_V 1.848 1.00
53_L 61_T 1.822 1.00
63_V 68_T 1.817 1.00
184_K 188_T 1.763 1.00
15_S 38_I 1.757 1.00
81_E 100_R 1.756 1.00
27_I 196_F 1.702 1.00
14_F 63_V 1.699 1.00
48_Q 52_D 1.699 1.00
50_L 61_T 1.677 1.00
31_E 195_S 1.658 1.00
133_R 179_G 1.657 1.00
98_E 101_L 1.626 1.00
174_E 178_P 1.602 1.00
126_D 130_K 1.597 1.00
182_E 185_G 1.593 1.00
149_F 161_L 1.587 1.00
28_V 76_M 1.577 1.00
154_F 159_C 1.561 1.00
14_F 41_V 1.559 1.00
188_T 192_E 1.526 1.00
90_P 99_S 1.501 1.00
137_L 141_P 1.494 1.00
140_A 181_K 1.491 1.00
32_K 80_D 1.483 1.00
11_M 62_L 1.48 1.00
162_A 190_V 1.479 1.00
63_V 66_E 1.478 1.00
102_Y 142_V 1.463 1.00
49_D 52_D 1.452 1.00
192_E 197_L 1.45 1.00
53_L 63_V 1.435 1.00
34_V 83_F 1.418 1.00
150_L 155_S 1.396 0.99
64_D 78_Y 1.35 0.99
142_V 146_K 1.349 0.99
13_L 29_L 1.333 0.99
69_L 97_G 1.292 0.99
25_V 76_M 1.283 0.99
50_L 53_L 1.274 0.99
28_V 32_K 1.232 0.98
154_F 193_R 1.231 0.98
88_L 159_C 1.218 0.98
11_M 34_V 1.215 0.98
64_D 82_R 1.212 0.98
28_V 88_L 1.198 0.98
148_Y 190_V 1.198 0.98
58_S 71_E 1.189 0.98
77_E 104_H 1.188 0.98
95_A 153_E 1.181 0.98
70_W 101_L 1.177 0.98
89_M 99_S 1.174 0.98
46_P 57_Q 1.17 0.97
129_R 133_R 1.163 0.97
178_P 184_K 1.155 0.97
106_I 161_L 1.151 0.97
31_E 154_F 1.147 0.97
82_R 93_P 1.135 0.97
104_H 107_E 1.129 0.97
140_A 144_G 1.125 0.97
76_M 156_L 1.125 0.97
31_E 159_C 1.115 0.96
194_D 198_A 1.115 0.96
94_V 98_E 1.113 0.96
137_L 179_G 1.107 0.96
197_L 201_T 1.099 0.96
93_P 97_G 1.098 0.96
24_Q 72_S 1.076 0.95
142_V 145_Q 1.074 0.95
11_M 83_F 1.067 0.95
127_A 131_Q 1.062 0.95
24_Q 160_Y 1.056 0.95
42_E 45_N 1.05 0.94
148_Y 189_R 1.045 0.94
162_A 191_F 1.044 0.94
131_Q 134_E 1.044 0.94
187_M 191_F 1.031 0.94
130_K 134_E 1.03 0.94
188_T 191_F 1.026 0.94
99_S 155_S 1.025 0.93
103_M 160_Y 1.022 0.93
25_V 75_I 1.021 0.93
133_R 137_L 1.007 0.93
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
1yy7A211000.197Contact Map0.795
4hojA10.96231000.212Contact Map0.729
4dejA120.98111000.221Contact Map0.806
3lykA20.98111000.226Contact Map0.782
3lypA20.98111000.238Contact Map0.775
4mdcA40.99061000.25Contact Map0.81
2vo4A20.9671000.255Contact Map0.817
4kh7A20.95751000.257Contact Map0.756
1e6bA10.98111000.258Contact Map0.638
2imiA20.95281000.259Contact Map0.72

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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