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OPENSEQ.org
TOLC - Outer membrane protein TolC
UniProt: P02930 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG11009
Length: 493 (403)
Sequences: 7535
Seq/Len: 18.70
TOLC
Paralog alert: 0.81 [within 20: 0.14] - ratio of genomes with paralogs
Cluster includes: CUSC MDTP MDTQ TOLC
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
32_A 131_T 3.411 1.00
156_Y 187_L 3.06 1.00
149_Q 194_R 2.987 1.00
31_Q 210_N 2.801 1.00
163_T 180_R 2.795 1.00
374_Q 405_K 2.727 1.00
163_T 167_N 2.661 1.00
375_S 378_D 2.531 1.00
152_K 183_Y 2.447 1.00
145_L 190_E 2.403 1.00
149_Q 190_E 2.366 1.00
259_I 331_F 2.292 1.00
390_T 393_D 2.275 1.00
266_H 324_V 2.227 1.00
138_V 197_L 2.213 1.00
48_A 330_N 2.206 1.00
170_L 388_T 2.203 1.00
45_D 333_G 2.192 1.00
44_A 329_Y 2.166 1.00
157_R 160_D 2.165 1.00
38_E 340_S 2.147 1.00
270_L 310_F 2.102 1.00
143_D 146_S 2.098 1.00
41_K 337_Q 2.068 1.00
381_E 385_S 2.058 1.00
173_I 375_S 2.05 1.00
142_I 197_L 2.045 1.00
166_F 176_V 2.036 1.00
119_T 247_Q 2.022 1.00
51_E 322_S 2.008 1.00
52_K 326_Q 1.972 1.00
152_K 190_E 1.925 1.00
30_Q 34_L 1.911 1.00
374_Q 378_D 1.87 1.00
164_Q 382_A 1.855 1.00
144_V 406_Q 1.829 1.00
112_A 258_Q 1.82 1.00
108_L 258_Q 1.816 1.00
184_D 187_L 1.803 1.00
53_I 113_A 1.796 1.00
184_D 368_Q 1.793 1.00
145_L 194_R 1.769 1.00
141_A 200_A 1.75 1.00
156_Y 183_Y 1.744 1.00
118_V 246_L 1.726 1.00
111_K 250_L 1.726 1.00
156_Y 160_D 1.704 1.00
115_I 251_S 1.685 1.00
141_A 193_A 1.666 1.00
153_E 157_R 1.665 1.00
316_Q 320_V 1.66 1.00
118_V 121_Q 1.632 1.00
247_Q 251_S 1.614 1.00
145_L 197_L 1.612 1.00
359_S 411_A 1.608 1.00
140_N 413_Y 1.606 1.00
152_K 187_L 1.605 1.00
104_R 107_T 1.605 1.00
112_A 254_L 1.597 1.00
44_A 336_E 1.591 1.00
330_N 333_G 1.589 1.00
408_L 412_R 1.588 1.00
360_I 415_Y 1.583 1.00
24_N 27_Q 1.577 1.00
367_K 408_L 1.574 1.00
256_R 331_F 1.573 1.00
150_A 405_K 1.554 1.00
381_E 398_T 1.546 1.00
44_A 332_V 1.538 1.00
140_N 409_A 1.532 1.00
105_A 261_Q 1.53 1.00
396_D 399_T 1.52 1.00
40_R 336_E 1.512 1.00
347_Q 351_S 1.487 1.00
180_R 368_Q 1.482 1.00
177_Q 375_S 1.474 1.00
28_V 131_T 1.465 1.00
27_Q 30_Q 1.462 1.00
29_Y 132_A 1.46 1.00
363_I 408_L 1.445 1.00
142_I 201_V 1.443 1.00
168_V 386_V 1.442 1.00
114_G 117_D 1.43 1.00
131_T 204_L 1.43 1.00
159_L 183_Y 1.42 1.00
401_L 405_K 1.419 1.00
146_S 149_Q 1.414 1.00
33_R 128_I 1.408 1.00
26_M 30_Q 1.407 0.99
46_R 120_Y 1.401 0.99
166_F 173_I 1.396 0.99
108_L 261_Q 1.393 0.99
55_E 326_Q 1.392 0.99
147_Y 409_A 1.389 0.99
47_D 50_F 1.379 0.99
51_E 325_K 1.372 0.99
273_T 307_G 1.365 0.99
181_A 373_A 1.364 0.99
133_T 417_I 1.36 0.99
102_K 269_T 1.353 0.99
325_K 328_Q 1.342 0.99
159_L 180_R 1.337 0.99
115_I 254_L 1.331 0.99
153_E 156_Y 1.324 0.99
249_R 338_L 1.322 0.99
202_E 351_S 1.319 0.99
31_Q 208_T 1.318 0.99
48_A 333_G 1.317 0.99
331_F 335_S 1.317 0.99
197_L 201_V 1.317 0.99
23_E 28_V 1.316 0.99
39_L 127_L 1.314 0.99
146_S 150_A 1.313 0.99
159_L 163_T 1.304 0.99
174_T 380_M 1.303 0.99
60_L 106_L 1.296 0.99
155_I 186_V 1.296 0.99
145_L 149_Q 1.291 0.99
