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OPENSEQ.org
FRDA - Fumarate reductase flavoprotein subunit
UniProt: P00363 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG10330
Length: 602 (568)
Sequences: 2731
Seq/Len: 4.81
FRDA
Paralog alert: 0.67 [within 20: 0.02] - ratio of genomes with paralogs
Cluster includes: DHSA FRDA NADB
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
9_A 171_L 4.047 1.00
544_D 547_N 4.007 1.00
78_C 83_V 3.688 1.00
268_G 279_K 3.51 1.00
257_N 261_Y 3.345 1.00
365_D 369_E 3.318 1.00
86_F 386_L 3.313 1.00
20_A 190_V 3.296 1.00
464_C 520_A 3.251 1.00
84_D 88_H 3.007 1.00
261_Y 266_D 2.968 1.00
527_K 540_C 2.91 1.00
158_V 216_M 2.89 1.00
262_R 284_E 2.825 1.00
15_G 46_T 2.777 1.00
244_M 248_C 2.754 1.00
153_F 184_I 2.749 1.00
257_N 298_E 2.736 1.00
470_P 527_K 2.731 1.00
24_A 411_A 2.636 1.00
11_V 171_L 2.625 1.00
6_A 34_A 2.624 1.00
20_A 378_V 2.561 1.00
484_Q 517_E 2.533 1.00
463_G 475_K 2.518 1.00
256_V 318_R 2.453 1.00
477_I 520_A 2.408 1.00
191_M 362_I 2.325 1.00
34_A 153_F 2.278 1.00
470_P 474_Q 2.271 1.00
362_I 381_C 2.27 1.00
480_L 520_A 2.25 1.00
143_T 146_Q 2.245 1.00
24_A 408_G 2.232 1.00
218_L 518_C 2.221 1.00
376_F 410_Q 2.172 1.00
135_H 138_H 2.17 1.00
464_C 476_T 2.165 1.00
89_H 406_L 2.16 1.00
15_G 400_L 2.137 1.00
377_A 381_C 2.106 1.00
85_Y 406_L 2.076 1.00
474_Q 524_M 2.076 1.00
214_M 224_L 2.059 1.00
65_Y 122_I 2.055 1.00
32_K 151_Q 2.052 1.00
7_D 187_N 2.044 1.00
364_T 382_S 2.038 1.00
193_T 213_G 2.034 1.00
190_V 411_A 1.988 1.00
36_I 173_A 1.985 1.00
322_E 326_H 1.974 1.00
175_N 178_E 1.946 1.00
407_A 411_A 1.935 1.00
236_G 354_T 1.933 1.00
469_T 536_L 1.912 1.00
460_M 464_C 1.909 1.00
248_C 253_G 1.891 1.00
470_P 535_R 1.885 1.00
56_V 63_F 1.877 1.00
318_R 348_P 1.861 1.00
191_M 381_C 1.849 1.00
464_C 473_M 1.83 1.00
477_I 517_E 1.822 1.00
295_F 313_V 1.819 1.00
223_P 371_R 1.768 1.00
43_R 48_A 1.756 1.00
75_D 543_R 1.753 1.00
160_D 219_S 1.745 1.00
54_A 394_S 1.744 1.00
225_R 383_S 1.743 1.00
254_I 262_R 1.74 1.00
455_E 479_K 1.728 1.00
8_L 190_V 1.726 1.00
245_T 248_C 1.722 1.00
46_T 137_L 1.711 0.99
333_C 337_K 1.686 0.99
359_M 532_A 1.685 0.99
102_C 136_M 1.678 0.99
38_K 212_D 1.677 0.99
369_E 410_Q 1.669 0.99
219_S 511_H 1.664 0.99
217_A 222_V 1.664 0.99
175_N 182_V 1.664 0.99
86_F 403_F 1.659 0.99
74_G 78_C 1.657 0.99
