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OPENSEQ.org
TRPB - Tryptophan synthase beta chain
UniProt: P0A879 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG11025
Length: 397 (386)
Sequences: 4310
Seq/Len: 11.17
TRPB
Paralog alert: 0.73 [within 20: 0.03] - ratio of genomes with paralogs
Cluster includes: CYSK CYSM DPAL SDHD TDCB TRPB
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
251_G 323_D 4.448 1.00
123_S 130_C 4.353 1.00
212_T 373_V 4.273 1.00
331_E 360_K 3.694 1.00
72_T 371_L 3.467 1.00
61_K 72_T 3.382 1.00
260_H 266_E 3.357 1.00
172_E 175_R 3.224 1.00
129_K 155_E 3.063 1.00
133_Y 176_D 2.844 1.00
32_F 203_E 2.677 1.00
226_A 253_I 2.579 1.00
191_A 238_I 2.532 1.00
136_A 160_H 2.513 1.00
59_L 373_V 2.505 1.00
258_G 328_T 2.424 1.00
227_V 240_M 2.396 1.00
175_R 179_G 2.387 1.00
131_R 157_I 2.364 1.00
214_A 218_E 2.296 1.00
63_Q 67_A 2.295 1.00
56_P 214_A 2.228 1.00
12_F 318_S 2.224 1.00
47_D 51_N 2.215 1.00
206_R 210_E 2.215 1.00
136_A 164_A 2.215 1.00
131_R 155_E 2.207 1.00
51_N 219_R 2.19 1.00
225_D 370_Q 2.129 1.00
61_K 66_T 2.097 1.00
222_R 371_L 2.09 1.00
72_T 220_E 2.089 1.00
240_M 375_N 2.041 1.00
61_K 74_Y 2.02 1.00
271_L 317_N 1.991 1.00
105_E 176_D 1.972 1.00
150_R 155_E 1.964 1.00
366_P 370_Q 1.93 1.00
247_E 250_V 1.924 1.00
176_D 180_S 1.917 1.00
227_V 244_F 1.889 1.00
212_T 240_M 1.883 1.00
75_L 339_L 1.847 1.00
255_V 357_H 1.836 1.00
64_N 67_A 1.815 1.00
17_V 25_L 1.808 1.00
31_A 34_S 1.777 1.00
57_T 81_L 1.762 1.00
209_G 240_M 1.758 1.00
338_T 359_L 1.728 1.00
328_T 331_E 1.721 1.00
330_D 334_E 1.706 1.00
56_P 215_Q 1.705 1.00
216_I 373_V 1.699 1.00
253_I 323_D 1.666 1.00
245_I 322_A 1.654 1.00
73_L 358_A 1.651 1.00
35_A 100_R 1.613 1.00
8_Y 16_Y 1.611 1.00
185_H 197_Y 1.606 1.00
74_Y 371_L 1.605 1.00
209_G 243_D 1.59 1.00
83_G 121_L 1.563 1.00
220_E 371_L 1.563 1.00
22_M 26_R 1.559 1.00
64_N 342_H 1.543 1.00
180_S 184_A 1.54 1.00
226_A 251_G 1.534 1.00
241_F 320_G 1.532 1.00
365_N 370_Q 1.531 1.00
227_V 252_L 1.53 1.00
95_A 126_L 1.526 1.00
240_M 252_L 1.519 1.00
224_P 373_V 1.512 1.00
354_A 374_V 1.507 1.00
325_V 357_H 1.503 1.00
39_P 43_A 1.5 1.00
163_S 296_E 1.495 1.00
335_A 356_A 1.495 1.00
237_A 321_R 1.492 1.00
351_S 377_S 1.485 1.00
72_T 369_E 1.471 1.00
304_L 353_H 1.467 1.00
29_E 33_V 1.459 1.00
333_L 349_L 1.451 1.00
59_L 216_I 1.449 1.00
228_I 358_A 1.436 1.00
137_K 140_E 1.432 1.00
226_A 372_L 1.427 1.00
24_A 181_Y 1.416 1.00
357_H 361_M 1.413 1.00
327_I 356_A 1.411 1.00
213_K 247_E 1.396 0.99
160_H 164_A 1.394 0.99
336_F 352_S 1.391 0.99