47_D 332_V 1.288 0.99
148_T 186_V 1.287 0.99
250_L 253_D 1.285 0.99
72_Y 85_N 1.284 0.99
29_Y 33_R 1.281 0.99
160_D 163_T 1.278 0.99
29_Y 128_I 1.273 0.99
332_V 335_S 1.268 0.99
340_S 344_S 1.265 0.99
367_K 371_V 1.264 0.99
377_L 401_L 1.261 0.99
259_I 324_V 1.258 0.99
368_Q 371_V 1.258 0.99
54_N 322_S 1.253 0.99
150_A 153_E 1.246 0.98
132_A 420_L 1.243 0.98
230_P 233_A 1.24 0.98
133_T 421_N 1.238 0.98
248_A 334_A 1.238 0.98
190_E 194_R 1.235 0.98
323_Q 326_Q 1.227 0.98
159_L 176_V 1.219 0.98
242_N 425_A 1.215 0.98
195_N 355_N 1.211 0.98
180_R 184_D 1.205 0.98
111_K 253_D 1.198 0.98
160_D 164_Q 1.197 0.98
206_Q 347_Q 1.196 0.98
53_I 110_E 1.188 0.98
374_Q 401_L 1.187 0.98
253_D 256_R 1.186 0.98
43_A 120_Y 1.186 0.98
126_T 130_N 1.185 0.98
138_V 201_V 1.183 0.98
121_Q 124_Q 1.183 0.98
367_K 412_R 1.182 0.98
41_K 336_E 1.18 0.98
51_E 329_Y 1.18 0.98
103_W 106_L 1.177 0.98
370_V 401_L 1.176 0.98
27_Q 31_Q 1.174 0.98
380_M 389_R 1.173 0.98
370_V 405_K 1.172 0.98
164_Q 167_N 1.169 0.97
31_Q 35_S 1.167 0.97
320_V 324_V 1.163 0.97
191_V 361_S 1.161 0.97
96_S 99_D 1.161 0.97
338_L 342_H 1.158 0.97
45_D 48_A 1.156 0.97
58_S 319_M 1.155 0.97
384_Y 391_I 1.152 0.97
415_Y 419_Q 1.152 0.97
177_Q 180_R 1.15 0.97
359_S 418_N 1.149 0.97
32_A 127_L 1.146 0.97
152_K 156_Y 1.144 0.97
46_R 117_D 1.138 0.97
46_R 113_A 1.133 0.97
370_V 408_L 1.131 0.97
199_N 355_N 1.13 0.97
64_L 93_L 1.13 0.97
364_N 368_Q 1.126 0.97
333_G 337_Q 1.124 0.97
183_Y 187_L 1.123 0.97
259_I 328_Q 1.121 0.97
326_Q 330_N 1.119 0.96
252_Q 338_L 1.113 0.96
349_V 422_I 1.107 0.96
107_T 257_E 1.105 0.96
382_A 385_S 1.099 0.96
315_Y 318_G 1.098 0.96
52_K 55_E 1.093 0.96
118_V 250_L 1.091 0.96
252_Q 335_S 1.088 0.96
245_L 345_V 1.088 0.96
410_N 414_N 1.083 0.96
356_I 415_Y 1.08 0.95
383_G 389_R 1.072 0.95
136_F 420_L 1.068 0.95
114_G 250_L 1.067 0.95
242_N 348_T 1.066 0.95
129_L 424_S 1.062 0.95
249_R 253_D 1.057 0.95
116_Q 119_T 1.051 0.94
144_V 410_N 1.05 0.94
53_I 57_R 1.049 0.94
252_Q 331_F 1.045 0.94
120_Y 124_Q 1.043 0.94
173_I 177_Q 1.038 0.94
25_L 132_A 1.038 0.94
327_A 330_N 1.034 0.94
44_A 47_D 1.031 0.94
377_L 381_E 1.031 0.94
362_S 414_N 1.029 0.94
242_N 345_V 1.028 0.94
366_Y 411_A 1.026 0.94
28_V 208_T 1.026 0.94
98_F 101_S 1.025 0.93
122_T 126_T 1.025 0.93
56_A 109_Q 1.024 0.93
339_E 342_H 1.021 0.93
55_E 323_Q 1.021 0.93
378_D 382_A 1.02 0.93
263_Q 324_V 1.02 0.93
143_D 412_R 1.018 0.93
313_P 316_Q 1.017 0.93
145_L 193_A 1.014 0.93
195_N 358_A 1.011 0.93
42_S 123_D 1.011 0.93
149_Q 153_E 1.01 0.93
266_H 320_V 1.006 0.93
137_N 417_I 1.003 0.93
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
1ek9A30.86821000.353Contact Map0.748
1yc9A10.86211000.408Contact Map0.621
3pikA10.8541000.415Contact Map0.679
4k7rA10.8541000.415Contact Map0.664
3d5kA30.89861000.42Contact Map0.814
3zbiC140.091314.40.97Contact Map0.124
2f1mA40.505113.20.971Contact Map0.772
1nhlA10.10956.40.974Contact Map0.2
3b5nC30.14250.976Contact Map0.303
1tzsX10.04673.90.977Contact Map
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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