93_E 96_Q 1.656 0.99
459_A 476_T 1.651 0.99
34_A 184_I 1.647 0.99
5_Q 185_R 1.645 0.99
144_S 152_R 1.641 0.99
456_M 480_L 1.64 0.99
173_A 184_I 1.639 0.99
82_V 86_F 1.63 0.99
199_V 519_M 1.627 0.99
120_M 394_S 1.625 0.99
102_C 135_H 1.613 0.99
470_P 540_C 1.605 0.99
224_L 227_M 1.602 0.99
196_A 358_T 1.592 0.99
370_T 377_A 1.588 0.99
212_D 511_H 1.58 0.99
455_E 486_R 1.567 0.99
6_A 9_A 1.551 0.99
326_H 333_C 1.55 0.99
297_H 301_K 1.537 0.99
82_V 386_L 1.535 0.99
322_E 345_V 1.535 0.99
404_G 408_G 1.523 0.99
245_T 332_I 1.52 0.99
191_M 375_L 1.517 0.99
469_T 472_L 1.513 0.98
481_A 517_E 1.499 0.98
8_L 24_A 1.495 0.98
479_K 482_E 1.48 0.98
82_V 366_Q 1.48 0.98
80_Q 84_D 1.477 0.98
66_H 70_T 1.471 0.98
477_I 521_H 1.471 0.98
522_S 552_T 1.471 0.98
38_K 508_E 1.47 0.98
255_L 351_V 1.469 0.98
38_K 210_T 1.469 0.98
166_G 373_K 1.462 0.98
67_F 71_V 1.461 0.98
484_Q 514_N 1.454 0.98
83_V 87_V 1.452 0.98
190_V 378_V 1.449 0.98
66_H 395_N 1.449 0.98
364_T 403_F 1.443 0.98
463_G 476_T 1.443 0.98
168_V 189_V 1.43 0.98
217_A 362_I 1.43 0.98
256_V 348_P 1.428 0.98
46_T 400_L 1.427 0.98
333_C 345_V 1.416 0.97
342_V 347_E 1.415 0.97
77_L 530_R 1.415 0.97
63_F 67_F 1.412 0.97
167_H 374_G 1.395 0.97
13_A 37_S 1.387 0.97
11_V 191_M 1.387 0.97
54_A 120_M 1.385 0.97
228_E 526_R 1.38 0.97
189_V 374_G 1.373 0.97
447_E 486_R 1.373 0.97
464_C 519_M 1.373 0.97
523_A 533_H 1.368 0.97
162_L 183_Q 1.361 0.96
22_I 144_S 1.359 0.96
201_R 458_L 1.358 0.96
8_L 411_A 1.355 0.96
227_M 519_M 1.341 0.96
156_H 175_N 1.339 0.96
191_M 217_A 1.336 0.96
7_D 32_K 1.334 0.96
517_E 521_H 1.33 0.96
186_A 189_V 1.329 0.96
477_I 524_M 1.328 0.96
378_V 407_A 1.327 0.96
57_A 126_W 1.326 0.96
476_T 520_A 1.323 0.96
317_L 320_L 1.323 0.96
200_Y 464_C 1.322 0.96
35_L 152_R 1.314 0.95
19_R 23_A 1.304 0.95
459_A 479_K 1.3 0.95
298_E 303_N 1.299 0.95
63_F 88_H 1.294 0.95
98_E 112_V 1.292 0.95
89_H 93_E 1.286 0.95
89_H 92_T 1.283 0.95
410_Q 413_E 1.283 0.95
530_R 540_C 1.272 0.94
262_R 265_Q 1.271 0.94
20_A 407_A 1.268 0.94
67_F 84_D 1.266 0.94
296_W 300_R 1.262 0.94
23_A 409_E 1.26 0.94
197_G 355_A 1.26 0.94
326_H 345_V 1.258 0.94
566_Y 569_V 1.257 0.94
36_I 171_L 1.255 0.94
530_R 541_T 1.254 0.94
160_D 216_M 1.252 0.94
474_Q 478_D 1.245 0.93
254_I 319_H 1.241 0.93
168_V 372_I 1.239 0.93
293_Q 534_Q 1.237 0.93
107_R 111_S 1.236 0.93
487_F 513_L 1.231 0.93
