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OPENSEQ.org
EPMA - Elongation factor P--(R)-beta-lysine ligase
UniProt: P0A8N7 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG11211
Length: 325 (310)
Sequences: 4869
Seq/Len: 15.71
EPMA
Paralog alert: 0.93 [within 20: 0.03] - ratio of genomes with paralogs
Cluster includes: EPMA SYD SYK1 SYK2 SYN
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
33_R 133_D 5.127 1.00
29_F 133_D 4.013 1.00
28_R 32_D 3.757 1.00
31_A 93_F 3.593 1.00
24_A 28_R 3.391 1.00
24_A 31_A 3.241 1.00
25_E 137_Q 3.075 1.00
42_C 99_F 2.971 1.00
122_Y 126_R 2.812 1.00
18_K 309_L 2.653 1.00
26_I 115_L 2.635 1.00
27_R 37_E 2.559 1.00
250_H 295_S 2.516 1.00
23_M 301_V 2.38 1.00
136_Q 142_P 2.371 1.00
148_S 214_H 2.361 1.00
22_I 305_V 2.36 1.00
81_M 92_V 2.317 1.00
145_E 208_K 2.311 1.00
85_L 294_C 2.307 1.00
124_M 234_E 2.258 1.00
42_C 96_C 2.242 1.00
123_D 126_R 2.18 1.00
28_R 31_A 2.146 1.00
151_Q 155_R 2.14 1.00
127_L 295_S 2.138 1.00
119_R 122_Y 2.128 1.00
29_F 134_L 2.087 1.00
171_E 175_K 2.079 1.00
148_S 151_Q 2.061 1.00
240_Y 245_L 2.04 1.00
259_Q 281_Q 2.037 1.00
57_E 70_N 2.028 1.00
26_I 134_L 1.981 1.00
147_L 152_A 1.973 1.00
29_F 33_R 1.968 1.00
132_D 146_S 1.948 1.00
129_N 133_D 1.947 1.00
15_N 311_A 1.945 1.00
29_F 137_Q 1.94 1.00
25_E 138_V 1.922 1.00
135_L 238_V 1.912 1.00
259_Q 263_E 1.909 1.00
211_F 238_V 1.889 1.00
128_M 146_S 1.877 1.00
20_A 31_A 1.83 1.00
227_T 234_E 1.826 1.00
125_Y 146_S 1.825 1.00
21_A 24_A 1.819 1.00
214_H 226_S 1.808 1.00
125_Y 129_N 1.795 1.00
129_N 146_S 1.793 1.00
32_D 137_Q 1.793 1.00
17_L 36_L 1.788 1.00
255_A 259_Q 1.773 1.00
260_Q 264_Q 1.758 1.00
117_W 297_V 1.74 1.00
147_L 212_V 1.73 1.00
13_I 17_L 1.726 1.00
226_S 234_E 1.716 1.00
14_P 18_K 1.706 1.00
264_Q 267_R 1.65 1.00
35_V 119_R 1.617 1.00
151_Q 154_L 1.61 1.00
39_E 97_R 1.607 1.00
171_E 174_A 1.591 1.00
166_K 170_R 1.571 1.00
152_A 212_V 1.559 1.00
150_Q 232_V 1.553 1.00
16_L 319_A 1.55 1.00
119_R 130_E 1.534 1.00
59_R 68_G 1.522 1.00
34_G 91_P 1.509 1.00
122_Y 295_S 1.508 1.00
260_Q 263_E 1.507 1.00
255_A 288_K 1.499 1.00
257_E 260_Q 1.491 1.00
259_Q 284_I 1.474 1.00
145_E 155_R 1.474 1.00
26_I 301_V 1.457 1.00
30_F 115_L 1.45 1.00
150_Q 154_L 1.444 1.00
217_A 225_I 1.436 1.00
59_R 70_N 1.429 1.00
165_D 168_Q 1.419 1.00
98_S 114_M 1.419 1.00
148_S 213_Y 1.406 0.99
156_Y 203_N 1.381 0.99
25_E 28_R 1.378 0.99
17_L 21_A 1.369 0.99
139_L 308_A 1.364 0.99
14_P 17_L 1.357 0.99
30_F 117_W 1.354 0.99
115_L 301_V 1.352 0.99
77_P 98_S 1.341 0.99
285_E 288_K 1.334 0.99
128_M 211_F 1.33 0.99
91_P 119_R 1.323 0.99
22_I 138_V 1.315 0.99
18_K 21_A 1.297 0.99
37_E 93_F 1.285 0.99
56_F 62_G 1.279 0.99
26_I 138_V 1.264 0.99
254_D 257_E 1.259 0.99
38_V 84_L 1.241 0.98
132_D 143_A 1.226 0.98
21_A 31_A 1.224 0.98
224_Q 253_T 1.219 0.98
22_I 308_A 1.216 0.98
272_R 275_P 1.212 0.98
16_L 89_C 1.209 0.98
172_V 176_L 1.201 0.98
134_L 138_V 1.2 0.98
217_A 261_R 1.196 0.98
225_I 257_E 1.193 0.98
135_L 304_L 1.188 0.98
77_P 114_M 1.185 0.98
43_M 71_L 1.182 0.98
38_V 89_C 1.175 0.98
144_A 238_V 1.162 0.97
261_R 264_Q 1.158 0.97
311_A 317_V 1.153 0.97
131_V 299_L 1.137 0.97
259_Q 288_K 1.136 0.97
132_D 144_A 1.127 0.97
18_K 22_I 1.127 0.97
311_A 316_E 1.126 0.97
133_D 136_Q 1.118 0.96
122_Y 127_L 1.118 0.96
250_H 253_T 1.106 0.96
20_A 24_A 1.1 0.96
35_V 91_P 1.097 0.96
273_G 276_Q 1.093 0.96
187_D 190_T 1.087 0.96
238_V 246_A 1.08 0.95
132_D 136_Q 1.08 0.95
291_M 294_C 1.075 0.95
172_V 175_K 1.067 0.95
82_K 92_V 1.065 0.95
81_M 94_Q 1.058 0.95
173_A 191_L 1.057 0.95
193_Q 197_T 1.049 0.94
57_E 72_W 1.042 0.94
133_D 137_Q 1.035 0.94
168_Q 171_E 1.035 0.94
77_P 80_H 1.033 0.94
40_T 94_Q 1.03 0.94
252_L 257_E 1.024 0.93
107_Y 243_I 1.022 0.93
214_H 234_E 1.022 0.93
261_R 265_D 1.017 0.93
86_V 291_M 1.016 0.93
223_A 249_F 1.015 0.93
252_L 261_R 1.013 0.93
28_R 137_Q 1.012 0.93
30_F 134_L 1.007 0.93
95_L 301_V 1.006 0.93
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
4ex5A20.97541000.075Contact Map0.713
4h02A80.97541000.087Contact Map0.876
3bjuA40.97231000.102Contact Map0.817
3a74A40.97231000.118Contact Map0.824
1e1oA10.97231000.121Contact Map0.711
3a5yA40.99381000.129Contact Map0.801
4j15A20.93541000.149Contact Map0.734
3i7fA20.94461000.15Contact Map0.692
1l0wA20.961000.151Contact Map0.797
1eovA10.94151000.154Contact Map0.664
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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