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OPENSEQ.org
HYPE - Hydrogenase isoenzymes formation protein HypE
UniProt: P24193 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG10487
Length: 336 (321)
Sequences: 5154
Seq/Len: 16.06
HYPE
Paralog alert: 0.79 [within 20: 0.16] - ratio of genomes with paralogs
Cluster includes: HYPE SELD THIL
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
243_E 305_E 4.118 1.00
279_V 287_V 3.085 1.00
54_A 89_A 3.048 1.00
170_L 278_A 3.028 1.00
70_G 118_E 2.915 1.00
169_V 302_L 2.809 1.00
245_S 248_A 2.645 1.00
169_V 305_E 2.563 1.00
56_S 149_G 2.461 1.00
166_A 308_E 2.454 1.00
283_A 286_Q 2.453 1.00
241_G 307_V 2.425 1.00
171_L 288_L 2.42 1.00
166_A 240_C 2.395 1.00
171_L 300_A 2.337 1.00
242_I 306_V 2.319 1.00
288_L 300_A 2.302 1.00
169_V 284_A 2.277 1.00
69_G 143_L 2.201 1.00
168_D 217_K 2.196 1.00
280_E 283_A 2.185 1.00
42_D 155_A 2.18 1.00
235_A 242_I 2.138 1.00
25_E 121_R 2.137 1.00
118_E 121_R 2.119 1.00
284_A 302_L 2.093 1.00
243_E 307_V 2.09 1.00
217_K 280_E 2.088 1.00
164_L 218_A 2.078 1.00
55_F 95_S 2.047 1.00
234_F 278_A 2.042 1.00
281_R 305_E 2.032 1.00
231_V 242_I 2.007 1.00
215_G 283_A 1.999 1.00
285_E 289_A 1.998 1.00
170_L 242_I 1.992 1.00
243_E 315_A 1.99 1.00
74_K 119_T 1.963 1.00
171_L 302_L 1.956 1.00
231_V 244_L 1.952 1.00
118_E 122_A 1.951 1.00
240_C 306_V 1.926 1.00
178_D 186_L 1.887 1.00
39_A 128_V 1.873 1.00
169_V 281_R 1.864 1.00
111_A 114_T 1.851 1.00
165_T 280_E 1.837 1.00
28_A 90_I 1.824 1.00
120_A 127_I 1.764 1.00
170_L 276_V 1.737 1.00
40_R 54_A 1.722 1.00
98_F 109_L 1.72 1.00
153_I 158_H 1.706 1.00
95_S 129_T 1.697 1.00
154_P 157_I 1.674 1.00
50_G 155_A 1.667 1.00
70_G 111_A 1.661 1.00
286_Q 289_A 1.653 1.00
171_L 279_V 1.653 1.00
52_R 155_A 1.625 1.00
168_D 306_V 1.596 1.00
159_W 219_L 1.587 1.00
70_G 114_T 1.586 1.00
92_R 152_A 1.585 1.00
241_G 309_R 1.572 1.00
38_Q 87_S 1.566 1.00
56_S 84_V 1.56 1.00
71_N 115_S 1.531 1.00
217_K 278_A 1.53 1.00
189_Q 264_L 1.501 1.00
57_T 148_A 1.494 1.00
50_G 156_N 1.493 1.00
245_S 305_E 1.48 1.00
93_Y 128_V 1.469 1.00
219_L 275_L 1.465 1.00
55_F 150_M 1.465 1.00
165_T 217_K 1.453 1.00
213_I 290_A 1.448 1.00
114_T 118_E 1.444 1.00
288_L 302_L 1.44 1.00
175_T 178_D 1.432 1.00
285_E 288_L 1.419 1.00
84_V 89_A 1.411 1.00
163_T 217_K 1.398 0.99
164_L 306_V 1.379 0.99
38_Q 54_A 1.377 0.99
227_V 276_V 1.375 0.99
178_D 252_K 1.374 0.99
77_I 116_M 1.371 0.99
59_S 146_N 1.371 0.99
117_A 121_R 1.366 0.99
213_I 286_Q 1.364 0.99
284_A 288_L 1.363 0.99
86_V 219_L 1.358 0.99
171_L 284_A 1.344 0.99
210_L 287_V 1.339 0.99
55_F 148_A 1.337 0.99
231_V 235_A 1.334 0.99
52_R 153_I 1.33 0.99
90_I 208_Q 1.328 0.99
286_Q 290_A 1.324 0.99
168_D 280_E 1.317 0.99
164_L 234_F 1.312 0.99
227_V 231_V 1.305 0.99
78_C 119_T 1.294 0.99
76_A 145_I 1.293 0.99
289_A 293_S 1.277 0.99
119_T 122_A 1.27 0.99
54_A 84_V 1.262 0.99
219_L 277_I 1.26 0.99
245_S 315_A 1.251 0.99
289_A 292_H 1.248 0.99
164_L 278_A 1.245 0.98
81_A 149_G 1.238 0.98
40_R 158_H 1.237 0.98
80_T 149_G 1.235 0.98
302_L 305_E 1.231 0.98
81_A 125_I 1.231 0.98
214_P 287_V 1.231 0.98
157_I 212_D 1.208 0.98
54_A 153_I 1.207 0.98
218_A 234_F 1.199 0.98
30_P 41_L 1.187 0.98
112_V 116_M 1.178 0.98
96_C 116_M 1.171 0.98
160_G 163_T 1.168 0.97
169_V 279_V 1.164 0.97
110_K 114_T 1.163 0.97
94_L 147_T 1.159 0.97
251_V 256_R 1.158 0.97
250_P 256_R 1.156 0.97
135_V 144_F 1.152 0.97
42_D 50_G 1.147 0.97
84_V 149_G 1.141 0.97
52_R 158_H 1.14 0.97
120_A 125_I 1.14 0.97
288_L 292_H 1.139 0.97
74_K 78_C 1.138 0.97
209_T 294_H 1.127 0.97
278_A 306_V 1.123 0.97
209_T 212_D 1.119 0.96
107_E 111_A 1.116 0.96
51_D 154_P 1.116 0.96
74_K 122_A 1.113 0.96
246_E 266_A 1.111 0.96
259_C 266_A 1.095 0.96
173_S 291_L 1.094 0.96
100_L 109_L 1.091 0.96
94_L 149_G 1.087 0.96
43_L 152_A 1.067 0.95
18_L 110_K 1.06 0.95
180_G 272_E 1.059 0.95
171_L 287_V 1.057 0.95
93_Y 126_A 1.051 0.94
222_A 230_V 1.043 0.94
105_P 108_T 1.04 0.94
246_E 314_L 1.039 0.94
277_I 287_V 1.031 0.94
40_R 87_S 1.022 0.93
77_I 81_A 1.019 0.93
170_L 306_V 1.016 0.93
243_E 313_R 1.011 0.93
59_S 99_I 1.008 0.93
59_S 97_G 1.004 0.93
68_P 198_S 1 0.92
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
2z1uA10.98211000.204Contact Map0.699
2z1eA10.96431000.206Contact Map0.761
2rb9A40.98211000.262Contact Map0.783
3vtiC20.92561000.265Contact Map0.794
3fd5A20.96131000.325Contact Map0.68
2yydA20.89581000.335Contact Map0.753
3u0oA20.89881000.354Contact Map0.798
2z01A10.94051000.372Contact Map0.738
2btuA20.94051000.372Contact Map0.803
3m84A20.92561000.378Contact Map0.778
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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