6_A 153_F 1.225 0.93
367_N 410_Q 1.218 0.92
8_L 31_A 1.215 0.92
145_L 154_D 1.214 0.92
125_T 394_S 1.214 0.92
267_Y 298_E 1.214 0.92
470_P 524_M 1.207 0.92
468_R 523_A 1.207 0.92
254_I 284_E 1.199 0.92
90_C 398_A 1.192 0.91
325_L 333_C 1.191 0.91
263_Y 295_F 1.189 0.91
204_T 236_G 1.186 0.91
471_E 475_K 1.185 0.91
71_V 78_C 1.183 0.91
161_I 189_V 1.18 0.91
16_A 378_V 1.179 0.91
120_M 125_T 1.178 0.91
85_Y 403_F 1.178 0.91
431_A 435_Q 1.173 0.90
453_R 457_G 1.172 0.90
156_H 173_A 1.172 0.90
459_A 483_L 1.17 0.90
93_E 405_R 1.168 0.90
18_L 35_L 1.162 0.90
458_L 462_E 1.157 0.90
23_A 408_G 1.155 0.90
475_K 479_K 1.149 0.89
364_T 377_A 1.142 0.89
408_G 411_A 1.142 0.89
326_H 337_K 1.141 0.89
325_L 345_V 1.139 0.89
86_F 398_A 1.138 0.89
63_F 87_V 1.131 0.88
463_G 479_K 1.126 0.88
226_D 551_H 1.121 0.88
474_Q 477_I 1.121 0.88
119_G 359_M 1.111 0.87
9_A 189_V 1.107 0.87
70_T 395_N 1.107 0.87
286_G 290_K 1.099 0.86
219_S 514_N 1.096 0.86
337_K 345_V 1.092 0.86
215_G 219_S 1.089 0.86
157_F 508_E 1.082 0.85
530_R 537_D 1.079 0.85
103_P 135_H 1.078 0.85
265_Q 281_K 1.078 0.85
443_Q 446_G 1.073 0.84
19_R 93_E 1.072 0.84
64_E 68_H 1.069 0.84
196_A 230_V 1.067 0.84
223_P 363_E 1.065 0.84
20_A 411_A 1.059 0.83
463_G 472_L 1.052 0.83
158_V 171_L 1.043 0.82
222_V 375_L 1.041 0.82
22_I 100_W 1.038 0.82
134_F 138_H 1.034 0.81
251_E 328_R 1.032 0.81
51_G 285_L 1.032 0.81
53_S 136_M 1.027 0.81
112_V 126_W 1.025 0.80
300_R 536_L 1.024 0.80
156_H 182_V 1.023 0.80
190_V 407_A 1.023 0.80
255_L 264_L 1.022 0.80
188_A 414_R 1.022 0.80
225_R 363_E 1.02 0.80
144_S 150_I 1.019 0.80
463_G 520_A 1.017 0.80
49_A 52_G 1.016 0.80
188_A 376_F 1.015 0.80
91_P 95_T 1.013 0.79
473_M 520_A 1.011 0.79
564_L 569_V 1.01 0.79
35_L 144_S 1.01 0.79
192_A 378_V 1.006 0.79
19_R 96_Q 1.006 0.79
431_A 434_E 1.003 0.78
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
1kf6A211000.243Contact Map0.771
2h88A20.93521000.258Contact Map0.759
2bs2A20.97181000.263Contact Map0.756
2wdqA30.93191000.273Contact Map0.78
1chuA10.86711000.351Contact Map0.785
3gyxA60.88541000.365Contact Map0.646
2e5vA20.78071000.407Contact Map0.805
1jnrA20.87711000.415Contact Map0.715
4c3xA80.62621000.628Contact Map0.672
1qo8A20.62791000.636Contact Map0.757
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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