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OPENSEQ.org
DDLB - D-alanine--D-alanine ligase B
UniProt: P07862 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG10214
Length: 306 (298)
Sequences: 11585
Seq/Len: 38.88
DDLB
Paralog alert: 0.89 [within 20: 0.46] - ratio of genomes with paralogs
Cluster includes: ACCC DDLA DDLB GSHB PUR2 PURK PURT RIMK
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
113_P 181_K 4.349 1.00
159_A 162_A 3.812 1.00
4_K 36_D 3.806 1.00
239_A 243_K 3.772 1.00
135_S 160_E 3.761 1.00
230_E 233_Q 3.114 1.00
74_G 92_A 2.876 1.00
104_W 244_A 2.83 1.00
242_L 246_T 2.79 1.00
231_A 235_A 2.765 1.00
74_G 78_L 2.746 1.00
106_G 247_T 2.598 1.00
139_L 160_E 2.58 1.00
115_V 136_A 2.465 1.00
235_A 239_A 2.462 1.00
236_N 239_A 2.418 1.00
236_N 265_Q 2.254 1.00
261_D 265_Q 2.228 1.00
124_K 164_Q 2.225 1.00
119_R 122_F 2.166 1.00
158_V 162_A 2.12 1.00
102_L 106_G 2.095 1.00
156_K 182_W 2.07 1.00
109_L 268_L 2.063 1.00
184_S 262_S 2.059 1.00
233_Q 236_N 2.022 1.00
5_I 84_T 2.006 1.00
132_A 136_A 1.988 1.00
104_W 268_L 1.978 1.00
190_V 237_L 1.964 1.00
158_V 184_S 1.953 1.00
243_K 246_T 1.948 1.00
99_R 102_L 1.919 1.00
75_M 79_M 1.89 1.00
164_Q 168_R 1.868 1.00
160_E 164_Q 1.865 1.00
6_A 40_V 1.813 1.00
186_P 202_R 1.806 1.00
87_G 90_A 1.792 1.00
134_I 139_L 1.751 1.00
235_A 238_Q 1.724 1.00
293_S 296_Q 1.722 1.00
133_E 136_A 1.72 1.00
76_L 81_L 1.702 1.00
171_F 175_E 1.69 1.00
240_L 266_F 1.687 1.00
105_Q 113_P 1.676 1.00
30_L 37_A 1.669 1.00
92_A 99_R 1.663 1.00
77_E 88_V 1.648 1.00
110_P 265_Q 1.635 1.00
192_I 245_W 1.581 1.00
101_K 180_E 1.579 1.00
87_G 251_K 1.571 1.00
4_K 38_Y 1.57 1.00
89_M 93_L 1.567 1.00
94_S 271_A 1.553 1.00
5_I 35_I 1.551 1.00
233_Q 260_L 1.543 1.00
110_P 240_L 1.541 1.00
5_I 30_L 1.539 1.00
2_T 60_I 1.538 1.00
96_D 146_S 1.523 1.00
98_L 102_L 1.52 1.00
142_I 156_K 1.518 1.00
93_L 99_R 1.512 1.00
188_F 260_L 1.507 1.00
118_T 122_F 1.49 1.00
93_L 103_L 1.489 1.00
102_L 105_Q 1.486 1.00
146_S 176_E 1.468 1.00
7_V 27_L 1.466 1.00
192_I 241_V 1.447 1.00
139_L 163_L 1.434 1.00
132_A 135_S 1.396 0.99
113_P 136_A 1.383 0.99
232_S 236_N 1.38 0.99
193_L 253_W 1.38 0.99
98_L 146_S 1.378 0.99
234_E 238_Q 1.376 0.99
260_L 266_F 1.375 0.99
157_V 166_A 1.369 0.99
229_L 233_Q 1.354 0.99
3_D 60_I 1.353 0.99
76_L 83_Y 1.346 0.99
2_T 82_P 1.318 0.99
139_L 159_A 1.309 0.99
105_Q 111_V 1.288 0.99
98_L 116_A 1.288 0.99
100_S 104_W 1.286 0.99
155_S 169_L 1.282 0.99
118_T 176_E 1.279 0.99
124_K 160_E 1.278 0.99
122_F 132_A 1.267 0.99
120_A 124_K 1.262 0.99
133_E 137_L 1.262 0.99
165_D 169_L 1.261 0.99
142_I 182_W 1.258 0.99
236_N 240_L 1.244 0.98
115_V 133_E 1.242 0.98
91_S 273_T 1.227 0.98
6_A 55_F 1.224 0.98
141_V 179_I 1.223 0.98
256_I 271_A 1.219 0.98
3_D 56_Q 1.216 0.98
190_V 238_Q 1.216 0.98
194_G 197_I 1.206 0.98
165_D 168_R 1.203 0.98
299_V 303_E 1.192 0.98
35_I 299_V 1.191 0.98
147_R 174_D 1.19 0.98
230_E 264_G 1.189 0.98
245_W 254_G 1.186 0.98
131_L 164_Q 1.169 0.97
103_L 247_T 1.169 0.97
27_L 37_A 1.167 0.97
74_G 89_M 1.167 0.97
169_L 172_Q 1.163 0.97
29_G 32_E 1.159 0.97
190_V 241_V 1.155 0.97
28_A 32_E 1.153 0.97
94_S 248_L 1.146 0.97
194_G 246_T 1.143 0.97
237_L 266_F 1.141 0.97
101_K 111_V 1.134 0.97
134_I 163_L 1.125 0.97
232_S 235_A 1.123 0.97
259_M 269_L 1.116 0.96
243_K 247_T 1.113 0.96
74_G 88_V 1.107 0.96
143_V 166_A 1.101 0.96
181_K 267_Y 1.09 0.96
120_A 164_Q 1.087 0.96
77_E 83_Y 1.086 0.96
190_V 200_S 1.079 0.95
183_L 267_Y 1.075 0.95
121_E 132_A 1.066 0.95
121_E 124_K 1.064 0.95
197_I 242_L 1.064 0.95
186_P 260_L 1.061 0.95
57_K 305_A 1.049 0.94
240_L 258_V 1.042 0.94
131_L 160_E 1.041 0.94
134_I 138_G 1.04 0.94
58_V 81_L 1.037 0.94
77_E 87_G 1.033 0.94
239_A 242_L 1.031 0.94
232_S 264_G 1.029 0.94
70_G 92_A 1.027 0.94
224_F 228_G 1.024 0.93
75_M 78_L 1.023 0.93
167_L 171_F 1.023 0.93
120_A 168_R 1.02 0.93
25_A 294_F 1.012 0.93
96_D 147_R 1.009 0.93
41_D 46_D 1.004 0.93
90_A 249_G 1.003 0.93
103_L 106_G 1.002 0.92
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
1iowA10.97711000.142Contact Map0.671
3tqtA20.99021000.152Contact Map0.735
4fu0A20.97711000.155Contact Map0.719
3i12A40.97711000.156Contact Map0.716
3e5nA10.97711000.161Contact Map0.631
2i87A211000.162Contact Map0.744
2fb9A10.95421000.162Contact Map0.684
1ehiA20.99021000.163Contact Map0.716
3k3pA20.97711000.165Contact Map0.726
3lwbA20.98041000.166Contact Map0.653
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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