95_A 106_I 1.377 0.99
180_S 183_T 1.346 0.99
15_M 26_R 1.342 0.99
65_I 355_L 1.318 0.99
315_Y 319_T 1.318 0.99
351_S 376_L 1.305 0.99
116_G 149_M 1.299 0.99
144_P 384_I 1.294 0.99
15_M 22_M 1.291 0.99
366_P 369_E 1.286 0.99
8_Y 11_E 1.267 0.99
40_E 43_A 1.266 0.99
339_L 355_L 1.266 0.99
33_V 37_K 1.257 0.99
48_L 93_G 1.254 0.99
78_E 377_S 1.251 0.99
104_T 183_T 1.248 0.99
228_I 253_I 1.24 0.98
140_E 143_S 1.24 0.98
30_E 34_S 1.239 0.98
35_A 40_E 1.229 0.98
225_D 371_L 1.227 0.98
124_A 130_C 1.221 0.98
53_A 81_L 1.219 0.98
337_K 391_L 1.208 0.98
260_H 264_T 1.207 0.98
285_P 318_S 1.206 0.98
119_S 123_S 1.202 0.98
33_V 199_T 1.201 0.98
216_I 220_E 1.201 0.98
313_H 324_Y 1.193 0.98
335_A 355_L 1.187 0.98
35_A 38_D 1.18 0.98
147_F 150_R 1.177 0.98
11_E 318_S 1.174 0.98
46_N 210_E 1.171 0.98
111_G 116_G 1.17 0.97
347_P 376_L 1.165 0.97
333_L 390_I 1.163 0.97
74_Y 220_E 1.157 0.97
171_N 175_R 1.157 0.97
216_I 224_P 1.156 0.97
47_D 50_K 1.145 0.97
254_G 324_Y 1.145 0.97
251_G 370_Q 1.142 0.97
11_E 276_V 1.138 0.97
216_I 371_L 1.136 0.97
52_Y 92_L 1.134 0.97
99_K 128_L 1.126 0.97
253_I 325_V 1.116 0.96
109_E 166_L 1.106 0.96
259_G 329_D 1.106 0.96
48_L 92_L 1.1 0.96
9_F 199_T 1.093 0.96
133_Y 172_E 1.093 0.96
338_T 342_H 1.09 0.96
94_Q 198_P 1.087 0.96
121_L 152_M 1.084 0.96
45_F 49_L 1.077 0.95
50_K 218_E 1.077 0.95
26_R 29_E 1.075 0.95
265_G 273_H 1.072 0.95
209_G 244_F 1.07 0.95
331_E 356_A 1.064 0.95
33_V 36_Q 1.064 0.95
356_A 360_K 1.057 0.95
339_L 347_P 1.055 0.95
42_Q 46_N 1.054 0.95
36_Q 39_P 1.049 0.94
358_A 374_V 1.041 0.94
74_Y 216_I 1.038 0.94
107_I 176_D 1.038 0.94
46_N 50_K 1.032 0.94
270_P 287_M 1.029 0.94
26_R 30_E 1.025 0.93
336_F 382_K 1.024 0.93
336_F 387_V 1.024 0.93
91_V 122_A 1.02 0.93
27_Q 30_E 1.019 0.93
357_H 360_K 1.018 0.93
27_Q 31_A 1.017 0.93
279_Y 284_A 1.015 0.93
168_D 171_N 1.015 0.93
237_A 317_N 1.015 0.93
301_S 332_A 1.013 0.93
226_A 370_Q 1.013 0.93
77_R 376_L 1.008 0.93
170_C 174_L 1.006 0.93
304_L 350_E 1.005 0.93
146_V 150_R 1.001 0.92
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
1qopB10.9951000.272Contact Map0.652
4negA20.98991000.287Contact Map0.758
1x1qA20.99241000.288Contact Map0.757
2o2eA20.99751000.291Contact Map0.738
1v8zA40.97481000.299Contact Map0.821
1tdjA10.82871000.365Contact Map0.684
1v71A10.80861000.372Contact Map0.701
3ss7X10.91441000.381Contact Map0.578
2gn0A40.83881000.383Contact Map0.813
2pqmA20.79851000.385Contact Map0.689
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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